Isolasi Dan Karakterisasi α-amilase Isolat Bakteri Amilolitik Asidofilik Dari Taman Nasional Rawa Aopa Watumaohai

Sapto Raharjo, Ardiansyah Ardiansyah, Agus Chahyadi

Abstract


Isolation and characterization α-amylase acidophilic amylolytic bacteria isolate from Rawa Aopa Watumohai National Park has been carried out. The aims of these studies are to know the characteristics of acidophilic amylolytic bacteria isolate, the optimum concentration of substrate and temperature α-amylase activity yielded, and specific activity in each enzyme purification steps. Activity of α-amylase was measured with DNS (Dinitrosalisilic acidI) method at λ 550 nm and the rate of protein enzyme was measured with Biuret method at λ 540 nm. The content of this research were obtained the isolate bacteria TR 1 which higher amylolytic activity is the colonies are pale-yellow with convexelevated, undulate, bacill sel, and negative Gram. The maximum activity and protein content obtained with (NH4)2SO4 extracted at 60% (w/v). The specific activity of α-amylase in crude extract was 0.038 U/mg enzyme, after extracted with (NH4)2SO4 60% (w/v) was 0.146 U/mg enzyme with purification fold 3.8, and after dialysis was 0.255 U/mg enzyme with purification fold 3.8, respectively. The optimum concentration of substrate (amylose) was 1.25% (w/v) and the optimum incubation temperature was 45oC.

Keywords : Rawa Aopa Watumohai National Park, acidophilic amylolytic bacteria, α-amilase


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References


Aiyer, P.V.D., 2004, Effect of C:N Ratio on Alpha Amylase Production by Bacillus licheniformis SPT 27, African J. of Biotechnology, Vol 3 (10) : 519-522.

Apun, K., Jong, B.C., dan Salleh, M.A. 2000, Screening and Isolation of A Cellulolytic and Amylolytic Bacillus from Sago Pith Waste, J. Gen. Appl. Microbiol, Vol. 46: 263-267.

Ballschmiter, M., Futterer, O., dan Liebl, W. 2006, Identification and Characterization of a Novel Intracellular Alkaline α-Amylase from The Hyperthermophilic Bacterium Thermotoga maritima MSB8, Appl. and Env. Microbiol, Vol 72 (3) : 2206-2211.

BKSDA Sultra. 2006, Taman Nasional Rawa Aopa Watumohai, BKSDA Sulawesi Tenggara, Kendari.

Chung, Y.C., Kobayashi, T., Kanai, H., Akiba, T., dan Kudo, T. 1995, Purification and Properties of Extracellular Amylase from The Hyperthermophilic Archaeon Thermococcus profundus DT5432, Appl. Environ. Microbiol, Vol. 61(4): 1502-1506.

Cordeiro, C.A.M., Martins, M.L.L., dan Luciano, A.B. 2002, Production and Properties of α-Amylase from Thermophilic Bacillus sp., Braz. J. Microbiol. Vol 33 (1).

Ekunsaumi, T. 1990, Laboratory Production and Assay of Amylase by Fungi and Bacteria, UW, Washington Country.

Fossi, B.T., Tavea, F., dan Ndjouenkeu, R. 2005, Production and Partial Characterization of a Thermostable Amylase from Ascomycetes Yeast Strain Isolated from Starchy Soils, African J. of Biotechnology, Vol. 4 (1): 14-18.

Hagihara, H., Igarashi, K., Hayashi, Y., Endo, K., Ikawakitayama, K., Ozaki, K., Kawai, S., dan Ito, S. 2001, Novel α-Amylase That Is Highly Resistant to Chelating Reagents and Chemical Oxidants from The Alkaliphilic Bacillus Isolate KSMK38, Applied and Environmental Microbiology, Vol 67 (4) : 1744-1750.

Reddy, N.S., Nimmagadda, A., dan Rao, K.R.S.S. 2003, An Overview of The Microbial α-Amylase Family, African J. of Biotechnology. Vol 2 (12) : 645-648.

Safey, E.M. dan Ammar, M.S. 2004, Purification and Characterization of α-amylase Isolated from Aspergillus falvus var.columnaris, Ass. Univ. Bull. Environ. Res. Vol. 7 No. 1.

Shaw, J.F., Lin, F.P., Chen, S.C., dan Chen, H.C. 1995, Purification and Properties of an extracellular α-amylase from Thermus sp., Bot.Bull. Acad. Sin, Vol. 36: 195–200


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