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Submitted
Sep 15, 2017
Accepted
Sep 15, 2017
Published
Dec 1, 2015
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Abstract

Chitosan was modified into nanoparticle chitosan in order to increase its absorptivity and solubility in water so that it can be applied in various fields. The objective of this research was to produce nanometer-sized chitosan, to characterizethe functional groups and the particle size of chitosan  as  well  as  to  test  its  antimicrobial  activity.  Chitosan  nanoparticles  were  synthesized through ionic gelation method by reacting chitosan with tripolyphosphate ions through ionic cross- linking reaction. The functional groups of chitosan were determined from its FTIR spectrum, the particle size was obtained from its X-ray diffractogram  and particle size analysis (PSA). Chitosan nanoparticles  with  the  smallest  size  based  on  XRD wereobtained  at  a  concentration  of  0.1% chitosan (CNPs 1) and chitosan 0.2% (CNPs 2) with the addition of 0.1 % tripolyphosphate ion. The sizes of chitosan nanoparticles,CNPs 1 and CNPs 2,obtained from the analysis of PSA were 224.68 and 204.32 nm, respectively. Polydisperse index value (pdi) of chitosan nanoparticles produced  were  0.226  and  0.261  (pdi  <0.5),  respectively.  The  results  indicate  that  chitosannanoparticles synthesized havehomogeneous particle size distributions. The test results against four types of pathogens showed that CNPs 2 had inhibitory activity against the three types of microbes, Staphylococcus aureus, Pseudomonas aeruginosa, and Malassezia furfur, but it was not active against the fungus of Candida albicans.

Keywords

Shells of crabs Chitosan nanoparticles Antimicrobials

Article Details

How to Cite
Fatahu, F., Natsir, H., & Taba, P. (2015). Synthesis and Characterization of Chitosan Nanoparticles from theshells of crabs(Portunus pelagicus) and Application as Antimicrobial. Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta), 8(2), 17-28. https://doi.org/10.20956/ica.v8i2.2469
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References

  1. Dai, T., Tanaka M., Huang,Y.Y., and Hamblin M.R. 2011. Chitosan preparations for wounds and burns: Antimicrobial and wound-healing effects. Expert Rev. Anti. Infect. Ther.
  2. , 857–879.
  3. Matheis F. J. D. P. Tanasale, Amos Killay, and Marsela S. Laratmase.
  4. Chitosan from shell of crabs (Portunus sanginolentus L.) as Adsorbent for Methylene Blue indicator. Indonesian J. Nature, 14 (2)
  5. : 165-171.
  6. Burkatovskaya M., Tegos GP., Swietlick E., Demidova TN., Castano AP. and Hamblin MR. 2006. Use of chitosan bandage to prevent fatal infections developing from highly contaminated wounds in mice. J. Biomaterials, 27, 4157-4164.
  7. Benhabiles M.S., Salah R., Lounici H., Drouiche N., Goosen M.F.A., and Mameri N. 2012. Antibacterial activity of chitin, chitosan and its oligomers prepared from shrimp shell waste. Food Hydrocolloid. 29, 48–56.
  8. Vinsova J. and Vavrikova E. 2011. Chitosan Derivatives with Antimicrobial, Antitumour and Antioxidant Activities - a Review. Curr. Pharm. Design. 17: 3596-3607.
  9. Andreas Y., Giraud L., Gerente C., and Le Cloirec P. 2007. Antibacterial Effects of Chitosan Powder: Mechanism of Action. J. Environ. Techno, 28 (12) ;1357-1363.
  10. Agrawal P, Strijkers GJ, Nicolay K. 2010. Chitosan-based systems for molecular imaging. Adv Drug Deliv Rev, 62, 42-58.
  11. Thassu, D., Y. Pathak, and M. Deleers (eds.). 2007. Nanoparticulate drug- delivery systems; An overview. In: Nanoparticulate Drug Delivery Systems, 1-32. New York. Informa Healthcare USA Inc.
  12. Mohanraj UJ and Chen Y. 2006. Nanoparticles - A Review, Tropical; 5 (1): 561-573.
  13. Wahyono D. 2010. Chitosan nanoparticles characteristicandthe effect of particle sizeat Ketoprofen access efficient[Thesis]. Institute of Agriculture Bogor; Magister Program. Bogor.
  14. Kumirska J., Weinhold M.X., Thöming J., and Stepnowski P. 2011. Biomedical Activity of Chitin/Chitosan Based Materials— Influence of Physicochemical Properties Apart from Molecular Weight and Degree of N-Acetylation. Polymers, 3, 1875–1901.
  15. Khan, T.A., Peh, K.K., and Ching H.S., 2002, Reporting Degree of DeacetylationValues of Chitosan: The Influence of Analytical Methods, Journal of Pharmacy and Pharmaceutical Science, 5 (3): 205-
  17. Sugita P., Wukirsari T., Sjahriza A.and Wahyono D.2009. Chitosan: Source of Biomaterial in Feature.IPB Press Inc. Bogor.
  18. Suptijah P., Agoes M. Jacoeb, and Rachmania D. 2011. Characterization Chitosan Nano from White Shrimp Shells (Litopenaeusvannamei) with Ionic Gelation Methods. Jurnal Pengolahan Hasil Perikanan Indonesia, 14 (2): 78-84.
  19. De Alvarenga, E.S. Characterization and properties of chitosan. In Biotechnology of Biopolymers; Elnashar, M., Ed.; In Tech: Rijeka, Croatia, 2011; pp. 91–108.
  20. Park, J.H., Saravanakumar, G., Kim, K.,and Kwon, I.C. 2010. Targeted delivery of low molecular drugs using chitosan and its derivatives. J. Adv. Drug Deliv. Rev.62: 28–41.
  21. Ko, JH., Yin, HY., An, JH., Chung, DJ. 2010. Characterization of Cross- Linked Gelatin Nanofibers through Electrospinning. Macromolecullar Research. 18(2): 137-143.