A Study on Entrapment Efficiency of Earthworms (Lumbricus rubellus) Extract in the Ethosomal Drug Delivery System

Sabrina Resky Pratiwi, Nur Ainiah, Hardyanti Hardyanti, Dini Rusdayanti Putri, Emilia Utomo

Abstract


Introduction

Earthworms (Lumbricus rubellus) extract is known to contain  bioactive protein as antibacterial compounds. One of the disadvantages of polar compounds is slow penetration into the skin layers which can be solved by formulating it in the form of ethosomal drug delivery system. The aims of this research was to get information about ethanol concentration that can give the highest entrapment efficiency of the ethosome. 

Methods

Earthworms powder was macerated using 50% ethanol for 3 days. The extract was  formulated into ethosome with variation of ethanol concentration that are 20%, 30%, 40%, and 50%, respectively. The measurement of entrapment efficiency was conducted by measuring the amount of active protein of earthworms extract that was entrapped in ethosome vesicles using spectrophotometer UV-Visible.

Results

The results showed that the highest entrapment efficiency was 72.58 % performed by formula which was made with 0.5 % extract, 2 % soy lecithin and 50 % ethanol.

Conclusions

The highest percentage of entrapment efficiency of earthworms extract ethosome was 72.58%.

Keywords : ethosome, ethanol concentration , entrapment efficiency, Lumbricus rubellus.

 

 


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References


Akib, N.I., Suryani, S., and Prawesti, N. 2015. Preparasi Fenilbutazon dalam Pembawa Vesikular Etosom dengan Berbagai Variasi Konsentrasi Fosfatidilkolin dan Etanol. Medula. 2 (1): 112-118.

Aldarraji, Q.M., Halimoon, N. and Majid, N.M. 2013. Antioxidant Activity and Total Phenolic Content of Earthworm Paste of Lumbricus rubellus (Red Worm) and Eudrilus eugenia (African Night Crawler). Journal of Entomology and Nematology. 5 (3): 33-37.

Asmawati and Pratiwi, S.R. 2016. Pengembangan Formula Ekstrak Cacing Tanah (Lumbricus rubellus) sebagai Sediaan Antiacne: EfekAntiinflamasi dan Antibakterinya. Laporan Penelitian PKMP. Universitas Hasanuddin.

Barupal, A.K., Gupta. V., and Ramteke, S. 2010. Preparation and Characterization of Ethosomes for Topical delivery of Aceclofenac. Indian Journal of Pharmaceutical Sciences. 72 (5): 582-586.

Cho, J.H., Park, C.B., Yoon, Y.G., and Kim, S.C. 1998. Lumbricin I. A Novel Proline-Rich Antimicrobial Peptide from The Earthworm: Purification, cDNA Cloning and Molecular Characterization. Biochimica et Biophysica Acta. 1408 (1): 67-76.

Immanita, S., Debora, K.., and Rochmanti, M. 2012. Effect of

Earthworms (Lumbricus sp.) Extract Antibacterial Activity Against the Bacteria Salmonella Typhii. Folia Medica Indonesiana. 48 (3):102-108.

Istiqomah, L., Herdian, H., Damayanti, E., Hayati, S.N., and Julendra, H. 2012. Inhibitory of Encapsulated Earthworm Extract (Lumbricus rubellus) on Pathogenic Bacteria in Vitro. Media Peternakan. 35 (1): 1-8.

Mathur, A., Verma, S.K., Singh, S.K., Prakash, A., Prasad, G.B.K.S., and Dua, V.K. 2011. Anti-inflammatory Activity of Earthworm Extracts. International Journal of Pharmaceutical Sciences and Research. 2 (2):278-281.

Parashar, T., Sachan, R., Singh, V., Singh, G., Tyagi, S., Patel, C., and Gupta, A. 2013. Ethosomes: A Recent Vesicle of Transdermal Drug Delivery System. International Journal of Research and Development in Pharmacy and Life Sciences. 2 (2): 285-292.

Patel, A., Sharma, R.K., Trivedi, M., Shivaprakash, and Panicker, A. 2013. Ethosomes: A Novel Tool for Transdermal Drug Delivery. Research Journal of Pharmacy and Technology. 6 (8): 838-841.

Rajput, A., Shriwas, S., Dwivedi, S. and Dubey, R. 2016. Formulation And Evaluation Of Ethosomes Of Plumeria Indica Linn. Flowers. Advances in Pharmacology and Toxicology. 17 (3): 45-48

Razavi, H. and Janfaza, S. 2015. Ethosome: A Nanocarrier for Transdermal Drug Delivery. Journal of Paramedical Sciences. 6 (2): 38-43.

Sujatha, V., Vishnuvaravidyadhar, T., Parvathi., M., and Reddy, S. 2014. A Review on Transdermal Drug Delivery System by Ethosomes. PharmaTutor Magazine. 2 (11): 50-55.

Sun, Z. 2015. Earthworm as a Biopharmaceutical: From Traditional to Precise. European Journal of BioMedical Research. 1 (2): 28-35.

Tiwari, R.K., Chauhan, N.S., and Yogesh, H.S. 2010. Ethosomes: A Potential Carriers for Transdermal Drug Delivery. International Journal of Drug Development and Research. 2 (2): 448-452.

Verma, P., Deshmukh, K.R., and Pandey, A. 2011. Entrapment Efficiency Study of Hydrophilic Molecule in Ethosomal Formulations. International Journal of Universal Pharmacy and Life Sciences. 1 (3): 51-56.

Vijayan, V., Kumar, K.J., Muralidharan, S., and Parasuraman, S. Formulation and Characterization of Novel Ethanolic Carrier for Improves Penetration of Serratiopeptidase. Research & Reviews: A Journal of Drug Formulation, Development and Production. 2 (1): 1-6.


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