Antibiotic Resistance of E. Coli Isolates from Broiler Chick's Cecum in Makassar City

Authors

  • Erdi Purwanto Maros Veterinary Centre, Jl. Dr. Ratulangi, Allepolea, Lau, Maros District, 90514, South Sulawesi, Indonesia
  • Dini Marmansari Maros Veterinary Centre, Jl. Dr. Ratulangi, Allepolea, Lau, Maros District, 90514, South Sulawesi, Indonesia
  • Dwi Kesuma Sari Study Program of Veterinary Medicine, Faculty of Medicine, Hasanuddin University, Jl. Perintis Kemerdekaan Km. 10, Makassar, 90245, South Sulawesi, Indonesia
  • Muhammad Hatta Department of Microbiology, Faculty of Medicine, Hasanuddin University, Jl. Perintis Kemerdekaan Km. 10, Makassar, 90245, South Sulawesi, Indonesia

Keywords:

antibiotic resistance, E.coli, chicken, broiler, Makassar

Abstract

Broiler chickens or broilers are the cheapest source of animal protein that can be obtained apart from eggs. The high demand for broiler chicken meat is fulfilled because modern broilers grow rapidly and are maintained massively and intensively. Massive maintenance systems make the spread of disease easier. Treatment of bacterial diseases using inappropriate antibiotics can lead to antibiotic resistance. This study aims to look at the antibiotic resistance of Escherichia coli (E. coli) bacteria from broiler chickens. E coli bacteria were isolated from 34 samples of broiler chicken cecum taken from several large traders in the city of Makassar. The E. coli isolates were then tested using Kirby-Bauer's diffuse disc method on 5 types of antibiotics from 5 classes of antibiotics namely: Ampicillin (β-lactam); Tetracycline (Tetracycline); Gentamicin (Aminoglycosides); Enrofloxacin (Flouroquinolone); Sulfamethoxazole-Trimethroprim (Sulfonamide). The inhibition zone formed was interpreted using the CLSI 2018 standard. The results of this study were 79.4% of the samples resistant to Ampisillin, 85.3% resistant to Tetracycline, 61.8% resistant to Sulfamethoxazole-Trimethroprim, 76.5% resistant to Enrofloxacin and 52.9% were resistant to Gentamicin. With 91.18% of the sample being resistant to at least 2 types of antibiotics.

References

Angulo, F., Nunneru, J., & Blair, H. (2004). Antimicrobial resistance in zzonotic enteric pathogens. Rev.SCi.Tech.Off.Int.Epiz, 23(2), 485–496.

Bahri, S., Masbulan, E., & Kusumaningsih, A. (2005). Proses Praproduksi sebagai faktor penting dalam menghasilkan produk ternak yang aman untuk manusia. Jurnal Litbang Pertanian, 24(1), 27–36.

Darsono. (1996). Deteksi Residu Oksitetrasiklin dan Gambaran Patologi Anatomi Hati dan Ginjal Ayam Kampung dan AYam Broiler yang Dijual di Lima Pasar Kotamadya Surabaya. Media Kedokteran Hewan, 12(3), 178–182.

Kusumaningsih, A., Murdiati, T., & Bahri, S. (1996). Jalur Pemasaran Obat Hewan pada Peternakan Ayam Burat di Beberapa Lokasi di Jawa Barat dan DKI Jaya. Hemera Zoa, 79(1–2), 72–80.

Marliana, N., Zubaidah, E., & Sutrisno, A. (2015). Pengaruh Pemberian Antibiotika saat Budidaya terhadap Keberadaan Residu pada Daging dan hati Ayam Pedaging dari Peternakan Rakyat. Jurnal Ilmu-Ilmu Peternakan, 25(2).

Marshal, B., & Levy, S. (2011). Food Animals and Antimicrobilas: Impacts on Human Health. Clinical Microbology Review, 25(4), 718–733.

Murdiati, T., Indaningsih, & Bahri, S. (1998). Contamination at Animal Products by Pesticides and Antibiotics. ACIAR Proceedings, 115–121.

Peterson, D., & Bonomo, R. (2005). Extended-Spectrum B-Lactamases: a Clinical Update. Clinical Microbology Review, 18(4), 657–686.

Siswanto. (2014). Kajian Resistensi Antimikroba dan Situasinya pada Manusia di Indonesia. Seminar Nasional Dan Diskusi Interaktif Resistensi Antimikroba. Jakarta.

Werdiningsih, S., Patriana, U., Ariyani, N., Ambarwati, & Nugraha, E. (2013). Pengkajian Residu Tetrasiklin dalam paha, Hati dan telur Ayam pada Beberapa Provinsi di Indonesia. Buletin Pengujian Mutu Obat Hewan No 19 Tahun 2013. Balai Besar Pengujian Mutu dan Sertifikasi Obat Hewan.

Downloads

Published

2019-10-13

Issue

Section

Articles