MOLECULAR CHARACTERIZATION OF GENE 16S rRNA MICRO SYMBIONTS

Authors

  • Ismail Marzuki Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University
  • Alfian Noor Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University http://orcid.org/0000-0002-5052-4393
  • Nursiah La Nafie Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University
  • M Natsir Djide Faculty of Pharmacy, Hasanuddin University

DOI:

https://doi.org/10.20956/mca.v16i1.956

Abstract

Molecular characterization studies have been conducted 16S rRNA gene micro symbiont of sponge origin Melawai Beach, Balikpapan in East Kalimantan. Objective analysis of histomorphological research, isolation-purification, molecular characterization of micro-symbiont genes in order to search symbiont bacteria that can live in extreme environments contaminated hydrocarbon waste. The research method that morphological identification, isolation-purification and molecular characterization of the 16S rRNA gene with Chain Reaction Polymerization method. The results of histo-morphological analysis concluded sponge samples with species of Callyspongia sp Isolation and purification mikro symbionts of sponge obtained 2 (two) isolates. Characteristics of Isolates 1; spherical shape, colonize and creamy, while isolates 2; jagged shape, oval and white colonies. Molecular characterization of the 16S rRNA gene by PCR, Bacillus subtilis strain BAB-684 identification for isolates one is the number of nucleotide pairs reached 899 bp and the degree of similarity in GenBank reached 89% homologous, while the second is a Bacillus flexus strain PHCDB20 isolates the number reached 950 bp nucleotide pairs with the degree of similarity in GenBank reached 99% homologous.Keywords: Callyspongia sp, micro-symbionts, characterization, gene Molecules

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Published

2015-04-01