Determination of tannin and saponin dosage for defaunation improvement feed fermentability

I.M. D. Wahyuni, A. Muktiani, M. Christianto


The research was conducted to evaluate the effect of addition of tannin, saponin or combination of tannin and saponin to the concentrate of the ration on the microbial population and fermentability of feed in vitro and to assess the best dosage of uses. The research was arranged according to completely randomized design with four treatments and 3 replications. The treatments were ration without tannin and saponin (T0), ration with 1.2% saponin (T1), ration with 0.5% tannin and 0.9% saponin (T2), ration with 1% tannin and 0.6% saponin, (T3), ration with 1.5% tannin and saponin 0.3% (T4) and ration with 2% tannin and 0% saponin. the results of the experiment showed that the addition of the tannin, saponin or their combination altered microbial population in the rumen. Protozoas population decreased significantly whereas bacterial population was increased. NH3 production increased while ratio of acetaic to propionic acid tended to decrease. Total gas production increased 62.59-69.35 ml/200 mg of dry matter. The addition of 1% tannin and 0.6 % saponin shows the best effect to control protozoa population and feed fermentability in the rumen.

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Arora, S. P. 1995. Pencernaan Mikroba pada Ruminansia. Diterjemahkan oleh R. Murwani. Gadjah Mada University Press, Yogyakarta.

Diaz A., M. Avendano and A. Escobar. 1993. Evaluation of Sapindus saponaria as a defaunating agent and its effects on different ruminal digestion parameters. Livest. Res. Rural Dev., 5: 1-6.

Herdian H., L. Istiqomah. A. Febrisiantosa., dan D. Setiabudi. 2011 Pengaruh penambahan daun Morinda citrifolia sebagai sumber saponin terhadap karakteristik fermentasi, defaunasi protozoa, produksi gas dan metana cairan rumen secara in vitro. JITV, 16: 99-104

Hiai, S., Oura, H., Nakajima, T., 1976. Color reaction of some sapogenins and saponins with vanillin and sulfuric acid. Planta Medica, 29: 116–122.

Hsu, J. T., G. C. Fahey Jr., N. R. Merchen and R. I. Mackie. 1991. Effects of defaunation and various nitrogen supplementation regimens on microbial number numbers and activity in t he rumen of sheep. J. Anim. Sci., 69: 1279-1289.

Hu, W. L., W. Yue-Ming, L. Jian-Xin, G. Yan-Qiu and Y. Jun-An. 2005. Tea saponins affect in vitro fermentation and metanaogenesis in faunated and defaunated rumen fluid. J. Zhejiang Univ. Sci., 6: 787-792.

Jayanegara, A., N. Togtokhbayar, H. P. S. Makkar and K. Becker. 2008. Tannins determined by various methods as predictors of methane production reduction potential of plants by an in vitro rumen vermentation system. Anim. Feed Sci. and Tech., 150: 230-237.

Jayanegara, A., A. Sofyan, H. P. S. Makkar dan K. Becker. 2009. Kinetika produksi gas, kecernaan bahan organik dan produksi gas metana in vitro pada hay dan jerami yang disuplementasi hijauan mengandung tanin. Media Peternakan, 32: 120-129.

Jouany, J. P. 1991. Defaunation of the rumen. In : The Rumen Microbial Metabolism and Ruminant Digestion. J. P. Jouany (editor). Institute Nationale de La Recherché Agronomique. INRA, Saint-Genes Champanelle.

Kurihara, Y., T. Takechi and F. Shibata. 1978. Relationship between bacteria and ciliate protozoa in the rumen of sheep fed on purified diet. J. Agric. Sci., 90: 373-381.

Makkar, H. P. S., 2003. Effect and fate of tannins in ruminant animals, adaptation to tannins, and strategies to overcome detrimental effects of feeding tannin-rich feeds. Small Ruminant Research, 49: 241–256.

Ogimoto, K. and S. Imai. 1981. Atlas of Rumen Microbiology. Japan Scientific Societies Press,Tokyo. l-231 pp.

Ørskov, E. R. and M. Ryle. 1990. Energy Nutrition in Ruminants. Elsevier Science Publishers Ltd. London.

Owens, F. N. and A. L. Goetsch. 1988. Ruminal fermentation. In The Ruminant Animal Digestive Physiology and Nutrition. D.C. Church Ed.Prentice Hall, Englewood Cliffs, New Jersey.

Pambayun, R., M. Gardjito, S. Sudarmadji dan K. R. Kuswanto. 2007. Kandungan fenol dan sifat antibakteri dari berbagai jenis ekstrak produk gambir (Uncaria gambir Roxb). Majalah Farmasi Indonesia. 18: 141-146.

Russell, J. B., and Rychlik, J.L. 2001. Factors that alter rumen microbial ecology. Science 292: 1119–1122.

Santoso, B. dan B.Tj. Hariadi. 2007. Pengaruh suplementasi Acacia mangium Willd pada Pennisetum purpureum terhadap karakteristik fermentasi dan produksi gas metana in vitro. Med. Peternakan, 30: 106-113.

Smith, A. H., J.A. Imlay, and R.I. Mackie. 2003. Increasing the oxidative stress response allows Escherichia coli to overcome inhibitory effect of condensed tannins. Appl. and Environ. Microb., 69: 3406-3411.

Suharti, S., D.A. Astuti dan E. Wina. 2009. Kecernaan nutrien dan performa produksi sapi potong Peranakan Ongole (PO) yang diberi tepung lerak (Sapindus rarak) dalam ransum. JITV, 14: 200-207.

Tilley, J. M. A, and R. A. Terry. 1963. A two stage technique for the in vitro digestion of forage.J. British Grassland Soc., 18: 104–111.

Wallace, R. J., N. R. McEwan, F. M. McIntosh, B. Teferedegne, and C. New Bold. 2002. Natural product as manipulators of rumen fermentation. Asian-Aus. J. Anim. Feed Sci. and Tech., 15: 1458-1468.

Wang, J. K., J-A. Ye. and Jian-Xin Liu. 2011. Effects of tea saponins on rumen microbiota, rumen fermentation, methane production and growth performance—a review. Trop. Anim. Health Prod., 44: 697–706.

Wei, L. H., L. Jian-Xin, Y. Jun-An, W. Yue-Min, and G. Yan-Qiu. 2005. Effect of tea saponin on rumen fermentation in vitro. Anim. Feed Sci. and Tech., 120: 333-339.

Widyobroto B. P., S. P. S. Budhi dan A. Agus. 2007. Pengaruh aras undegraded protein dan energi terhadap kinetik fermentasi rumen dan sintesis protein mikroba pada sapi. J. Indon. Trop. Anim. Agric., 32: 194-200.

Widyobroto, B. P. 1995. Degradasi protein dalam rumen dan kecernaan protein dalam intestinum. Dalam : Kursus Singkat Teknik Evaluasi Pakan Ruminansia, Fak. Peternakan UGM, Yogyakarta.

Wina, E., S.Muetzel and K. Becker. 2005. The Impact of Saponin-Containing Plant Materials on Ruminant Production-A review: 1-13.

Wiseman, J. and W. J. A. Cole. 1990. Feedstuff Evaluation. Butterworth, London.

Woodward, S. L., G. C. Waghorn, M. J. Ulyatt, and K. R. Lassey. 2001. Early indications that feeding lotus will reduce methane emissions from ruminants. In: Proceedings of the The New Zealand Society of Animal Production. ACIAR, Adelaide, pp. 23–26.


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