The Production of Chitosan from Shrimp Shell Waste and Its Formulation in Patch DosageForm Combined with Aloe vera Extract as Antiinfection Agent
Abstract
Chitosan can be obtained from chitin isolated from shrimp waste through the process of deacetylation of chitin. Chitosan can be used as a patch base material because of its biocompatibility and biodegradability, and has antibacterial activity. The combination of Chitosan and Aloe vera extracts may be useful in patch dosage forms as wound dressings that have antiinfective activity. The research methods was laboratory experimental which consists of : chitosan was obtained from shell waste sequentially by deproteinisation, demineralisation, and deacetylation processes, and analyzed its characteristic, respectivel; Aloe vera gel was extraction by maceration methods using ethanol as solvent; patches were formulated using 2 % chitosan in 1.5 % glacial acetic acid, 1.6 % Aloe vera extracts and 10 % glycerin and evaluated its physical propoperties, skin iritation test, and antibacterial test against S.aureus. The results showed that percentage yield of chitosan was 32.67%, with characteristics; Its viscosity 93.33 cps, pH 4, 30 % deacetylation degree. Percentage yield of Aloe vera extract was 22.73%, containing acemannan, and had antibacterial activity against S.aureus. The result of patch evaluation showed that the thickness of patch was 0.45 mm, 114 times folding endurance, moisture uptake of 45.03%, moisture content of 23.64%, safe to use, and diameter of inhibition zone was 6.39 mm against S.aureus. So, combination of Aloe vera and Chitosan from shrimp shell as patch may use as antiinfection wound healing.
Keywords : Aloe vera L.; chitosan; wound dressing; patch; shrimp shell waste
References
Abbaas.A.A.F. 2015. Dynamic Assessment of Biocidal Properties of Silicon-Chitosan-Containing Hydrogels. International Journal of Innovative Research in Science, Engineering and Technology. 4(4): 2045-2050
Ahing.F.A., and N.Wid. 2016. Extraction and Characterization of Chitosan from Shrimp Shell Waste in Sabah. Transactions on Science and Technology. 3(1-2): 227-237.
Alami, R. and Permatasari, L. 2016. Industry Pharmaceuticals : Chitosan as an Alternative Replacement Gelatin Capsules on Shell. Journal of Medical and Bioengineering.5(1):67-71.
Arunkumar, S. and. Muthuselvam, M. 2009. Analysis of Phytochemical Constituents and Antimicrobial Activities of Aloe vera L. Against Clinical Pathogens. World Journal of Agricultural Sciences.5(5):572-576.
Bellamkonda, V., Vidya, S.R.G., and Vijaya, C. 2017. Chitosan from Shrimp Bio-Waste: Potential Antibacterial Agent. International Journal of Informative & Futuristic Research. 4(5):6398-6403.
Choche, T., Shende A., and Kadu, P. 2014. Extraction and Identification of Bioactive Components from Aloe barbadensis Miller. Journal of Pharmacognsoy and Phytochemistry. 2(1):14-23.
Choudhury. R., et al. 2012. Staphylococcal Infection, Antibiotic Resistance and Therapeutics. Intech. 247-272.
Daniel.E.A.K., and M.R.Hamblin. 2016. Chitin and Chitosan: Production and Application of Versatile Biomedical Nanomaterials. NCBI Journal. 4(3): 411-427
Danujatmiko.Z. et al. 2014. The Prospect of Shrimp Shell Waste as Raw Material in Gelatin Production. ARPN Journal of Engineering and Applied Sciences. 9(11): 2035-2038.
Devika.R., and J.Koilpillai. 2014. Etiology of Wound Healing and Hypertrophic Scar Formation. International Journal of Pharmaceutical Science and Health Care. 3(4): 78-86.
Fathima, S.A., Begum, S., and Syeda, S.F. 2017. Transdermal Drug Delivery System. International Journal of Pharmaceutical and Clinical Research.9(1):35-43.
Hong, S., et al. 2017. Chitosan-Based Multifunctional Platforms for Local Delivery of Therapeutics. Marine Drugs Journal.15(60):1-6.
Kataria.K. et al. 2014. In vivo Wound Healing Performance of Drug Loaded Electrospun Composite Nanofibers Transdermal Patch. International Journal of Pharmaceutics ELSVIER. 1-9.
Mahor.G., and S.A.Ali. 2016. Recent Update on The Medicinal Properties and Use of Aloe vera in The Treatment of Various Ailments.Bioscience Biotechnology Research Communications. 9(2): 273-288.
Rahman, S., et al. 2017. Aloe vera for Tissue Enggineering Applications. Journal of Infectional Bimaterials. 8(6):1-17.
Rajan.P.A. et al.2016. Protective Efficacy of A Novel Alpha Hemolysin Subunit Vaccine (AT62) Against Staphylococcus aureus Skin and Soft Tissue Infections. Vaccine, ELSEVIER: 1-6.
Sachan.R., and M.Bajpai. 2013. Transdermal Drug Delivery System: A Review. International Journal Research Development Pharmacy. 3(1): 748-765
Sharma.K. et al.2015. Aloe vera ad Penetration Enhancer. International Journal Drug Delivery and Research. 7(1): 31-34.
Silva. et al. 2013. An Investigation of The Potential Application of Chitosan/Aloe-based Membranes for Regenerative Medicine. Acta Biomaterialia ELSEVIER. 9:6790-6797.
Takeo.M. et al. 2015.Wound Healing and Skin Regeneration. Cold Spring Habor Perspective in Medicine.5: 1-12.
Tudose, A., et al. 2009. Regenerative Properties of Aloe vera Juice on Human Keratinocyte Cell Culture. Farmacia. 57:590–596.
Varshney.S., and S.Dhyani. 2015. Medicinal Herbs Having Incredible Wound Healing Effects. International Journal of Research. 6(5): 573-579.
Vijayalakshmi.P. 2015. Incidence of Staphylococcus aureus in Surgical Site Infections in A Teaching Hospital. 4(4): 32-34.
Yuan.Y. et al. 2011. Deacetylation of Chitosan:Material Characterization and In Vitro Evaluation via Albumin Adsorption and Pre-Osteoblastic Cell Cultures. MDPI Journal. 4:1399-1461
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