Issue

Vol. 2 No. 1 (2018): International Journal of Applied Biology

Date Log

Submitted
Jun 26, 2018
Published
Jul 28, 2018

Production of Bioethanol From Kappaphycus alvarezii Algae by Using Pichia kudriavzevii Yeast

https://doi.org/10.20956/ijab.v2i1.4391
Nur Fadillah
Biology Department, Mathematic and Science Faculty, Hasanuddin University
WInda Lestari Taufan
Biology Department, Mathematic and Science Faculty, Hasanuddin University
Muh Syahdan Aska
Biology Department, Mathematic and Science Faculty, Hasanuddin University

Corresponding Author(s) : Nur Fadillah

nurfadillahdils@gmail.com

International Journal of Applied Biology, Vol. 2 No. 1 (2018): International Journal of Applied Biology

Abstract

Kappaphycus alvarezii is a red algae that can be used as alternative raw material for bioethanol production. This is because K. alvarezii contains a high carbohydrate that reaches 60%. This study aims to determine the effect of fermentation nutrition and fermentation duration of hydrolysis results of K. alvarezii algae on pH changes, sugar levels, cell biomass and ethanol content. There are two methods was used in this research, namely hydrolysis method and fermentation method. The hydrolysis method was used α-amylases enzyme which is 150 KNU/L with 0.5%, 1.0%, 1.5% and 2.0% concentrations. The next method, K. alvarezii algae was fermented by Pichia kudriavzevii and used Gandasil-D® as an anorganic suplementation and yeast extract which is 0 g/L, 1 g/L, 2 g/L and 3 g/L. The results showed that         K. alvarezii can be fermented into bioethanol after enzymatic hydrolysis process. The optimal ethanol content was produced at 48 hours of incubation duration.
References
  1. Arad, S.M., Levy-Ontman, O. 2010. Red Microalgal Cell-Wall Polysaccharides: Biotechnological Aspects. Curr.Opin.Biotechnol. 21 (3): 358–364.
  2. Chang, V., Patrick, N. O., Swee-Sen, T. 2017. The Properties Of Red Seaweed (Kappaphycus Alvarezii) And Its Effect On Mammary Carcinogenesis. Biomedicine & Pharmacotherapy. 296-301.
  3. Del Monaco, S.M., Maria E. Rodriguez, Chistian A. Lopes. 2016. Pichia kudriavzevii as a Representative yeast of North Patagonian Winemaking Terrior. Journal International of Food Microbiology. 230: 31-39.
  4. Fayaz, M., K.K. Namitha, K.N. Chidambara Murthy, M. Mahadeva Swamy, R. Sarada, S. Khanam, P.V. Subbarao, G.A. Ravishankar. 2005. Chemical composition iron bioavailability, and antioxidant activity of K. alvarezii (Doty). Journal Agriculture Food Chem. 53: 792–797.
  5. Gonzalez, C.F., Farina, J.I. & Figueroa, L.I.C. 2008.Optimized Amylolytic Enzymes Production in S. fibuligera DSM-70554.Enzyme and Microbial Technology. 42: 272–277.
  6. Hayashi, L., A.A. Santos, G.S.M. Faria, B.G. Nunes, M.S. Souza, A.L.D. Fonseca, P.L. M. Barreto, E.C. Oliveira, Z.L. Bouzon. 2011. K. alvarezii(Rhodophyta, Areschougiaceae) cultivated in subtropical waters in Southern Brazil. J. App. Phys. 23: 337–343.
  7. Jang, J.S., Cho, Y.K., Jeong, G.T., Kim, S.K., 2012a. Optimization of saccharification andethanol production by simultaneous saccharification and fermentation (SSF)from seaweed, Saccharina japonica. Bioprocess Biosyst. Eng. 35, 11–18.Jannah, A., M. 2010.Proses Fermentasi Hidrolisat Jerami Padi Untuk Menghasilkan Bioetanol.Jurnal Teknik Kimia. 17(1): 44.
  8. Kim, S.W., Hong, C., Jeon, S. & Shin, H. 2015. High-yield Production of Biosugars from Gracilaria verrucosa by Acid and Enzymatic Hydrolysis Processes. Bioresource Technology. 196: 634–641.
  9. Kumar, S., Gupta, R., Kumar, G., Sahoo, D., Kuhad, R.C., 2013.Bioethanol productionfrom Gracilaria verrucosa, a red alga, in a biorefinery approach.Bioresour.Technol. 135: 150–156.
  10. Lee, Sang-Bum and Gwi, T. J. 2016.Production of Biosugar from Red Macro-Algae Eucheuma cottonii Using Acid-Hydrolysis.Microbiolgy and Biotechnology Letters. 44(1): 48-54.
  11. Loreo, Y. C., Ferrari, M. D., Guigo, M., Fajardo, L., Larnaudie, V., Ramirez, M. B. & Gerreiro, J. M. 2013.Evaluation of Sweet Potato for Fuel Bioethanol Production: Hydrolysis and Fermentation. Springer Plus. (2): 1-11.
  12. Meinita, M. D. N., Ji-Young, K., Gwi-Taek, J., Hyun, M. K., Sung, M. P., Yong-Ki, H. 2012. Bioethanol Production from the Acid Hydrolysate of the Carrageenophyte Kappaphycus alvarezii (cottonii). Journal of Applied Phycology. 24(4): 857-862.
  13. Munoz, J., Y. Freile-Pelegrin, D. Robledo. 2004. Mariculture of K. alvarezii(Rhodophyta, Solieriaceae) Color Strains in Tropical Waters of Yucatan, Mexico. Agriculture. 239, 161-177.
  14. Nicolau, A., Georgescu, L. & Bolocan, A. 2010. Impact Of Bioprocessing On Rice. Food Technology. 35(1) 19-26.
  15. Pandey, A., Nigam, P., et al. 2000. Advances in microbial amylases.Biotechnol. Appl. Biochem. 31: 135-152.
  16. Pickering, T.D., P. Skelton, Sulu, J.R., 2007. Intentional Introductions of Commercially Harvested Alien Seaweeds, Botani Marien. 50. 338–350.
  17. Ra, C.H., Jeong, G.T., Shin, M.K., Kim, S.K., 2013. Biotransformation of 5- hydroxymethylfurfural (HMF) by Scheffersomyces stipitis during ethanol fermentation of hydrolysate of the seaweed Gelidium amansii.Bioresour. Technol. 140: 421–425.
  18. Ramachandran, V., Pujari, N., Matey, T., & Kulkarni, S. 2013.Enzymatic Hydrolysis for Glucose-A Review. International Journal of Science, Engineering and Technology Research. 10(2): 1937-1942.
  19. Ravanal, C., Salazar, O., Mäki-Arvela, P., Mikkola, J. P., Lienqueo M. E. 2016.Comparison of different types of pretreatment and enzymatic saccharification of Macrocystis pyrifera for the production of biofuel. Algal Res. 13: 141–7.
  20. Sankh, S., Meikadhan Tinru, Saurabh, S., &Vidyah, R., 2013. Biodiesel Production from a Newly Isolated Pichia kudriavzevii strai.. Industrial Biotechnology Group Reliance Life Science Put. Ltd. Nan Mumbai. India.
  21. Vanegas, C.H., Hernon, A., Bartlett, J., 2015. Enzymatic and Organic Acid Pretreatment of Seaweed: Effect on Reducing Sugars Production and nn Biogas Inhibition. Int. J. Ambient Energy. 36, 2–7.
  22. Widodo, P., Chairul.S. and Reni, Y. Produksi Bioetanol Dari Nira Nipah Skala 50 Liter Dengan Penambahan Tween 80 Dan Ergosterol Pada Proses Fermentasi Menggunakan Saccharomyces Cerevisiae. 2015. JOM Teknik. 2:2. 1-8.
  23. Wu, F.C., Wu, J.Y., Liao, Y.J., Wang, M.Y., Shih, I.L., 2014. Sequential acid andenzymatic hydrolysis in situ and bioethanol production from Gracilariabiomass.Bioresour. Technol. 156, 123–131.
Read More
Author biographies is not available.
Download this PDF file
PDF
Statistic
Read Counter : 799 Download : 692

Downloads

Download data is not yet available.