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Vol. 3 No. 1 (2019): International Journal of Applied Biology

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Submitted
Feb 13, 2019
Published
Jun 29, 2019

Inhibition of Juice Komba-Komba (Chromolaenaodorata) Leaf Against Growth of Bacteria Staphylococcus aureus

https://doi.org/10.20956/ijab.v3i1.6062
Reni Yunus
Poltekkes Kemenkes Kendari
Muhammad Hilam
Satya Darmayani

Corresponding Author(s) : Reni Yunus

reniyunus@poltekkes-kdi.ac.id

International Journal of Applied Biology, Vol. 3 No. 1 (2019): International Journal of Applied Biology

Abstract

Nowadays many plants can be used as traditional medicines to overcome various diseases including infections, because the use of traditional medicines is relatively safer compared to drugs derived from chemicals. One of the local plants in Southeast Sulawesi that has been empirically used as a medicine for wounds is a race of plants. Komba-komba leaves (Chromolaena odorata) containcompounds alkaloid, flavonoids, tannins, phenols, triterpenoids, saponins and steroids which have their respective functions in inhibiting the growth of microorganisms or antibacterial. This study aims to determine the inhibition of komba-komba leaf extract (Chromolaena odorata) on the growth ofbacteria Staphylococcus aureus. This type of research is experimental laboratories. The method used was agar diffusion with 5 treatment concentrations, namely komba-sheep leaf extract concentration (Chromolaena odorata) 20%, 40%, 60%, 80% and 100%, positive control (tetracycline) and negative control (aquadest) and repetition . The results showed that there was a inhibition zone of komba-komba leaf extract (Chromolaena odorata) on the growth ofbacteria Staphylococcus aureus at a concentration of 20% at 2 mm, a concentration of 40% at 3.7 mm, a concentration of 60% at 4.7 mm, a concentration of 80% 6.2 mm and a concentration of 100% by 8 mm.
References
  1. Afrianti, R, R. Yenti, and L. Afriani, 2010., Preliminary studies of leaves of ethanol are important for wound healing, STIFL Research Report, Padang.
  2. Ayesha. 2015. Inhibitory Power of Pandan Wangi Extract (Pandanus amaryllifolius Roxb.) Against Staphylococcus aureus Bacterial Growth. Faculty
  4. Makassar UNHAS Dentistry. Thing 23
  5. Ajizah, Aulia; Thihana; Mirhanuddin. 2007. Potential of ironwood extract (Eusideroxylonzwageri) in inhibiting In Vitro Staphylococcus aureus bacterial growth.On line. Available at:.http://bioscientiae.unlam.ac.id/v4n1/v4n1_ajizah.pdfEncryption (Accessed, 5 January 2018).
  6. Apriani. 2012. Review of the Plant Library Komba - Komba (Chromolaena odorata). Accessed pdf on August 2, 2017.
  7. Apriyadi Tri Erza. 2010. The Risk of Staphylococcus aureus in Traditional Food Ready to Eat and Evaluate Its Presence in Uduk Rice. Faculty of Agricultural Technology Bogor Agricultural University p. 6.
  8. Aulia, Ismi Arsyi. 2008. Antibacterial activity test Ethyl acetate fraction Ethanolic extract of Arbenan leaves (duchesnea indica (andr.) Focke) against Staphylococcus aureus and Pseudomonas aeruginosa Multiresisten Antibiotics along with Thin Layer Chromatography Profiles. On line. Available at:http://etd.eprints.ums.ac.id/1517/1/K100040115.pdf. Thesis (Accessed, 5 January 2018)
  9. Awoyinka. 2007. Traditional Plants as Antibacterials for Treatment. Penebar Swadaya: Jakarta.
  10. Bachtiar Subhchan Yusuf, Tjahjaningsih, Nanik Sianita. 2012. Effect of Brown Algae Extract (Sargasus sp) on Escherichia coli bacterial growth. Faculty of Fisheries and Marine Airlangga University. Journal of Marine and Coastal Science, 1 (1) pp. 53-60.
  11. Brooks GF, Butel JS, Morse SA. Medical microbiology book 1. Interpreting: Mudihardi E, et al, editor. Jakarta: Salemba medika; 2005, p. 317-22
  12. Brooks GF, Carroll C, Butel JS, Morse SA, Mietzner A. Melnick & adelberg's medical microbiology 25years . USA: Mc Graw Hill; 2010, pp. 617
  13. Cappuccino, JG & Natalie, S. 2013. Biology Laboratory Manual; language transfer, Nur Miftahurrahmah. Jakarta: EGC.
  14. Dalimartha, S., 2000. Atlas of Plants Indonesian Medicine Edition 2. Jakarta: Trubus Agiwidya.
  15. Djajadisastra, Joshita, et al., 2009.Tropic Gel Formulation from Nerii Folium Extract in Anti-Acne Preparations. Indonesian Pharmacy Journal Vol.4 No.4 July 2009: 210-216. Universitas Indonesia Faculty of Mathematics and Natural Sciences.
  16. Dwidjoseputro D. 1994. Basics of Microbiology. Djambat, Jakarta.
  17. Hadi, M., JW Hidayat, K. Baskoro. 2000. Test the Potential of Eupatorium odoratum Leaf Extract as an Alternative Insecticide Material: Toxicity and Effects on the Larvae of Heliothis armigera. Hubner. Journal of Science and Mathematics. Undip Faculty of MIPA. Semarang
  18. Harbone, JB, 1987., Phytochemical Method Determination of Modern Analysis of Plants, Translated by Kosasih, Padmawinata, Issue of ITB, Bandung.
  19. Jawetz, et al., 2010. Medical Microbiology. translated Mudihardi, E., Kuntaman, Wasito, EB, Mertaniasih, NM, Harsono., S., Alim sardjono, L., Edition XXV, 198, Publisher Salemba Medika, Jakarta.
  20. Jumiarni Wa Ode, Oom Komalasari. 2017. "Exploitation of Types and Utilization of Medicinal Plants in Muna Society in Muna City Settlement" Traditional Medicine Journal, 22 (1) 2017 p. 45
  21. Kurniawan, B., Aryana, WF, 2015. Binahong (Cassia alata L) as inhibitor of Escherichia coli Growth. Majority, 4(4), 100-104.
  22. Kusuma, IW, Kuspradini, H., Arung, ET, Aryani, F., Min, YH, Kim, JS, Kim, YU, 2011. Biological Activity and Phytochemical Analysis of the Three Indonesian Medicinal Plants, Murraya koenigii, Syzygium polyanthum andZingiber purpurea. Journal of Acupuncture and Meridian Studies, 4(1), 7579.
  23. Loggia, RD, Tubaro A., Dri P., Zilli C., and Del Negro P. 1986. The role of flavonoids in the antiinflammatory activity of Chamoliarecutita. Plant flavonoids in biology and medicine: biochemical, pharmaceutical and structure-activity relationships. Alan R. Liss, Inc. p. 481-4.
  24. Madigan, MT, JM, et al. 2009.Brock Biology of Microorganisms, Pearsons enjamin Cummings, San Francisco, pp. 779
  25. Nugerahita Nindya. 2009. Prevalence of Skin Disease and its Treatment in Several RWs in Petamburan Village, Central Jakarta. University of Indonesia Faculty of Mathematics and Natural Sciences Depok 2009. Department of Pharmacy,
  26. Pratiwi ST. Pharmaceutical microbiology. Jakarta: Erlangga; 2008, p. 42-3, 188-91
  27. Purwati and Undri R. 2009. Screening of Secondary Metabolite Compounds and Antioxidant Activity Test of Ethylacetate Wedusan Leaf Extract (Euphatorium odoratum) Molecules, chemistry study program, MIPA department, Faculty of Science and Engineering UNSOED vol. 4.No. 2. November, 2009: 94-104.
  28. Purwiyanto Anna ISMSi. 2013. Practical Chemistry Oceanography Module. Faculty of Mathematics and Natural Sciences Sriwijaya University.
  29. Rahman Aminul. 2017. Effects of Ointment of Kirinyuh Leaf Extract (Euphatorium odaratum) on the Healing of Vegetable Wounds in Laying Hens (Gallus leghorn). Veterinary Medicine Study Program, Faculty of Medicine, Hasanuddin University, Makassar 2017.
  30. Robinson, T, 1995., High Plant Organic Content, Edition VI, ITB, Bandung.
  31. Sagala, NS 2009. Utilization of White Bunag Shrubs (Chromolena odarata) Against Growth and IOFC In Ramsun Quail (Cortunix-cortunix japonica) Age 1 to 42 Days. USU, Medan.
  33. Samaranayake. 2012. Essential Microbiology For Dentistry 4th ed. China: Elsevier; 2012, pp. 125
  34. Simanjuntak, MR 2008. Extraction and Fractionation of the Components of Senduduk Plant Leaf Extract (melastoma malabathricum. L) and Testing the Effect of Cream Preparations on Burning Healing. Thesis. University of North Sumatra. Field. 85 p.
  35. Sugiyono. 2011. Quantitative and Qualitative Research Methods (Print No. 14). Bandung: Alfabeta
  36. Tedy Nurwalidin Aka. 2005. Effectiveness of Antibacterial Ethanol Extract and Ethyl Acetate Ketapang Leaf (Terminalia catappa l.) Against Salmonella Typhy and Staphylococcus epidermidis. Thesis of the Science and Technology Faculty and Sunan Kalijaga Technology.
  37. Thamrin, M., S. Asiklin and M. Willis. 2013."Plant Kirinyuh (Chromolaena odorata) As a Vegetable Insecticide for Controlling Grayworms (Spodoptera litura)". Journal of vegetable insecticides, 22 (7) 2013 p. 113
  38. Toy Torar SS, Benedictus S. Lampus, Bernat SP Hutagalung. 2015. The Inhibition Test of Gracilaria sp Seaweed Extract Against Staphylococcus aureus Bacterial Growth. E-GiGi Journal (eG). Volume 3 Number 1 January - June 2015. Page 156.
  39. Vital, PG, and W. L, Rivera, 2009.Antimicrobacterial activity and citoxicity of chromolaena odorator (LF) King and Robinson and Uncaria perrottetii (A. Rich) merr. Extracts, Available online at http; // www.academicjournal.org/JMPR Journal of Medicial Plant Research vol. 3 (7), pp. 5511-518.
  40. Yuniarti Tuty. 2012. Media and Reagensia of Teaching Materials Department of Health Analyst Polytechnic Ministry of Keshatan Kendari.
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Author Biography

Reni Yunus, Poltekkes Kemenkes Kendari
Department of Health Analyst, Poltekkes kemenkes Kendari
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