Issue

Vol. 3 No. 1 (2019): International Journal of Applied Biology

Date Log

Submitted
Feb 18, 2019
Published
Jun 29, 2019

Lectin Protein Spodoptera litura Activity After Exposured by Biopesticide from Mirabilis jalapa

https://doi.org/10.20956/ijab.v3i1.6080
Dina Maulina
Universitas Lampung
Sutiman B Sumitro
Muhammad Amin
Sri Rahayu Lestari

Corresponding Author(s) : Dina Maulina

dina.maulina@fkip.unila.ac.id

International Journal of Applied Biology, Vol. 3 No. 1 (2019): International Journal of Applied Biology

Abstract

Spodoptera litura is folifagus agricultural pest with a tendency of resistance towards chemistry compound. This becomes a reason in the efforts to overcome it. The use of botanical material becomes one of the alternatives in controlling its population. The plant of Mirabilis jalapa has the active biopesticide compound that becomes its alternative prevention. This plant has the potential chemistry compound as biopesticide which works toward weakening immunity that is ended by the death of S. litura.  This research aims to analyze the existence of lectin protein as an indicator of immunity reaction’s activation Spodoptera litura after exposing biopesticide M. jalapa. Lectin test was conducted by using spot-test hemagglutination assay (HA) then was seen its speed forming of titer. The result of research shows that lectin was on the part of hemolimf S. litura supernatant. The result test shows that on the concentration 0.2% binding of lectin, carbohydrate and erythrocyte cells of vertebrate formed faster. The speed of titer forming was influenced by the number of hemosit. This is caused by immulectin receptors were on the cell’s surface. Therefore, exposing M. jalapa can induce lectin activation which functioned as the recognizing receptor of strange object which directly bound with carbohydrate related to the reaction of body immunity.Key words: biopesticide Mirabilis jalapa, lectin, immunity system, Spodoptera litura.
References
  1. Anggraeni, T., Melanie. & Putra, R. E. 2011. Cellular a Humoral Immune Defenses of Oxya japonica (Orthoptera: Acrididae) to Entomophathogenic fungi Metharizium anisopliae. Enthomological Research, 41, 1 - 6.
  2. Ashida M & Brey P. 1997. Recent advances in research on the insect prophenoloxidase cascade. Molecular Mechanisms of Immune Responses in Insects (ed. by P Brey & D Hultmark), pp. 135–171. Chapman & Hall, London, UK.
  3. Bai, Y., Yan, R., Ke, X., Ye,G., Huang, F., Luo, Y., & Cheng, J. 2011. Effect of Transgenic Bt Rice on Growth, Reproduction, and Superoxide Dismutase Activity of Folsomia candida (Collembola: Isotomidae) in Laboratory Studies, Journal Economic of Entomology, 104, 1892 - 1899.
  4. Boman, H. G. 1986. Antibacterial immune proteins in insects. Immune Mechanisms in Invertebrate Vectors (ed. by AMLackie), pp. 45–58. Symposia of the Zoological Society, London, UK.
  5. Cerenius, L & Soderhall, K 2004. The prophenoloxidase-activating system in invertebrates. Immunological Reviews 198: 116–126.
  6. Chapman, R. F. 2009. The Insect Structure and Function 4thEdition. Cambrigde University.
  7. Dutcher, J. D. 2007. A review of Resurgence and Replacement Causing Pest Outbreaks in IPM. General Concept in Intergrated and Disease Management, 27-43. Entomology Departement, University of Georgia, Tifton GA, USA.
  8. Gillot, C. 2005. Enthomology Third Edition. University of Saskatchewan- Springer.
  9. Gonzales, I., Santoyo, & Aguilar, A.C. 2011. Prophenoloxidase: A Key Component of The Insect Immune System, Journal of Entomologia Experimentalis et Applicata, 142, 1 - 16.
  10. Horne, P. A. & Page, J. 2008. Integrated Pest Management for Crops and Pastures, Victoria: Landlink Press.
  11. Kandagal, A. S. & Khetagoudar, M. C. 2011. Study on Lavarsidal Activity of Weed Extract Against Spodoptera litura. Journal of Environmental Biology. Vol. 34, 253-257.
  12. Kather, H. F. 2012. Prospect of Biopesticides in Insect Pest Management. Journal Pharmacologia. Vol. 3 (12): 641-656. Doi: 10.5567/phrmacologia.2012.641-656.
  13. Kumar, S. & Singh, A. 2015. Biopesticide: Present status and Future Prospect. Journal Fertilizer and Pesticide. Vol. 6 (2). Doi: 10. 4172/jbfbp. 1000e129.
  14. Kumar, S. 2012. Biopesticide: A Need for Food and Evironmental Safety. Journal Biofertilizer and Biopesticide. Vol. 3 (4). Doi: 10.41772/2155-6202. 1000e107.
  15. Leng, P., Zhang, Z., Pan, G. & Zhao, M. 2011. Applications and Development Trens in Biopesticide. African Journal of Biotechnology. Vol. 10(86), pp. 19864-19873.
  16. Marmaras, V.J. & Lampropoulou, M. 2009. Regulator and Signaling in Insect Haemocyte Immunity, Departement of Biology, University of patras, Journal of Cell Signaling, 17, 8-16.
  17. Maulina, D & Anggraeni, T. 2014. The Effect of The Combination of Two Biological Control Agents, Mirabilis jalapa and Bacillus thuringiensis, to Spodoptera exigua’s Immune Respons and Their Mortality. AIP Confference Proceeding-International Conferences Mathematics and Sains: Bandung.
  18. Maulina, D. 2013. Response of Cellullar Immune Spodoptera litura toward Biopesticide Mirabilis jalapa and Its Mortality rate When Combined with Bacillus thuringiensis. Master Thesis SITH-ITB. Bandung: ITB.
  19. Mei, Z. X., & Qiu, H. B. 2002. Insecticide resistance of the Common Cutworm (Spodoptera litura) and Its Control Strategies. Enthomological Knowledge. http://www.cnki.com.cn
  20. Nappi A.J. & Christensen B.M. 2005. Melanogenesis and associated cytotoxic reactions: applications to insect innate immunity. Insect Biochemistry and Molecular Biology 35: 443–459.
  21. Nathan, S. S., Chung, P. G., & Murugan, K. 2004. Effect of Botanical Incecticides and Bacterial Toxin on the Gut Enzyme of the Rice Leaffolder Cnaphalocrocis medinalis. Journal of Phyparasitica. Vol. 35 (5): 433-443.
  22. Prasetyo, D. B. 2006. Exposure Influence of Ethanol Extract of Leaves Mirabilis jalapa toward Woof Consumption, Weight and Growth Speed of Larva and also Pupa Stage and Percentage of Imago emergence Spodoptera exigua hubner. Mini Thesis of Biology Department Student. ITB.
  23. Romeis, J., Bartsch, D., Bigler, F., Candolfi, M. P., & Gielkens, M. M. C. 2008. Assesment of Risk of Insect-Resistant Transgenic Crpos to Nontarget Arthropods. Enthomology Publications. IOWA State University.
  24. Schreiner, I. 2000. Cluster Caterpillar (Spodoptera litura [Fabricius]), Agricultural Pest of The Pacific ADAP.
  25. Shapiro, M. 2000. Enchancement in activity of Homologus and Heterologus Baculoviruses Infectious to Beet Armiworm (Lepidotera: Noctuidae) By an Optical Brighttener, Journal of Economic Enthomology, 93, 572 - 576.
  26. Sparck, T.C & Nauen, R. 2014. IRAC: Mode of Action Classification and insecticide resistance. Pesticide Biochemistry and Physiology, 121 (2015) 122-128
  27. Suharsono. 2011. Sensitivity of Tolerant Soybean Strains Saturated with Water toward Spodoptera litura F. Research Center of Legumes and Tubers.
  28. Suryani, I & Anggraeni, T. 2014. The Effect of Leaf Biopesticide Mirabilis jalapa and Fungi Metarhizium anisopliae to Immune Response and Mortality of Spodoptera exigua Instar IV. AIP Proceeding-International Conferences Mathematics and Sains : Bandung.
  29. Sussman, A. 1949. The functions of tyrosinase in insects. Quarterly, Review of Biology 24: 328–341.
  30. Tanada, Y & Kaya, H. K. 1993. Insect Pathology. Academic Press, INC-Harcourt Brace Jovanovich, Publishers.
  31. Vivanco, M. J. 1999. Antviral and Antiviroid Activity of MAP-Containing Extracts from Mirabilis jalapa Roots, Plant Diseases, Department of Plant Pathology and Biotechnology Institute, University Park.
  32. Wyatt G. 1961. The biochemistry of insect hemolymph. Annual Review of Entomology 6: 75–102.
  33. Yu QX. 2004. Immulectin-2, a pattern recognition receptor that stimulate hemocyte encapsulation and melanization in the tobacco hornworm, Manduca sexta, Journal of Dev Comp Immunol, Jull 1,28 (9): 891-900. DOI: 10.1016/j.dci.2004.02.005.
  34. Yu QX. 2005. A Novel C-type immulectin-3 from Manduca sexta is translocated from hemolimph into the cytoplasma hemocyte, Journal of Insect Biochem Mol Biol, Apr, 35 (4): 285-95. DOI: 10.1016/j.ibmb.2005.01.004.
  35. Yu, QX. & Kanost, M. R. 2000. Immulectin-2, a Lipopolysaccharide-spesific Lectin from an Insecat, Manduca sexta, Is Induced in Response to Gram negative bacteria. Journal of Biological Chemistry. Vol : 275, 37373-37381. Doi: 10.1074/jbc.M003021200.
Read More
Author biographies is not available.
Download this PDF file
PDF
Statistic
Read Counter : 531 Download : 480

Downloads

Download data is not yet available.