• Ajuk Sapar Department of Chemistry, Tanjungpura University – Pontianak 78124
  • Alfian Noor Department of Chemistry, Hasanuddin University – Makassar, 90245
  • Nunuk Hariani Soekamto Department of Chemistry, Hasanuddin University – Makassar, 90245
  • Ahyar Ahmad Department of Chemistry, Hasanuddin University – Makassar, 90245
  • Tri Aryono Hadi Division of Marine Resources, Research Center for Oceanography (RCO), LIPI, Jakarta 14430



Toxicity assessment have been conducted on nine sponges species collected from Spermonde Archipelago namely Kapoposang Island, Samalona Island and Lanjukang Island. Toxicity assessment was using Brine Shrimp lethality Test (BST) method againts sponge extracts. Theresults test were expressed as 50% lethal concentration (LC50) to Artemia salina and correlated with prospecting extract potential as anticancer. All extracts obtained from smallscale extraction by methanol. Test results as follows: Acanthostrongylophora ingens (24.830 μg/mL), Hytios erectus (46.140 μg/mL), Stylissa massa (>1000 μg/mL), Theonella sp (35.010 μg/mL), Niphates olemda (87.500 ppm), Haliclona (Reniera) fascigera (78.160 μg/mL), Petrosia (Petrosia) sp (3.008), Callyspongia (Euplacella) biru (0.515 μg/mL). The test results showed that seven species are potential as anticancer with LC50 value ≤ 500 ppm and only Stylissa massa extracts was not potential as anticancer.


Garson, M. J., 1994. The Biosynthesis of Spons Secondary Metabolites: Why It is Important ? In: Soest, R. W. M. van, Th. M. G. van Kempen and J. C. Braekman, Sponss in Time and Space. Proc. 4th Int. Porifera Congr. Rotterdam: Balkema.

Ghisalberti EL. (1993). Detection and isolation of bioactive natural products. In S. M. Colegate, & R. J. Molyneux (Eds.), Bioactive Natural Products: detection, isolation and structure elucidation. Boca Raton: CRC Press, pp 15-18

Jinzhong X, Masateru Hasegawa, Ken-ichi Harada, Hisayoshi Kobayashi, Hiroshi Nagai, and Michio Namikoshi, 2006. Melophlins P, Q, R, and S: Four New Tetramic Acid Derivatives, from Two Palauan Marine Sponges of the Genus Melophlus. Chem. Pharm. Bull 54(6) 852-854

Martina S. T., Himawan T., Latif A. A., and Retnowati R., 2015. Toxicity and phytochemistry test of methanol extract of several plants from Papua using Brine Shrimp Lethality Test (BSLT). Journal of Chemical and Pharmaceutical Research. 7(4):866-872

Meyer, B.N. 1982. Brine Shrimp Agregaat Convenient General Bioassay for Active Plant Constituens. Planta Med 45. 31-34.

Mohamed A. Ashour, Ehab S. Elkhayat, Rainer Ebel, Ru Angelie Edrada, and Peter Prokschc, 2007. Indole alkaloid from the Red Sea sponge Hyrtios erectus. Arkivoc (xv) 225-231, ISSN 1551-7012

Soest, R. W. M. van and J. C. Braekman, 1999. Chemosystematics of Porifera: A Review. Memoirs of The Queensland Museum 44: 569-589. Brisbane