THE POTENTIAL BIODEGRADATION HYDROCARBONS OF PETROLEUM SLUDGE WASTE BY CELL BIOMASS SPONGE Callysppongia sp.
Abstract
The toxicity of petroleum sludge is a serious threat to marine life. Necessary concrete stepspetroleum contamination reduction through a search of potential marine materials degrade
toxic components hydrocarbons. The sponge is one potential material reducing toxic
properties of petroleum contamination. The research objective was to determine the
potential Callyspongia sp sponge against the degradation of petroleum hydrocarbon
sludge. Four variations of treatment used to determine the potential of sponge biomass,
namely: the treatment I, II, III and IV. Indicators degradation is the formation of gas, the
smell fermentation, pH changes and increase in the absorbance of the suspension of media
degradation. The level degradation each treatment is determined gravimetric method and
the known hydrocarbon component changes by using GC-MS. Unidentified gas, the smell
of fermentation and changes in media of pH on average degradation occurs on the 15 days
of contact for all treatments. Data showed degradation occurs absorbance maximum at 20-
25 days to contact. The highest degradation rates indicated by IV treatment (26.93 %), III
(24.84 %), II (22.59 %), and I: (18.20 %). Found 20 kinds of components in the waste
sludge aliphatic petroleum to form a homologous series nC10-nC30, and two aromatic
components, namely the 2.7-dimethyl naphthalene and 1.4-dimethyl azulene. Callyspongia
sp sponge biomass concluded at the highest IV treatment degrading aliphatic
hydrocarbons, then treatment III, II and I, while the aromatic components are not degraded
by biomass Callyspongia sp sponge at all treatments.
References
Ramdhani, Y., 2012. Kajian awal pemanfatan limbah lumpur minyak bumi (Sludge oil) menjadi bahan bakar padat, Laporan hasil penelitian JBPTPPOLBAN, Jakarta
Aliyanta, B., Sumarlin O.L., Mujab S.A., 2011. Penggunaan Biokompos dalam Bioremediasi Lahan Tercemar Limbah Minyak Bumi, J. Valensi, vol. 2 (3):430-442
Pradip, K. J., C.S. K. Mishra, D.K. Behera, S. Mishra, and L.B. Sukla, 2012. Dissolution of heavy metals from electrostatic precipitator Dust a Coal Based sponge Iron By Fungal Leaching, J. African of Environmental Science and Technology, vol. 6(4): 208-213
Akinde, S.B., Iwuozor, C.C. and Obire, Omokaro, 2012. Alkane degradative potentials of bacteria isolat from the deep atlantic ocean of the Gulf of Guinea. J. Bioremed Biodegr., vol. 3 (1): 2-6
Ke Pan, On On Lee, Pei-Yuan Qian, Wen-Xiong Wang, 2011. Sponges and sediments as monitoring tools of metal contamination in the eastern coast of the Red Sea, Saudi Arabia, J. Marine Pollution, vol. 62: 1140–1146
Li, Xuanzhen, Yucheng Wu, Xiangui Lin, Jing Zhang, Jun Zeng, 2012. Dissipation of polycyclic aromatic hydrocarbons (PAHs) in soil microcosms amended with mushroom cultivation substrate. J. Soil Biology & Biochemistry, vol. 47: 191-197
Wu,Yucheng, Ying Teng, Zhengao Li, Xuewei Liao, Yongming Luo, 2008. Potential role of polycyclic aromatic hydrocarbons (PAHs) oxidation by fungal laccase in the remediation of an aged contaminated soil. J. Soil Biology & Biochemistry, vol. 40:789–796
Gan, S., E.V. Lau, H.K. Ng, 2009. Remediation of soils contaminated with polycyclic aromatic hydrocarbons (PAHs). J. of Hazardous Materials, vol. 172: 532–549
Okoh, A.L., 2006. Biodegradation alternative in the cleanup of petroleum hydrocarbon pollutants. J. Biotechnol. And Molecular Biology Review, vol. 1 (2): 38-50
Okoro, C.C., 2010. Application of seawater microbial inocula for the remediation of hydrocarbon polluted Mangrove Swamp in the Nigeria Oil Rich Niger Delta, Journal Nature and Science, vol. 8 (8): 152-162
Ijah U. J. J., and L. I. Ukpe. 2008. Biodegradation of Crude Oil by Bacillus Strain 28A and 61B Isolated from Oil Spilled Soil. J. Waste Management , vol. 12: 55-60
Romimohtarto, K & Juwana, S., 2001. Biologi Laut, Ilmu Pengetahuan Tentang Biota Laut, Pusat Penelitian dan Pengembangan Oseanologi-LIPI. Jakarta. pp. 115–128.
Syakti, D. A., Yani, M., Hidayati, V.N., Siregar, S.A. Doumeng, P., I.M. Sudiana, M., 2013. The Bioremediation potential of hydrocarbonoclastic bacteria isolated from a Mangrove Contaminated by Petroleum Hydrocarbons on the Cilacap Coast, Indonesia. J. Bioremediation, vol. 17 (1): 11–20, ISSN: 1088-9868 online. DOI: 10.1080.731446
Rivas, F. Javier, 2006. Polyciclic aromatic hydrocarbons sorbed on soils: A short review of chemical oxidation based treatments. J. of Hazardous Materials B, vol. 138: 234-251
Tapilatu Y, Acquaviva M, Guigue C, Miralles G, Bertrand JC., 2010. Isolation of alkane-degrading bacteria from deep-sea Mediterranean sediments. Letters in Applied Microbiology, vol. 50: 234-236
Okoh, A.I. 2003. Biodegradation Of Bonny Light Crude Oil In Soil Microcosm By Some Bacterial Strains Isolated From Crude Oil Flow Stations Saver Pits In Nigeria. African J. of Biotechnology, vol. 2 (5): 104-108
Tam, N.F.Y and Wong, Y.S., 2008. Effectiveness of bacterial inoculum and mangrove plants on remediation of sediment contaminated with polycyclic aromatic hydrocarbons, Marine Pollution Bulletin, vol. 57: 716-728
Lin, Y., and L. X. Cai, 2008. PAH-degrading microbial consortium and its pyrene-degrading plasmids from mangrove sediment samples in Huian, China. Marine Pollution Bulletin, vol. 57: 703–706
Avecedo, Francisca, Lecticia, Pizzul, Maria, del Pilar Castilloc, Raphael, Cuevas, Maria, Cristina Diez, 2011. Degradation of Polyciclic aromatic hydrocarbons by the Chilean white-rot fungus Anthracophyllum discolor. J. of Hazardous Materials, 185, pp. 212-219
Marzuki, I., Noor, A., Djide, N.M., La Nafie, N., 2014. Isolation and Identification on Degradator Bacterial of Petroleum waste which Symbionts with Sponge Callyspongis sp from Melawai Beach. Proceeding: The 1st International Confrence on the sciences (ICOS), ISBN : 9786027219809, pp. 493-503
Rachmaniar, R., 2007. Spons Indonesia Kawasan Timur. J. Oseanologi dan Limnologi Indonesia, vol. 3: 123-138
Marzuki, I., Noor, A., Djide, N.M., La Nafie, N., 2015. Molecular characterization of gene 16S rRNA micro symbionts in sponge at Melawai Beach, East Kalimantan. J. Marina Chimica Acta, 16 (1): 38-46
Ismet, S.M., Soedharma, D., Effendi, H., 2011. Morphology and cell biomass of sponge Aaptos aaptos and Petrosia sp. J. Ilmu dan Teknologi Kelautan Tropis. vol. 3 (2): 153-16
Meutia, S.P., Soedharma, D., Effendi, H., 2011. Morfologi dan biomassa sel spons Aaptos aaptos dan Petrosia sp. J. Ilmu dan Teknologi Kelutan Tropis, vol. 3, (2): 153-161
Gazave E, Lapébie P, E Renard, Vacelet J, C Rocher, Ereskovsky AV, Lavrov DV, Borchiellini C, 2010. Filogeni molekuler mengembalikan subdivisi supragenerik spons homoscle-romorph (Porifera, homoscleromorpha). J. PLoS One. 2010 Dec 14; 5 (12): e14290. doi : 10.1371/ journal.pone. 0014290
Ruppert, E.E., Fox, R.S., and Barnes, R.D., 2004. Invertebrate Zoology, (7 ed.). Brooks / Cole. Pp. 76-97. ISBN 0-03-025982-7
Nugroho, A., 2009. Produksi gas hasil biodegradasi minyak bumi: Kajian awal aplikasinya dalam microbial enhanced oil recovery (MEOR), J. Makara Sains, vol. 13 (2): 111-116
Nugroho, A., 2006. Biodegradasi sludge minyak bumi dalam skala makrokosmos, J. Makara Teknologi, vol. 10 (2): 82-89
Mariano, 2007. Laboratory study on the bioremediation of diesel oil contaminated soil from a petrol station. Braz. J. of Micro, vol. 38 (2): 346-353
White R J., Patel J., Ottesen A., Arce G., Blackwelder P., Lopez V. J., 2012, “Pyrosequencing of Bacterial Symbionts within Axinella corrugata Sponges: Diversity and Seasonal Variability,” www. plosone.org, e38204, J. PLoS ONE, 7(6), pp. 1-12
Teng, Ying, Yongming Luo, Mingming Sun, Zengjun Liu, Zhengao Li, Peter Christie, 2010. Effect of bioaugmentation by paracoccus sp. strain HPD-2 on the soil microbial community and removal of polycyclic aromatic hydrocarbons from an aged contaminated soil. J. Bioresource Technology. vol. 101: 3437-3443
Yani, M., dan Akbar, Y., 2012. Proses biodegradasi minyak diesel oleh campuran bakteri pendegradasi hidrokarbon, Bioscientiae, J. Tekn. Industri Pertanian, vol. 19 (1): 40-44
Liu Z, Shao Z. 2007. The diversity of alkane degrading bacteria in the enrichments with deep sea sediment of the South China Sea. J. Wei Sheng Wu Xue Bao., vol. 47: 869-873
Obire, O., Akinde, S.B., 2011. In-situ physic-chemical properties of the deep Atlantic Ocean water colum and implications on heterotrophic bacterial distribution in the guff of Guines, J. Advances in applied Science Research, vol. 2 (2): 470-482
Erdogan, Esin, and Karaca, Ayten. 2011. Bioremediation of Crude Oil Polluted Soils. Asian J. of Biotechnology, vol. 3 (3): 206-213
