ANALYSIS OF Fe, Zn, AND Cr AT SOME FRACTIONATED PROTEINS IN SPONGE AGELAS NAKAMURAI
AbstractThis research aims to analyze the concentration of metals Fe, Zn, and Cr in protein fractions of sponge Agelas nakamurai from Kapoposang Island, Spermonde archipelago. Protein samples were extracted, fractionated using various saturated ammonium sulphate (SAS) concentration, namely 0-20%, 20-40%, 40-60% and 60-80%, then purified by dialysis using celophane membranes. Each fraction was digested using microwave before metal analysis using ICP OES (Inductively Coupled Plasma Optical Emission Spectroscopy). The results have shown that highest iron concentration was found in 20-40% fraction, 28.2 µg/L, while lowest one was in 60-80% fraction, 2.39 µg/L. For Zn, its highest concentration were found 20-40% fraction, 9.42 µg/L and the lowest one was 40-60%, 3.66 µg/L. On the other hand, the concentration of Cr was found highest at 20-40% fraction, 2.28 µg/L, while its lowest amount was at 60-80% fraction, 0.238 µg/L. In the meantime, its high- est antibacterial activity was at 0-20% fraction examined in Salmonella thypusa with inhibition diameter was 7,00 mm, where the amount of essential metals Fe, Zn and Cr are 17,2 µg/L, 6,32 µg/L, 1,47 µg/L. It was concluded that the essential metals investigated were coexisted and distrib- uted in several protein fractions and its bioactive properties.Keywords : Agelas nakamurai, Bioactive protein, essential metals, ICP-OES, Kapoposang Island
Ahmad, A., Prastawa, B. dan Salama, D. 2006, Bioaktivitas antimikroba dan antikanker fraksi protein yang diisolasi dari beberapa spesies makro alga di pulau Barang Lompo Sulawesi Selatan. Prosiding Seminar Nasional Research Grant TPSDP Batch II, Bali.
Arifin, Zainal, 2008, Beberapa Unsur Mineral Esensial Mikro Dalam Sistem Biologi
De Voogd, N., J., 2005, Indonesian Sponge Biodoversity and Marine Cultured Potential, Netherland, Geborente Dodrecht, ds 174h.
Netty, A., 2014, Penggunaan callyspongia sp dari perairan Halong (Teluk Ambon) sebagai Biomonitor untuk logam runut Pb, Cd, Cr dan Zn, Jurnal Marina Chimica Acta Volume 16
Noor, A., 2014, Analisis Unsur Runut, Yayasan Mitra Sains, Makassar.
Poedjiadji, 1994, Dasar-Dasar Biokimia, UI Press, Jakarta.
Reha, W., Noor, A., Ahmad, A., La Nafie, N., Salama, D., 2013, karakterisasi Protein Aktif Dari Spons Dan Microba Simbionnya Sebagai Usaha Awal Menuju Agen Imunostimulan, Jurnal Marina Chemica Acta Volume 14(1), 1411-2132