THE POTENTIAL OF METABOLITES PRODUCED BY Lactobacillus Plantarum ATCC 8014 AS A BIOPRESERVATIVES AND ANTI-BACTERIAL Materials in ANIMAL FOOD PRODUCTS

Authors

  • Akhmad Hidayatulloh Fakultas Peternakan Universitas Padjadjaran Jl. Raya Bandung – Sumedang KM 21 Jatinangor 45363 Sumedang Jawa Barat Indonesia
  • J. Gumilar Labotorium Teknologi Pengolahan Produk Peternakan. Fakultas Peternakan Universitas Padjadjaran Jl. Raya Bandung – Sumedang KM 21 Jatinangor 45363 Sumedang Jawa Barat Indonesia
  • E. Harlia Laboratorium Mikrobiologi dan Penanganan Limbah Peternakan. Fakultas Peternakan Universitas Padjadjaran Jl. Raya Bandung – Sumedang KM 21 Jatinangor 45363 Sumedang Jawa Barat Indonesia

Keywords:

Lactobacillus plantarum, metabolites, biopreservation, anti-bacterial, pathogenic bacteria.

Abstract

Lactobacillus plantarum is a probiotic-lactic acid bacteria that normally lives in the human digestive tract. These bacteria are known to produce metabolites that have inhibitory activity against pathogenic bacteria and food spoilage. Therefore, the metabolites produced by L. plantarum was potential to be used as a biopreservation for animal product, especially milk and meat. In order to obtain accurate data about the optimal production time of these metabolites and their antibacterial activity, the growth curve of L. plantarum which was incubated up to 48 hours at 37 oC and 5.0% of CO2 was observed. Observations were also made on the inhibition of gram-negative pathogenic bacteria (Escherichia coli and Salmonella typhimurium) and gram-negative (Staphylococcus aureus and Listeria monocytogenes) which are often found in meat and milk. The results showed that the metabolites produced during the incubation process can inhibit gram-negative and gram-positive pathogenic bacteria. The best production of metabolites is at the 24th hour which shows the diameter of the inhibition zone of 11.9 mm for L. monocytogenes; 11.6 mm for E. coli; 12.5 mm for S. typhimurium and 9.1 mm for S. aureus. Therefore, the production process of biopreservation for food from the results of L. plantarum's metabolism has a potential to be developed and applied to animal product, especially milk and meat.

References

Buckle, K.A., R.A. Edward, G.H. Fleet, dan R.D. Applemen. 1987. Ilmu Pangan. Diterjemahkan oleh Hari Purnomo dan Adiono. UI Press. Jakarta.

Cheigh, C. I., Choi, H. J., Park, H., Kim, S. B., Kook, M. C.,Kim, T. S., Hwang, J. K., and Pyun, Y. R. (2002) Influence of growth conditions on the production of a nisin-like bacteriocin by Lactococcus lactis subsp. lactis A164 isolated from kimchi. J. Biotechnol. 95, 225-235.

Dalie DKD, Deschamps AM, Richarrd-Forget F. 2010. Lactic acid bacteria: potential for control of mould growth and mycotoxins: a review. Food Control 21:370-380.

Delgado, A., Brito D., Fevereiro P., Peres C., Marques J.F. 2001. Antimicrobial Activity of L.plantarum isolated from traditional lactid acid fermentation of table olives. INRA, EDP Sci. 81 (1): 2013-215

Djaafar, T.F., Rahayu, E.S., Wibowo, Sudarmadji. (1996). Substansi Antimikroba Bakteri Asam Laktat yang Diisolasi dari Makanan Hasil Fermentasi Tradisional Indonesia. Jurnal Pertanian Indonesia, 1, 15–21.

Fardiaz, S. 1992. Bakteriologi Pangan. Gramedia Pustaka Utama. Jakarta.

Frazier, W.C. and D.C. Westhoff. 1988.Food Microbiology. McGraw-Hill book Company, New York.

Gaman, P.M. dan K.B. Sherrington. 1994. Pengantar Ilmu Pangan Nutrisi dan Mikrobiologi. Gadjah Mada

Griffin, C., O’connor, P. M., Schaad, G., Bruining, C. 2011. Inhibitory activity of Lactobacillus plantarum LMG P-26358 against Listeria innocua when used as an adjunct starter in the manufacture of cheese. Microbial Cell Factories. 10, S7.

Jeevaratnam, K., Jamuna, M., dan Bawa, A.S. 2005. Biologcal Preservation of Foods-Bacteriocins of Lactic Acid Bacteria. Indian Journal of Biotechnology. Vol 4. October 2005. pp 446-454

Miwada INS, Lindawati SA, Tatang W. 2006. Tingkat efektivitas “starter” bakteri asam laktat pada proses fermentasi laktosa susu. JITAA 31(1): 32-35.

Nandi A, Dan SK, Banerjee G, Ghosh K, Ringo E, Ray AK. 2017. Probiotic Potential of autochonous bacteria isolated from the gastrointestinal tract of four freshwater teleost. Protbiotics antimicro Prot. 9(1) : 12-21.

Puspawati NN, Nuraida L, Adawiyah DR. 2010. Penggunaanberbagai jenis bahan pelindung untuk mempertahankan viabilitas bakteri asam laktat yang diisolasi dari air susu ibu pada proses pengeringan beku. J Teknol Ind Pangan. 1: 59-65.

Setioningsih, E., R.Setyaningsih,A. Susilowati. 2004. Pembuatan Minuman Probiotik dari Susu Kedelai dengan Inokulum Lactobacillus casei, Lactobacillus plantarum, dan Lactobacillus acidophillus. Bioteknologi 1 (1): 1-6, Mei 2004, ISSN: 0216-6887.

Suma, K., Misra, M. C., and Varadaraj, M. C. (1998) PlantaricinLP84, a broad spectrum heat-stable bacteriocin of Lactobacillus plantarum NCIM 2084 produced in a simple glucose broth medium. Int. J. Food Microbiol. 40, 17-25.

Tagg, J.R. and Mcgiven, A.R. (1971) Assay Systems for Bacteriocins. Applied and Environmental Microbiology, 21, 943-947.

Yuliana N. 2008. Kinetika pertumbuhan bakteri asam laktat isolat T5 yang berasal dari tempoyak. J Teknol Ind Hasil Pert. 13(2): 108-116

Zwietering M H, Jongenburger, I, Rombouts F M, et al.1990, Modeling of the bacterial growth curve. Applied and Environmental Microbiology, vol.56(6), 1875−1881

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2019-07-26

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