TOTAL BAKTERI ASAM LAKTAT DAN COLIFORM USUS ITIK CIHATEUP YANG DIBERI PAKAN MENGANDUNG KOMBINASI TEPUNG KULIT MANGGIS DAN KUNYIT (Total Lactic Acid Bacteria and Coliform of Cihateup Ducks Intestine that Given Feed Containing the Combination of Mangosteen Peel and Turmeric Flour)

Authors

  • Andri Kusmayadi Program Studi Peternakan, Fakultas Pertanian, Universitas Perjuangan Jalan Pembela Tanah Air No. 177, Tasikmalaya 46115, Jawa Barat, Indonesia
  • Novia Rahayu Program Studi Peternakan, Fakultas Pertanian, Universitas Perjuangan Jalan Pembela Tanah Air No. 177, Tasikmalaya 46115, Jawa Barat, Indonesia

DOI:

https://doi.org/10.20956/jitp.v8i1.8197

Keywords:

lactic acid bacteria, coliform, Cihateup duck, mangosteen, turmeric

Abstract

The number of beneficial microbial populations (lactic acid bacteria/LAB) and pathogens (coliform) in the digestive tract of ducks is one indicator of duck health status. Healthy ducks have a beneficial microbial population more than pathogenic bacteria. The addition of herbal ingredients such as mangosteen peel and turmeric flour is thought to improve the health of ducks by increasing the LAB population. The purpose of this study was to examine the effect of the combination of mangosteen peel flour and turmeric flour on the total lactic acid bacteria and coliform of small intestine and caecum of Cihateup duck. The study was conducted experimentally using 60 male Cihateup ducks grouped into 5 feed treatments which contains a combination of mangosteen peel and turmeric flour at different levels as follows: 0.5% (R1); 1.0% (R2); 1.5% (R3); 2.0% (R4); and 2.5% (R5), respectively. The variables studied were total lactic acid and coliform bacteria in the small intestine and caecum of Cihateup ducks. The results showed that the treatments of feed significantly (P<0.05) affected the total lactic acid bacteria (except caecum) and coliform bacteria. The population of lactic acid bacteria tended to increase along with the increasing dose of combination treatment of mangosteen peel and turmeric flour. In contrast, the total population of coliform bacteria in the small intestine and caecum of ducks showed a tendency to decrease with increasing treatment doses. The use of a combination of mangosteen peel and turmeric flour at higher levels (2.0 – 2.5%) can increase the total LAB and decrease coliform bacteria in the small intestine and cecum of Cihateup duck.

References

Saepudin, A., D. Natawijaya, E. Hartini and R .Iskandar. 2019. Evaluation of antibacterial activity of mangosteen (Garcinia mangostana L.) pericarp extract against rice leaf blight bacteria (Xanthomonas oryzae pv. oryzae) at various temperatures and durations of fruit storage. IOP Conf. Ser.: Earth Environ. Sci., 250 012026

Al-Mashhadani, H.E. 2015. Effect of different levels of turmeric (Curcuma longa)

supplementation on broiler performance, carcass characteristic and bacterial count. Egypt. Poult. Sci., 35(1): 25-39.

Amalia, S., Wahdaningsih, S., Untari E.K. 2014. Antibacterial activity test of fraction n-hexane of red dragon fruit skin (Hylocereus polyrhizus) against bacteria Staphylococcus aureus ATCC 25923. Trad. Med. J., 19(2): 89-94.

Basak, D.P., T. Adhikary, P. Das, and S. Biswas. 2018. Phytochemical analysis and comparative study of antibacterial effect of turmeric extracts using different solvent. IOP Conf.Series: Mat. Sci. Eng. 410 012018. doi:10.1088/1757-899X/410/1/012018

Christaki, E., E. Bonos, I. Giannenas and P. Florou-Paneri. 2012. Aromatic plants as a source of bioactive compounds. Agriculture, 2(4): 228–243.

Fatmala, R. 2015. Pengaruh konsentrasi ekstrak etanol kulit manggis (Garcinia mangostana Linn) terhadap daya hambat pertumbuhan bakteri Lactobacillus acidophilus (Kajian In Vitro). Skripsi. Fakultas Kedokteran Gigi, Universitas Muhammadiyah Surakarta, Surakarta.

Feghali, P.A.R.E., R. Ibrahim, T. Nawas. 2018. Antibacterial activity of curcumin against Lebanese clinical isolates of Staphylococcus aureus. MOJ Toxicol., 4(2): 81-83.

Gondokesumo, M.E., B. Pardjianto, S.B. Sumitro, W. Widowati, and K. Handono. 2019. Xanthones analysis and antioxidant activity analysis (Applying ESR) of six different maturity levels of mangosteen rind extract (Garcinia mangostana Linn.). Pharmacog J., 11(2): 369-373.

Gupta, A., S. Mahajan and R. N. Sharma. 2015. Evaluation of antimicrobial activity of Curcuma longa rhizome extract against Staphylococcus aureus. Biotechnology Reports., 6: 51-55.

Halimatunnisroh, R., T. Yudiarti, and Sugiharto. 2017. Total coliform, acid bacteria and total bacteria in intestine of broiler chicken given turmeric. Jurnal Peternakan Indonesia, 19(2): 79-84.

Kusmayadi, A., K.R. Bachtiar, and C.H. Prayitno. 2019. The effects of mangosteen peel (Garcinia mangostana L.) and Turmeric (Curcuma domestica Val) flour dietary supplementation on the growth performance, lipid profile, and abdominal fat content in Cihateup ducks. Veterinary World, 12(3): 402-408.

Melkianus, B., Fatimawali, dan S. Sudewi. 2019. Uji aktivitas antibakteri ekstrak kulit buah manggis (Garcinia mangostana L.) terhadap bakteri Klebsiella pneumonlae. PHARMACON Jurnal Ilmiah Farmasi, 8(1): 88 – 93.

Namagirilakshmi, S., P. Selvaraj, K. Nanjappan, S. Jayachandran and P. Visha. 2010. Turmeric (Curcuma longa) as an alternative to in-feed antibiotic on the gut health of broiler chicken. Tamilnadu Journal of Veterinary and Animal Sciences, 6(3): 148-150.

Natsir, M.H., E. Widodo, dan Muharlien. 2016. Penggunaan kombinasi tepung kunyit (Curcuma domestica) dan jahe (Zingiber officinale) bentuk enkapsulasi dan tanpa enkapsulasi terhadap karakteristik usus ayam pedaging. Buletin Peternakan, 40(1): 1-10.

Ngajow, M., J. Abidjulu and V.S. Kamu. 2013. Antibacterial effect of stem matoa (Pometia pinnata) skin extract on Staphylococcus aureus bacteria in vitro. Jurnal MIPA Unsrat Online, 2(2): 128-132.

Poeloengan, M. dan Praptiwi. 2010. Antibacterial activity test of mangosteen peel extract (Garcinia mangostana Linn). Media Litbang Kesehatan, 20(2): 65-69.

Putra, INK. 2010. Antibacterial activity of mangosteen peel extract (Garcinia mangostana L.) and its active compound content. J. Teknol. Dan Industri Pangan, 21:1-5.

Raheem, S.M.A., S.M.S.A. Allah dan K.M.A. Hassanein. 2012. The effects of prebiotic, probiotic and symbiotic supplementation on intestinal microbial ecology and histomorphology of broiler chickens. IJAVMS, 6(4): 277-289.

Rahmah, A., N. Suthama, V.D. Yunianto. 2013. Total lactic acid bacteria and Eschericia coli in broiler chickens given dietary herbal mixed. Animal Agriculture Journal, 2(3): 39-47.

Sabir, A. 2005. Antibacterial activity of flavonoids propolis Trigona sp. against Streptococcus mutans bacteria (in vitro). Maj. Ked. Gigi. (Dent. J.), 38(3):135–141.

Smith, H.W. 1965. Observations on the flora of the alimentary tract of animals and factors affecting its composition. J. Pathol Bacteriol., 89: 95-122.

Tabbu, C.R., 2000. Penyakit ayam dan penanggulangannya. Penyakit mikal, bakterial,

viral. Kanisius, Yogyakarta.

Warni, I.S. 2018. Pengaruh pemberian air rebusan kunyit (Curcuma domestica) terhadap total mikroba dan Escherichia coli serta bobot sekum ayam broiler. Skripsi. Program Studi Peternakan, Fakultas Peternakan dan Pertanian, Universitas Diponegoro, Semarang.

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Published

2020-01-23

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