Synthesis and Characterization Gold Nanoparticles Using Extracts of Sarang Semut (Myrmecodia pendans) For Sensing of Glucose

Andi Suciati, Abdul Wahid Wahab, Paulina Taba


Abstract. Gold nanoparticles have been synthesized with simple and relatively safe method by using kaemferol and quercetin containing aqueous extract of Myrmecodia pendans (sarang semut plant). Under this method, it was able to obtain AuNPs and measured the size. The formation of the nanoparticles were identified by the color change (yellow to red) occurred in the chloroauric acid solution after additional aquoeos extract of sarang semut plant. The UV-Visible spectra and particle size analyzer indicated the successful formation of AuNPs with wavelength of 521.5 nm which was stable for 4 days, evidenced by the presence of particles with a size 53.2 nm. XRD analysis showed the difractogram peak of gold nanopartcle. The result of SEM analysis indicated gold nanopartcle surface with spherical and rod-like shapes. Glucose sensor utilized gold nanopartcle as catalyst with measurement range 2–5 mM and regresision 0,868, detection limit of sensor was in concentration 2-5 mM  was 7,5 mM with sensitivity 0,736 Glucose concentration obtained in blood serum was 78,48mg/dL. Gold nanoparticles is promising to detect blood sugar with simply, securely, low cost without using enzyme and harmful material.


biosynthesis, gold nanoparticles, sarang semut

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Ahmad, T., Irfan, M., Azmi, M. B., Bhattacharjee, S. 2016. Effect of Reaction Time on Green Synthesis of Gold Nanoparticles by Using Aqueous Extract of Elaise Guineensis (Oil palm leaves).Procedia Engineering. 148: 467 – 472

Ahmed, S. dan Ikram, S. 2015. Synthesis of Gold Nanoparticles using Plant Extract.An Overview. 1(1):5

Amanda S. Barnard dan Larry A. Curtiss. 2006. Predicting the shape and structure of face-centered cubic gold nanocrystals smaller than 3 nm. ChemPhysChem. 7, 1544 – 1553.

Basavegowda, N., Idhayadhulla, A., Lee, Y.R. 2014.Phyto-Synthesis of Gold Nanoparticles Using Fruit Extract of Hovenia Dulcis and Their Biological Activities.Ind. Crops Prod. (52): 745–751.

Beltrame, P., Comotti, M., Pina, C. D., Rossi, M. 2006.Aerobic Octidation of Glucose II.Catalysis by Colloidal Gold, Appl. Catal. A: Gen. 297: 1-7.

Commoti, M., Pina, C.D., Matterese, R., Rossi, M. 2004. The Catalytic activity of “naked” gold particles , Angew. Chem. Int. 43: 5812-5815.

Davey, W. P. 1925. Precision Measurements Of The Lattice Constants Of Twelve Common Metals. Physical Review. 25: 753-761

Engida, A. M., Kasim, N. S., Tsigie, Y. A., Ismadji, S., Huynh, L. H., Yi-Hsu, J., 2013. Extraction, Identification and Quantitative HPLC Analysis of Flavonoids from Sarang Semut (Myrmecodia pendan).Industrial Crops and Product, 41: 392-396.

Fachrurrazie, T. A.I dan Wibowo, W., 2017.Preparation of Glucose Sensors using Gold Nanoparticles Modified Diamond Electrode.Materisal Science and Engineering.

Gao ,Y., Wu, Y., Di, J. 2017. Colorimetric Detection of Glucose Based on Gold Nanoparticles Coupled With Silver Nanoparticles. Chemical Engineering and Material Science.Elsevier.

Fadhilah, R. 2013. Biosensor Glukosa Menggunakan GDH-FAD yang Diimobilisasi pada Nanopartikel Zeolit sebagai Elektrokimia. Intstitut Pertanian Bogor.

Fernandez, B. R. 2011. Sintesis Nanopartikel. Makalah diterbitkan.Program Studi Kimia Pascasarjana Universitas Andalas Padang.

Iravani, S., Korbekandi, H., Mirmohammadi, S.V., Zolfaghari, B. 2014. Synthesis of Silver Nanoparticles: Chemical, Physical and Biological Methods, Res. Pharmaceutical Sci. 9: 385–406.

Karra S., Wooten M., Griffith W., Gorski W. 2016. Morphology of Gold Nanoparticles and Electrocatalysis of Glucose Oxidation.Electrochimica Acta.

Kurniawan, F., Tsakova, V., Mirsky, M. V. 2006.Gold Nanoparticles in Nonenzymatic Electrochemical Detection of Sugars.Electroanalysis. 18: 19-20.

Kurniawati, E., Sianturi, Y.C. 2016.Manfaat Sarang Semut (Myrmecodiapendans) sebagai Terapi Antidiabetes.Majority.5(3).

Luo, W. J., Zhu, C. F. Su, S. Li, D., He, Y., Huang, Q., Fan, C. H. 2010. Self- Catalyzed, Self- Limiting Growth of Glucose Oxidase-Mimicking Gold Nanoparticles. ACS Nano 4: 7451-7458.

Mukunthan, K. dan Balaji, S. 2012. Cashew Apple Juice (Anacardium Occidentale L.) Speeds Up The 778 Synthesis of Silver Nanoparticles.Int J. Green Nanotechnol.4(2).

Nagaraj, B., Malakar, B., Divya, T., Krishnamurthy, N., Liny, P., Dinesh, R. 2012. Environmental Benign Synthesis of Gold Nanoparticles From The Flower Extracts of Plumeria Alba Linn,(Frangipani) and Evaluation of Their Biological Activities. Int. J. Drug Dev Res. 4(1):144-150.

Nath, D. dan Banerjee, P. 2013. Green Nanotechnology - A New Hope For Medical Biology, Environ. Toxicol.Pharmacol. 36: 997-1014.

Noruzi, M. 2015. Biosynthesis of gold nanoparticles using plant extracts. Bioprocess Biosyst Eng. 38:1–14

Pal, N.B., Kryschi, C,. 2015. A Facile One-Pot Synthesis of Blue and Red Luminescent Thiol Stabilized Gold Nanoclusters: A Thorough Optical And Microscopy Study. Phys. Chem. 17, 21423-21431.

Pasta, M., La Mantia, F., dan Cui, Y., 2010, Mechanism of Glucose Electrochemical Oxidation on Gold Surface, Electrochimica Acta, 55: 5561-5568.

Philip, D. 2010. Low-dimensional Systems and Nanostructures. Physica E. 42(5): 1417.

Raghunandan D, Bedre MD, Basavaraja S, Sawle B, Manjunath SY. 2010. Rapid Biosynthesis of Irregular Shaped Gold Nanoparticles from Macerated Aqueous Extracellular Dried Clove Buds (Syzygium Aromaticum) Solution. Colloids Surfaces B Biointerfaces. 79: 235-240.

Satoshi Horikoshi and Nick Serpone. 2013. Microwaves in Nanoparticle Synthesis, First Edition. Wiley-VCH Verlag GmbH & Co. KGaA.

Vijayaraghavan, K., dan Ashokkumar, T. 2017. Plant-Mediated Biosynthesis of Metallic Nanoparticles: A Review of Literature, Factors Affecting Synthesis, Characterization Techniques and Applications. J. Env. Chem. Eng. 5:4866.

Wang, J. 2000. Analytical Electrochemistry, Edisi 2. New York: John Wiley and Sons, Inc.

Wulandari, M. 2011. Teknik Imobilisasi Adsorpsi dan Entrapment Film Nata De coco- Benedict untuk Deteksi Kadar Gula dalam Urin.ISSN 1979-8911.5(1-2)

Zhou, Y., Lin, W., Huang, J., Wang, W., , Y., Lin, L., Li, Q. dan Du, M. 2010. Biosynthesis of Gold Nanoparticles by Foliar Broths: Roles of Biocompounds and Other Attributes of the Extracts. Nanoscale Res. Lett.5(8) :1351.



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