POTENSI ABALON TROPIS Haliotis asinina L. SEBAGAI SUMBER INOKULUM JAMUR SIMBION PENGHASIL ANTIMIKROBA

Authors

  • Magdalena Litaay
  • Karlina Sari
  • Risco B Gobel
  • Nur Haedar

Abstract

The research about “The Potencial of Tropical Abalone Haliotis asinina L. As Source of Mushroom Antimicroba Producing

Symbionts” had been done. This research aimed to know the abalone potency as a source of inoculum and to characterize

isolate fungal symbionts H. asinina L. Isolation of fungi symbionts H. asinina L. was performed used a PDA medium (Potato

Dextrose Agar). Characterization of isolates fungal symbiont from H. asinina L. consists of macroscopic and microscopic

observations, and activity testing against pathogenic bacteria and fungi. The results showed that there were isolates of fungal

symbionts H. asinina L. (Abl.J.1, Abl.J.2, and Abl.J.3). The results of macroscopic observation colony indicated Abl.J.1 and

Abl.J.3 isolate had a surface likes flour and Abl.J.2 isolate had a flat surface such as cotton; Abl.J.1 isolate green, Abl.J.2 isolate

light green and Abl.J.3 isolate black in colours. Three isolates had concentric circles; isolates Abl.J.1 and Abl.J.3 had radial

lines and isolate Abl.J.2 had not radial line. The result of microscopic observation showed that three isolates had not septa,

and hyaline (colorless); three isolates had asexual spores conidioshpore and all isolates was suspected to belong to the genus

Aspergillus. All isolates were able to inhibit the growth of Salmonella thypi bacteria and Candida albicans fungus and the

resulting compounds were bacteriocidal and fungicidal.

Keyword: Gastropods, H. asinina L., Symbiont fungus, Antimicrobal, Aspergillus.

References

Balcázar, J. L., I. B Ruiz-Zarzuela, D. Cunningham, D.

Vendrell, and J.L. Múzquiz. 2006. The role of

probiotics in aquaculture. Veterinary Microbiol.

: 173-186.

Barnet, H. L and B. B. Hunter. 2000. Illustrated

Genera of Imperfect Fungi. Burgess Publishing

Company. USA. 241 pp.

Blunt, J. W., B. R. Copp, M. H. G. Munro, P. T.

Northcote, and M. R. Prinsep. 2006. Marine

natural products. Natural Product Reports,

:26–78.

Bugni T. S, and C. M, Ireland. 2004. Marine-derived

fungi: A Chemically and Biologically Diverse

Group of Microorganisms. Nat. Prod. Rep.,

:143-63.

Burgessa, J. G., K. G. Boyda, E. Amstronga, Z. Jianga,

L. Yana, M. Berggrenb, U. Mayb, T. Pisacanec,

A. K. Granmob, and D. R. Adamsd. 2003. The

Development of a Marine Natural Product-based

Antifouling Paint. Biofouling, 2003. 19:197-

Campbell, N. A., B. R. Jane, and G. M. Lawrence.

Biologi. Edisi kelima Jilid III. Erlangga.

Jakarta. 436 hal.

Cappucino, J. G. and S. Natalia. 2001. Microbiology:

A Laboratory Manual, 6th Edition. Sinaeur

Associates, Inc. Sunderland.

Dwidjoseputro, 1998. Dasar-Dasar Mikrobiologi.

Djambatan. Jakarta. 214 hlm.

Gandjar, I., R. A. Samson, A. Oetari, dan I. Santoso.,

Pengenalan Kapang Tropik Umum.

Yayasan Obor Indonesia. Jakarta.136 hlm.

Geiger, D. L, 2005. Molecular Phylogeni and The

Geograpic Orogin of Haliotidae Traced by

Haemocyanin Sequences, Journal of Molluscan

Studies Advance. Santa Barbara Museum of

Natural History. pp. 1-6.

Harvell C. D., C. E. Mitchell, J. R. Ward, S. Altizer, A.

P. Dobson, R. S. Ostfeld, M. D. Samuel. 2002.

Climate warming and disease risks for terrestrial

and marine biota. Science 296: 2158–2162.

Johanne E, M. Litaay, Syahribulan. 2016. The bioactivity

of hexadecanoic acid compound isolated from

hydroid Aglaophenia cupressina lamoureoux

as antibacterial agent against salmonella typhi.

International J. Biological Medical Research Vol

No.2.

Noverita, D. F. dan S. Ernawati. 2009. Isolasi dan Uji

Aktivitas Antibakteri Jamur Endofit dari Daun

dan Rimpang Zingiber ottensii Val. Jurnal

Farmasi Indonesia Vol. 4 No. 4 : 171-176.

Universitas Nasional. Jakarta Selatan.

Pankey, G.A. and L.D. Sabath. 2004. Clinical relevance

of bacteriostatic versus bactericidal mechanisms

of action in the treatment of Gram-positive

bacterial infection. Oxford Journal 38: 864--870.

Molinski, T.F, D.S Dalisay, S.L Lievens, J.P.Saludes.

Drug development from marine natural

products. Nature Review Drug Discovery 8:69–

Nurfadillah, A., M. Litaay, R.B.Gobel, N. Haedar.

Potensi tunikata Polycarpa aurata sebagai

sumber inokulum jamur endosimbion penghasil

antimikroba. J. Alam dan Lingkungan 6 (12):

-16.

Radjasa, O.K., Y.M. Vaske, G. Navarro, H.C. Vervoort,

K.Tenney, R.G. Linington, P. Crews. 2011.

Highlights of marine invertebrate-derived

biosynthetic products: Their biomedical

potential and possible production by microbial

associants Bioorganic & Medicinal Chemistry

: 6658–6674

Rasyid, A. 2008. Biota Laut Sebagai Sumber Obat-

Obatan. Oseana, Vol : XXXIII No. 1, Hal : 12.

Pusat Penelitian Oseanografi-LIPI. Jakarta.

Samson, R. A., E.S. Hoekstra and J.C. Frisvad.

Introduction to food and airborne fungi.

Centraalbureau Voor Schimmelcultures,

Utrecht: 383 hlm.

Singh I. P and S. B. Bharate. 2005. Anti-HIV Natural

Products. Journal Current Science. 89: 269-

Tahir E., M. Litaay, R.B. Gobel. Nur Haedar, D.

Priosambodo dan Syahribulan. 2016. Potensi

tunikata Rhopalaea sp. sebagai sumber

inokulum jamur simbion penghasil antimikroba.

J. Spermonde 2(2): 33-37.

Varoglu, M., T. H. Corbett, F. A. Valeriote and P.

Crews. 1997. Journal Org Chem. 62. 7078.

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2017-07-26

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