BAKTERI ASSOSIASI DI KARANG BATU (Skleractinian) YANG TERINFEKSI PENYAKIT TUMOR (Growth Anomalies) YANG BERASAL DARI PULAU SALEMO KABUPATEN PANGKEP

Arniati Massinai, Akbar Tahir, Jamaluddin Jompa, Alexander Rantetondok

Abstract


 Coral disease is one of the threats to the health of coral but is not yet widely known. This study was conducted to

determine the types of bacterial associations in hard corals infected with growth anomaly (GA). Coral samples

infected with GA disease were taken by using SCUBA tool on Salemo Island, Mattiro Bombang Village, Pangkep

District. Bacterial isolation was performed by removing mucus from corals infected by growth anomaly by 1

ml and diluted to dilution 10-3 . Purification of bacteria is done by inoculating the solid medium of Marine Agar.

Furthermore, Gram staining and biochemical tests were performed. Bacterial identification was performed based

on colony morphology and biochemical reaction test results. The results showed that GA disease can infect

coral Porites, Acropora digitate, Platygyra and pocillopora . Bacteria are found from the genus Flavobacterium,

Acinetobacter  and, Neiseria.

 Keywords: Bacteria association, coral stone, coral disease, growth anomaly


Full Text:

PDF

References


Acropora palmata. Mar. Biol. 77, 221–227.

Aeby, G.S., Williams, G.J., Franklin, Haapkyla, J.,

Harvell, Neale, C.D.S, Page, C.A., Raymundo,

L., Vargas-Angel, B., Willis, B.L., Work,

T.M., Davy S.K. 2011. Growth Anomalies on

the Coral Genera Acropora and Porites Are Strongly Associated with Host Density and

Human Population Size across the Indo-Pacific.

PLoS ONE 6 (2): e16887. doi:10.1371/journal.

pone.0016887

Anwar, M.A. and Choi, S. 2014. Gram Negative Bacteri:

Structure Features of Lipopolysaccarides and

Their Relevance for Economically Important

Diseases (review). Marine drugs 12: 2485-2514.

Arotsker, L. Kramarsky-winter, E., Ben-Dov, E.,

Kushmaro, A. 2016. Microbial transcriptome

profiling of black band disease in Faviid Coral

During a seasonal disease peak. Dis Aquat Org

:77-89

Austin, B. and Austin, D.A. 1993. Bacterial Fish

Pathogens: Disease in Farmed and Wild Fish.

Second edition. Taylor & Francis. London. 356 p

Autin, B. 1988. Marine Microbiology. Cambridge

University Press. Cambridge. 222 p

Bak, R.P.M., 1983. Neoplasia, regeneration and

growth in the reef building coral Acropora

palmata. Mar. Biol. 77 (3): 221-227

Barnes, M.E. and Brown, M.L. 2011. A Review of

Flavobacterium Psychrophilum Biology,

Clinical Signs, and Bacterial Cold Water

Disease Prevention and Treatment. The Open

Fish Science Journal 4: 1- 9

Baumann, P. 1968. Isolation of Acinetobacter from Soil

and Water. Journal of Bacteriology 96 (1): 39-

Bauvet, P.J.M. and Grimont, P.A.D. 1986. Taxonomy of

the Genus Acinetobacter with the Recognition of

Acinetobacter baumannii sp.nov., Acinetobacter

haemolyticus sp. Nov., Acinetobacter johnonii

sp.nov., Acinetobacter junii sp.nov., and Emended

Description of Acinetobacter calcoaceticus and

Acinetobacter iwoffii. International Journal of

Systematic Bacteriology 36 (2): 228-240.

Beeden, R., Willis, B.L., Raymundo, L.J., Page,

C.A.,and Weil, E. 2008. Underwater Cards for

Assessing Coral Health on Indo-Pacific Reef.

CRTR Program Project Executing Agency,

Center for Marine Studies. The University of

Queensland. Australia

Beleneva, I.A. and Maslennikova, E.F. 2004. Spread

of Bacteria of the genus Acinetobacter in the

Hydrobios of the Bay of Peter the Great (the Sea

of Japan). Micribiol Epidemiol Immunobiol 3:

-90

Breed, R.S, Murray, E.G.D., and Smith, N.R. 1957.

Bergey’s ManualDeterminative Bacteriology.

Seventh Edition.The Williams & Wilkins

Company. United stated of America

Burns, J.H.R, Takabayashi, M. 2011. Histopathology

of Growth Anomaly Affecting the Coral,

Montipora capitata: Implications on Biological

Functions and Population Viability. PLoS ONE

Cappuccino, J.G. and Sherman, N. 1987. Mikrobiology:

A Laboratory Manual. The Benjamin/Cummings

Publishing Company. Menlo Park. Calofornia

Cervino, J.M., Thompson, F.L., Gomes-Gil, B.,

Lorence, E.A., Goreau, T.J., Hayes, T.J.,

Winiarski-Cervino, K.B., Smith, G.W., Hughen,

K., Bartels, E. 2008. The Vibrio Core Group

Indiced Yellow Band disease in Caribbean and

indo-Pacific Reef-Building Corals. Journal

applied Microbiology. 1-17

Dublier, N., Giere, O., Distel, D.L., Mavanaugh, C.M.

Characterization of Chemoautotrophic

Bacterial Symbionts in a Gutless Marine

Worm (Oligochaeta, annelida) by Phylogenetic

S rRNA Squence Analysis and in Situ

Hybridization. Applied environmental

Microbiology 61 (6):2346-2350.

Durborow, R.M., Thune, R.L.,Hawke J. P and Camus,

A.C. 1998. Columnaris Disease A Bacterial

Infection Caused by Flavobacterium colu mnare.

SRAC Publication No. 479

Goldberg, J. and Wilkinson, C. 2004. Global Threats

to Coral Reef: Bleaching, Climate change,

Disease, Predator Plagues, and Invasive

Spesies. GCRMN. 25 p

Harvell, C.D. 2007. Coral Disease Environmental

Drivers, and The Balance Between Coral and

Microbial Associates. Oceanography 20 (1).

Harvell, C.D., Aronson, A., Baron, N., Connell, J.,

Dobson, D., Ellner, S., Gerber, L., Kim, K.,

Kuris, A., McCallum, H., Lafferty, K., McKay,

B., Porter, J., Pascual, M., Smith, G., Sutherland,

K., Ward, J., 2004. The rising tide of ocean

diseases: unsolved problems and research

priorities.. Review Front Ecol Environ 2(7):

–382

Holt, J.G., Krieg, N.R., Sneath, P.H.A., Staley, J.T

and Williams, S.T. 1994. Bergey’s Manual

of Determinative Bacteriology Ninth eds.

Lippincott Williams and Wilkis. Baltimor, USA

Hernandez-Castro, R., Martinez-Chavarria, L., Diaz-

Avelar, A., Romeo-Osorio,A., Godinez-Reyes,

C., Zovala-Gonzalez, A., Verdugo-Rodriguez,

A. 2005. Aerobic Bacterial Flora of the Nasal

Cavity in Gulf of california Sea Lion (Zalophus

californianus). Vet. J 170 (3): 359-363.

Jones, R. J., Bowyer, J. C., Hoegh-Guldberg, I.O.

and Blackall, L. L. 2004. Dynamics of a

temperature-related coral disease outbreak.

Marine Ecology Progress Series, 281 : 63-77.

Kalimutho, M., Ahmadi, A.,Kassim, Z. 2007. Isolation,

Characterization and Identification of Bacteria

Associated with Mucus of Acropora cervicornis

Coral from Bidong Island, Terengganu,

Malaysia. Malaysia Journal of Science 26 (2):

- 39

Levinson, W. 2008. Review of Medical Microbiology

& Immunology, Tenth Edition. New York: The

McGraw-Hill Companies, Inc Durborow, R.M.,

Thune, R.L.,Howke, J.P. dan Camus, A.C. 1998.

Culumnaris Disease a Bacterial Infection Caused

by Flavobacterium columnae. SRAC No 479

Liu, G., Tang, C. M., Exley, R. M. 2015. Non-

Pathogenic Neisseria: Members of an Abundant,

Multi-Habitat, Diverse genus. Microbiology

:1297-1312.

Mac Faddin, J F . 1983. Biochemical Test For

Identification Of Medical Bacteria .Second

Edition . Williams and Wilkins . Baltimore

London.

Massinai, A. 2012. Kondisi dan Sebaran Penyakit pada

Karang Batu (Stony coral) di Kepulauan Spermonde

Sulawesi Selatan. Tesis. Program Pasca Sarjana.

Universitas Hasanuddin. Makassar. 186 hal

Peters, E.C. 1997. Diseases of Coral-Reef Organisms. In:

Birkeland C (ed) Life and Death of Coral Reefs.

Chapman & Hall, New York.

Reimer, A. 2017. Search for Novel Antimicrobials Against

Neisseria Gonorrhoeae and Chlamydia Trachomatis.

Dissertation. Fach Biologie eingereicht an der

Fakultät für Biologie der Julius-Maximilians-

Universität Würzburg. Würzburg

Sidharta, B.R. 2000. Pengantar Mikrobiologi Kelautan.

Universitas Atmajaya Yokyakarta. 122 hal

Sokolow, S. 2009. Effects of a changing climate on the

dynamics of coral infectious disease: a review of the

evidence. Dis Aqua org 87: 5–18

Soslau,G. , Jacob A. , Russell, James R., Spotila, Andrew J.

M., Bagsiyao, P. 2011. Acinetobacter sp.HM746599

Isolated from Leather Back Turtle blood.

Research Letter. published online 27 July 2011.

DOI:10.1111/j.1574-6968.

Spencer, J.M., Pike, J., Munn, C.B. 2007. Diseases affect

Cold – Water Corals too: Eunicella verrucosa

(Cnidaria:Gorgonacea) Necrosis in SW England.

Diseases of Aquatic Oraganisms. Marine Instiutute,

University of Plymouth. UK. 78:87-97.Work,

T.M.,Rameyer,R.A 2005 Characterizing Lesions in

Corals From American Samoa. Coral Reefs 24:384–

Squires, D.F., 1965. Neoplasia in a coral?. Science 148: 503–

Sunagawa S., DeSantis, T., Piceno, Y. M., Brodio, E.L.,

Desavo, M.K., Vooltra, C.R., Weil, E., Anderson,

G.L. 2009. Bacterial Diversity and white Plaque

Disease Associated Community Change in The

Caribbean Coral Montastraea. The Ismejournal 3:

-521.

Sutherland, K.P., Porter, J.W., Torres, C. 2004. Disease

and Immunity in Caribbean and Indo-Paci

Wczooxanthellate Corals. Mar. Ecol. Prog. Ser. 266:

–302.

Takabayashil, M., Gregg, T.M., Farah, E., Burns, J., Teves, K.,

Cody N.H. 2008. The Prevalence of Skeletal Growth

Anomaly And other Afflictions in Scleractinian

Corals at Wai‘opae, Hawai‘I. Proceedings of the 11th

International Coral Reef Symposium, Ft. Lauderdale,

Florida, 7-11 July 2008. Session number 18

Umezawa H, Okami Y, Kurasawa S, Ohnuki T, Ishizuka

M, Takeuchi T, Shiio T, Yugari Y. 1983. Marinactan,

Antitumor Polysaccharide Produced by Marine

Bacteria. Jurnal Antibiotik 36 (5): 471-477. PMID:

Uzair B, Ahmed N. A, Mohammad F. V., Ahmad, V.U., and

Edwards, D. 2009. Screening of Marine Bacteria of

Pakistan Coast for Drug Discovery Potential. Proc.

Pakistan Acad. Sci. 46(3):137-144.

Veron, J. 2000. Coral of The World. Australian Institute

Marine Science. Townnsvile Australia. 429

halaman.

Waluyo, L. 2009. Teknik dan Metode Dasar

Mikrobiologi. Universitas Muhammadiyah.

Malang Press. Malang

Weil, E. and Hooten, A.J. 2008. Underwater Cards for

Assessing Coral Health on Caribbean Reefs.

CRTR Program Project Executing Agency,

Center for Marine Studies. The University of

Queensland. Australia

Weil, E., Smith, G., Gil-Agudelo, D.L. 2006. Status and

Progress in Coral Reef Disease Research. Dis

Aquat Org 69:1-7

Willis, B.L.,Page, C.A., Dinsdale, E,A. 2004. Coral

Diseaseon the Great Barrier Reef In: Rosenberg

E, Loya Y (eds) Coral Disease and Health. pp

-10

Work, T.M.,Rameyer, R.A. 2005. Characterizing

Lesions in Corals From American Samoa. Coral

Rees 24:384–390

Work, T.M., Aeby, G.S., Coles, S.L. 2008. Distribution

and Morphology of Growth Anomalies in

Acropora from the Indo-Pacific. Diseases

Aquatic Organisms 78:255-264

Yulianto, K. dan Mira, S. 2009. Budidaya Makroalga

(Kappaphycus alvarezii) (DOTY) secara vertical

dan gejala penyakit Ice-ice di Perairan Pulau

Pari. Oceanologi dan Limnologi Indonesia 35

(3): 323-332


Refbacks

  • There are currently no refbacks.