Effects of Kapok Seed Extract(Ceiba pentandra Gaertn) as Contraceptive Agent to the Quality of the Spermatozoa in Domestic Cat (Felis domestica)

Sitti Mughniati, Dwi Kesuma Sari, Dedy Rendrawan, Lellah Rahim

Abstract


Contraception is a way to prevent the fusion of mature egg cell and sperm during the intercourse, so the fertilization will not occur. Some contraceptive methods in small animals have been evolved. This research aimed to determine the effects of kapok seed (Ceiba pentandra Gaertn) extract as contraception agent to the quality of spermatozoain domestic cats. The animals were divided into four group with each group consisted of six male cats. Group I, II, III was designated as the treatment groups and IV as the control group. The cats were given the emulsion of kapok seeds extract orally with concentration of 0,1%  b/v, 0,71 mg/kg BB, 0,36 mg/kg BB and 0,21 mg/kg BB doses. This study used Randomized Complete Random Design (RAL) design from sperm quality data and used ANOVA statistic test. The result showed that the concentration of sperm decreased in the treatment groups compared with the control group. Statistically, the sperm concentration decreased significantly (P < 0,05) between the control group and treatment groups. The average motility in low and medium doses was above 40% while it was below than 40% in the high doses group. Based on the results, the granting of kapok seed extract inhibited the viability of spermatozoa of cats with significant results (P < 0,05). Sperm abnormalities in this study was obtained from the control group with percentage average of 1 % , while it was significantly increased in the treatment groups marked by abnormalities in the shape of the head and tail of the spermatozoa. These results suggested that kapok seed extract can be used as one of the contraceptive agents in domestic cat (Felis domestica) because it can decrease the sperm quality significantly.

Keywords


Contraception; extract of kapok seed; domestic cat; sperm; motility; viability; abnormality

Full Text:

PDF

References


Afiati F., Herdis dan Said S. 2013. Pembibitan Ternak dengan Inseminasi Buatan. Jakarta (ID): Penebar Swadaya.

Anief, M. 2000. Ilmu Meracik Obat. Yogyakarta (ID): UGM Pr. (Hal 135-136)

Anonim. 2000. Petunjuk Praktikum Fisiologi I. Yogyakarta (ID): Universitas Gadjah Mada. (Hal 22 – 24)

Anonim. 2000. Petunjuk Praktikum Fisiologi II. Yogyakarta (ID): Universitas Gadjah Mada. (Hal 12 – 18)

Chang, MC., Zhiping Gu, SK. Saksena. 1980. Effects of Gossypol on the Fertility of Male Rats, Hamsters and Rabbit. (Diakses 27 April 2014, http://www.contraceptionjournal.org/article/0010-7824(80)90011-6/abstract).

Choubey, A. 2011. In vitro growth and inhibition studies of Ceiba pentandra on Monosodium Urate Monohydrate Crystals. Pharmacology online 2.

Coutinho, E., Segal dan Melo, JF. 1981. Biphasic action of gossypol in men. Fertil. Steril.

Fahim, MS., Wang, M., Sutcu, MF., Fahim, Z., danYoungquist, RS. 1993. Sterilization of dogs with intra-epididymal injection of zinc arginine. Epub Contraception. 47(1):107-22.

Frick, J., Danner Ch., Kohle R., dan Kunit C. 1981. Male fertility regulation. In CotesPrieto, J., Campos da Paz, A. and Neves-e-Castro, M. (eds.) Research on Fertility and Sterility, p. 291, ( Lancaster: MTP )

Grady AV., dan Nelson L. 1972. Cationic influences on sperm biopotensial. Exp Cell Res 73 : 192-195.

Guyton, AC., dan Hall, J. 2000. Textbook of Medical Physiology. 10th ed. W.B. Saunders Company. Philadelphia

Irwan, SA. 2010. Tanaman Kapas dan Kaitannya Dengan Gosipol. Bogor (ID). Balai Penelitian Ternak.

Jeyendran, RS., dan Zaneveld, LJD. 1986. Instruction for Hypoosmatic Swelling (HOS) Test. Short Course: Reproduction/Andrology and non hormonal contraception. Chicago.

Liu, ZQ., Liu, GZ., Hei, LS., Zang, RA., dan Yu, CZ. 1981. Clinical trial of gossypol as a male antifertility agent. In Chang, EF., Griffin, D., dan Woolman A. eds. Recent Advances in Fertility Regulation, Proc. Symp. Beijing. Geneva, Atar.

Malini, DM. 2000. Pengaruh Ekstrak Biji Nimba (Azadirachta indica A. Juss) terhadap Laju Pertambahan Berat Badan dan Organ Reproduksi Tikus (Rattus norvegicus) Wistar Jantan. J. Biol. Unud IV(2):78-83

Merck Index. 1985. An Encyciopedia of Chemical and Drugs.9th Ed. New Yersey.

National Coordinating Group on Male Antifertility Agents . 1978. Gossypol—A new antifertility agent for males. Chin. Med. J. (Engl. Edn.), 6, 417–28

Philips, JT., Leeds EB. 1976. A closed technique for canine orchidectomy. Canine Pract 3:23

Prabudiasto. 2012. Extracta: ekstrak. (Diakses 24 Februari 2014. prabudiasto.wordpress.com/2012/06/29/ekstrak/)

Robertis, ED., dan Robertis, EM. 1979. Cell and moleculer Biology. Philadelphia: Sauders Colleg: 151-159.

Saili, T., Hamzah dan Aku, AS. 2008. Kualitas Spermatozoa Epididimis Sapi Peranakan Ongole yang Disimpan pda Suhu 3-5oC. Prosiding Seminar Nasional Sapi Potong.Hal. 78-85. Fakultas Pertanian Universitas Haluleo. Kendari.

Tepsumethanon, V., Wilde, H., dan Hemachudha, T. 2005. Intratesticular injection of a balanced zinc solution for permanent sterilization of dogs. J Med Assoc Thai. 88(5):686-9.

Velasquez-Pereira, J., Chenoweth, PJ., McDowell, LR., Risso, CA., Staples, CA., Prichard, D., Martin, FG., Calhoun, MC., Williams, SN., Wilkinson, NS. 1998. Reproductive effect of feeding gossypol and vitamin E to bulls. J. Anim. Sci., 76 (11): 2894-2904.


Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

science and company

JRVI Stats

 

________________________________________________________________

Jurnal Riset Veteriner Indonesia
Veterinary Medicine, Faculty of Medicine. Hasanuddin University
ISSN Print: 2614-0187, ISSN Online: 2615-2835

Jl. Perintis Kemerdekaan KM.10 Tamalanrea, Makassar 90245