Potency of 1 - deoxynojirimycin Compounds for Slowed Hydrolysis of Various Carbohydrates by Raw Enzymes of the Rumen Liquid

Authors

S. Syahrir
F. K. Tangdilintin
K. G. Wiryawan
A. Parakkasi
M. Winugroho

DOI:

https://doi.org/10.20956/jitp.v1i2.666

Abstract

Compound of 1- deoxynojirimycin (DNJ) in the mulberry leave extract (MLE) potentially hamper the hydrolysis process of various carbohydrates. This experiment aimed to study dynamic concentration of reductive sugar resulted from the hydrolysis of various carbohydrates by raw enzymes of the rumen liquid, with or without the addition of mulberry leave extract containing 1-deoxynojirimycin. Materials tested are maltose, sucrose, starch and cellulose. The result of the experiment showed that the addition of mulberry leave extract on the media with the substrate of maltose hampered the activity of maltase. It was concluded that MLE containing DNJ can be used as an agent of slow release mechanism of non-structural carbohydrates, especially maltose, in the rumen system.

References

Anonim. 2006. Rencana Tindak Lanjut Program Menuju Kecukupan Daging Sapi 2010. Laporan Bulanan Puslitbangnak.

AOAC. 1999. Official Methods of Analysis. Association of Official Analytical Chemist. Arlington, Virginia.

Arai, M., M. Shinya, T. Genzou, U. Yoshihiro, K. Tatsuya, T. Hisato, F. Takako, H. Masaya, Y. Yoshiaki, and Fujiwara. 1998. N-Methyl-1-deoxynojirimycin (MOR-14), an α-Glucosidase Inhibitor, Markedly Reduced Infarct Size in Rabbit Hearts. American Heart Association, Inc., 97: 1290-1297.

Breitmeier, D. 1997. Acarbose and 1-deoxynojirimycin inhibit maltose and maltooligosaccharide hydrolysis of human intestinal glucoamylase-maltase in two different substrate-induced modes. Archives Biochem. & Biophys., 346(1): 7-14.

Chapel,, C., G. Céline, R. Philippe, Z. Nicole, D. Jean, A.D. Raymond, T. Christian, Z. Fabien, and D. David. 2006. Antiviral effect of -glucosidase inhibitors on viral morphogenesis and binding properties of hepatitis C virus-like particles. J. Gen. Virol., 87: 861-871.

Close, W.H. and K.H. Menke. 1986. Manual Selected Topics in Animal Nutrition. University of Hohenheim. The Institute of Animal Nutrition, Stuftgart, Germany.

Gross, V., T. Andus, T.A. Tran-Thi, R.T. Schwarz, K. Decker, and P.C. Henrich. 1983. 1-deoxynojirimycin impairs oligosaccharide processing of alpha 1-proteinase inhibitor and inhibits its secretion in primary cultures of rat hepatocytes. J. Biol. Chem., Vol. 258 Issue 20: 12203-12209.

Horne, P. M., K. R. Pond, and L. P. Batubara. 1995. Sheep Under Rubber: Prospects and Research Proirities in Indonesia. In: Integration of Ruminants into Plantation Systems in Southeast Asia, Eds. B.F. Mullen and H. H. Shelton. pp. 58-64.

Hvelplund, T. and J. Madsen. 1985. Amino acid passage to the small intestine in dairy cows compared with estimates of microbial protein and undegraded dietary protein from analysis on the feed. Acta Agric. Scand. Suppl., 25: 21-36.

Jordan, J.E., S.A. Simandle., C.D. Tulbert, D.W. Busija, and A.W. Miller. 2003. Fructose-fed rats are protected againts ischemia/reperfusion injury. J. of Pharmac. and Exp. Therapeutics, Vol. 307: 1007-1011.

Jean-Blain, C. 1991. Rumen Disfunctions. In: Rumen Microbial Metabolism and Ruminant Digestion, Ed. J.P. Jouany. INRA Editions, Paris. pp. 361-364.

Kimura, T., N. Kiyotaka, S. Yuko, Y. Kenji, S. Masahiro, Y. Kohji, S. Hiroshi, and M. Teruo. 2004. Determination of 1- deoxynojirimycin in mulberry leaves using hydrophilic interaction chromatography with evaporative light scattering detection. J. of Agric. Food Chem., 52: 1415-1418.

Oku, T., Y. Mai, N. Mariko, S. Naoki, and N. Sadako. 2006. Inhibitory effects of extractives from leaves of Morus alba on human and rat small intestinal disaccaridase activity. J. of Nutr., 95: 933-938.

Romaniouk, A.V., A. Silva, J. Feng, and I. K. Vijay. 2004. Synthesis of a novel photoaffinity derivative of 1-deoxynojirimycin for active site-directed labeling of glucosidase I. Glycobiology, Vol. 14 No. 4: 301-310.

Stewart, C. S. 1991. The Rumen Bacteria. In: Rumen Microbial Metabolism and Ruminant Digestion, Ed. J.P. Jouany. INRA Editions, Paris. pp. 15-26.

Suyadi, Soedomo dan A. Mahmud. 1989. Produksi Biji Legum Desmanthus virgatus. In: Forage Production, Eds. M. Wodzicka, Tomaszewska, and J. A. Thompson. Proceeding of a Workshop Conducted at IPB, Bogor, Indonesia. IPB-Australian Project.

Tilley, J.M.A. and R.A. Terry. 1963. Two Stage Technique for In vitro Digestion of Forage Crops. In: Manual Selected Topics in Animal Nutrition, Eds. W.H. Close and K.H. Menke. University oh Hohenheim, The Institute of Animal Nutrition, Stuftgart, Germany.

Wiryawan, K.G., and J.D. Brooker. 1996. Probiotic control of lactate accumulation in acutely grain fed sheep. Aust. J. Agric. Res., 46: 1555-1568.