Growth of Candida sp and Aspergillus sp from Bronchoscopy Pulmonary Tuberculosis Patients on Bran Media

Abstract

Pulmonary tuberculosis caused by Mycobacterium tuberculosis become a
health problem in Indonesia. The chronic nature of this disease is further exacerbated if
it is accompanied by fungal infection such as Candida albicans and Aspergillus sp.,
which is usually remains undiagnosed and thus untreated. Culture techniques can be
used to identify Candida sp and Aspergillus sp from bronchoscopy. Fungal culture
media in laboratory containing high carbohydrate source, nitrogen source are required
for the growth. This nutrient can be found in bran that contains high carbohydrates,
proteins, fats, vitamins, and crude fiber. So that bran can be used as raw material for 

alternative fungal growth media. The purpose of this study was to increase bran as a
medium for the growth of Candida sp and Aspergillus sp isolated from bronchoscopy of
pulmonary TB patients. This study included bran collection, preparation of bran media,
inoculation bronchoscopy on bran media, observation of fungal growth. Colonies of
Candida sp and Aspergillus sp were confirmed microscopically. The results showed that
Candida sp and Aspergillus sp grew on both media, Bekatul Dextrose Agar and Potato
Dextrose Agar. The conclusion of this study is that bran can be used as a medium for
fungal growth. Bran media can be used as an alternative media to replace synthetic
media to grow Candida sp and Aspergillus sp isolated from bronchial rinses.