ANALISIS KERAGAMAN GENETIK PADA IKAN KERAPU MACAN (Epinephelus fuscoguttatus) YANG TAHAN TERHADAP PENYAKIT YANG DISEBABKAN BAKTERI Vibrio alginolyticus

Abstract

The research was purposed to analyse genetic similarity of tiger grouper (Epinephelus fuscoguttatus) in
order to select grouper that was resistant to Vibrio alginolyticus. In this study, fish were challenged with Vibrio
alginolyticus and genetic similarity was examined by a PCR-RAPD method. Eight RAPD primers were used for
PCR-RAPD analysis. The results showed that only three primers of RAPD primer (YNZ-22, UBC-456, dan
UBC-457) generated high number of RAPD fragments. The resistant group of tiger groupers generated higher
polymorph RAPD fragments than the susceptible groups. RAPD primer YNZ-22 and UBC-457 generated 82 %
and 71 % of RAPD polymorph fragments from resistant group of fish and 71% and 60 % from susceptible
groups, respectively. Primer YNZ-22 is a best genetic marker to analyse genetic similarity of tiger groupers
produced specific marker ranging from 1,2-2,0 kb. Genetic distances between the population of resistant fish
and population of susceptible fish was 0.5091 and between individual of the resistant fish and susceptible fish
was 0.7032.
Keywords: Genetik diversity, Epinephelus fuscoguttatus, V. alginolyticus, PCR, RAPD.