The Effect of Sample Preparation, Extraction Duration, and Precipitant Toward Carrageenan Characteristics which Extracted from Kappaphycus alvarezii

Authors

  • Abraham Horisanto Program Studi Teknologi Pangan, Politeknik Negeri Sriwijaya
  • Metusalach Metusalach Departemen Perikanan, Fakultas Ilmu Kelautan dan Perikanan, Universitas Hasanuddin
  • Elmi Nurhaidah Zainuddin Departemen Perikanan, Fakultas Ilmu Kelautan dan Perikanan, Universitas Hasanuddin,
  • Willy Wijayanti Program Studi Teknologi Pangan, Politeknik Negeri Sriwijaya
  • Ilham Wahyu Pamungkas Program Studi Teknologi Pangan, Politeknik Negeri Sriwijaya
  • Venny Agustin Program Studi Teknologi Pangan, Politeknik Negeri Sriwijaya
  • Syerina Raihatul Jannah Program Studi Teknologi Pangan, Politeknik Negeri Sriwijaya

DOI:

https://doi.org/10.35911/torani.v8i2.43915

Keywords:

Carrageenan, sample preparation, extraction duration, precipitant

Abstract

Carrageenan is a polysaccharide compound product produced from carrageenophyte seaweed extraction, one of which is Kappaphycus alvarezii. The yield and other characteristics are conventionally produced from an extraction process and have different results. This study aims to explore and optimize the characteristics of carrageenan produced by treating sample preparation with two types of precipitants at specific concentrations, setting the timing during the extraction process, and examining the interaction of these three treatments on the carrageenan characteristics. The results obtained showed that the yield value, acid-insoluble ash (AIA) content, and viscosity were influenced by the preparation method, specifically by chopping the seaweed before extraction. Additionally, the characteristics of the gel strength were influenced by the preparation method, specifically by blending the seaweed. The use of KCl precipitant affects the water content, AIA content, and viscosity, while the CaCl₂ precipitant affects the yield value and gel strength. 1-hour extraction process affected the characteristics of carrageenan for AIA content and gel strength, while extraction for 2 hours affected the yield value, water content, and viscosity. The interaction of 8% KOH solvent by cutting sample treatment, extracted for 2 hours and using KCl precipitant, produced good carrageenan characteristics for water content, AIA content, and viscosity.

References

A.O.A.C. 1990. Official Methods of Analysis. 15th Edition, Association of Official Analytical Chemist, Washington DC.

Asikin AN, Kusumaningrum I, Sutono, D. 2015. Ekstraksi dan Karakterisasi Sifat Fungsional Karaginan Kappaphycus alvarezii Asal Pesisir Kabupaten Kutai Timur. Jurnal Ilmu dan Teknologi Kelautan Tropis, 7(1):49-58.

Astuti KW, Wijayanti NPAD, Putra IGNAD, Laksmiani NPL. 2017. Optimization of Isolation Method of Carrageenan from Kappaphycus alvarezii Doty Using Factorial Experimental Design. Journal of Health Sciences and Medicine 1(2):4-7.

Awalludin A, Mingu N, Yaser AZ, Mamat H, Kamaruzaman KA, Jamain Z, Rahman MdL, Majid MHA, Sarjadi MS. 2021. Extraction and Physicochemical Properties of Refined Kappa-Carrageenan from Kappaphycus alvarezii Originated from Semporna, Sabah. Journal of Applied Science and Engineering, 25(3):511-516.

Cunha L, Grenha A. 2016. Sulfate Seaweed Polysaccharides as Multifunctional Materials in Drug Delivery Applications. Marine Drugs 14(3):42p. https://doi.org/10.3390/ md14030042

Devi FP, Riyadi DN, Kurniawansyah F, Roesyadi A. 2020. Produksi Kappa Karaginan dari Rumput Laut (Eucheuma cottonii) dengan Metode Semi-Refined Carrageenan. Journals of Fundamentals and Applications of Chemical Engineering, 1(1):1-4.

Djurumundi SL, Montolalu RI, Pongoh J, Dotulong V, Lohoo HJ, Makapedua DM. 2022. Mutu Karagenan Dengan Menggunakan Pelarut dan Metode Ekstraksi Berbeda. Media Teknologi Hasil Perikanan, 10(2): 80-85. https://ejournal.unsrat.ac.id/index. php/jmthp/index

Fathoni, DA, Arisandi A. 2020. Kualitas Karaginan Rumput Laut (Eucheuma cottonii) Pada Lahan yang Berbeda di Kecamatan Bluto Kabupaten Sumenep. Juvenil, 1(4):548-557. https://journal.trunojoyo.ac.id/juvenil.

Febrianti AA, Nofreeana A, Armando E. 2023. Pengaruh Karagenan dari Rumput Laut Kappaphycus alvarezii terhadap kesegaran Ikan Gurami (Osphronemus gouramy). JPB Kelautan dan Perikanan, 18(2):161-167.

Food and Agriculture Organization (FAO). 2007. Carrageenan. Prepared at the 79th JECFA and published in FAO JECFA Monographs.

Food Marine Colloids Corp (FMC Corp). 1977. Carrageenan. Marine Colloid Monograph Number One. Springfield New Jersey. USA : Marine Colloid Division FMC Corporation page. 23-29. New Jersey. USA.

Hiariey SL, Lekahena VNJ, Safar I. 2021. Characteristic of physicochemical ATC Kappaphycus alvarezii from Kolorai Village South Morotai District on different harvesting time. IOP Conf. Series: Earth and Environmental Science 797 (2021) 012021. doi:10.1088/1755-1315/797/1/012021.

Kasim S, Subehan, Alam G, Rifai Y, Ismail, Sarce, Utami RN. 2023. Optimization of carrageenan extraction from Kappaphycus alvarezii red algae using microwave assisted extraction method with variation of solvent concentration. Scirea 8(2)115-128. http://www.scirea.org/journal/Physics

Kaya AOW. 2018. Pemanfaatan Karaginan Semi Murni Sebagai Bahan Pembentuk Gel Dalam Pembuatan Gel Pengharum Ruangan. Majalah BIAM 14(01)37-44.

Kumayanjati B, Dwimayasanti R. 2018. Kualitas Karaginan dari Rumput Laut Kappaphycus alvarezii Pada Lokasi Berbeda di Perairan Maluku Tenggara. JPB Kelautan dan Perikanan, 13(1):21-32.

Meiyasa, F. dan Tarigan, N. 2018. Peranan Kalium Hidroksida (KOH) Terhadap Mutu Karaginan Eucheuma cottonii Di Indonesia. Agrisaintifika, Jurnal Ilmu-Ilmu Pertanian 2(2):131-136.

Syaharuddin. 2019. Optimization of extraction and quality assessment based on physicochemical properties of Carrageenan from red algae (Kappaphycus alvarezii) origin of South Sulawesi Indonesia. The 3rd International Conference on Science, Journal of Physics: Conference Series. doi:10.1088/1742-6596/1341/7/072013.

Zakaria A, Rahman EN, Rahmani UN, Manurung R, Puad NIM, Abduh MY. 2019. Production of Carrageenan by Different Strains of Kappaphycus alvarezii Cultivated in Serang, Indonesia. IIUM Engineering Journal, 20(2):12-21.

Downloads

Published

2025-06-29

How to Cite

Horisanto, A., Metusalach, M., Zainuddin, E. N., Wijayanti, W., Pamungkas, I. W., Agustin, V., & Jannah, S. R. (2025). The Effect of Sample Preparation, Extraction Duration, and Precipitant Toward Carrageenan Characteristics which Extracted from Kappaphycus alvarezii. Torani Journal of Fisheries and Marine Science, 8(2), 1–15. https://doi.org/10.35911/torani.v8i2.43915