Andi Nur Asrinawaty, Muh Nasrum Massi, Rizalinda Sjahril


Introduction: NTM is a group of mycobacterium other than M.tuberculosis complex and M.Leprae, which found in the environment and can be pathogenic. Objectives: This study aim was to detect NTM isolates using PCR on target genes IS 6110. Methods: This study was conducted in Laboratory of Microbiology, Faculty of Medicine, Hasanuddin University and Laboratorium microbiology in Hasanuddin University Hospital. 14 sample isolates of NTM from sputum of suspected tuberculosis patients. The method using PCR IS6110 gene. Results: The results showed that of 14 samples, 8 positive samples with M. tuberculosis and 6 samples negative. Conclusions: IS6110 PCR can be used to detect NTM isolates from sputum of suspected tuberculosis patients.

Keywords : NTM, PCR, IS6110, M.tuberculosis

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Jagielski Tomasz, Jakko van Ingen,Nalin Rastogi, et al. Current Methods in the Molecular Typing of Mycobacterium tuberculosis and Other Mycobacteria.Biomed Res Int. 2014; 645-802.

Bensi E.P. A, Patricia C. P., dan Marcelo de Carvalho Ramos. Incidence of Tuberculous And Non- Tuberculous Mycobacteria, Differentiated By Multiplex Pcr, In Clinical Specimens Of A Large General Hospital.Clinics (Sao Paulo).2013 Feb; 68(2): 179–183.

Bahram Nasr Esfani,et al. Isolation And Phenotypic Identification Of on-Tuberculous Mycobacteria Existing In IsfanDifferentWater

Samples.Advanced Biomedical Research| January - March 2012 | Vol 1 | Issue 1.

Koh WJ, O Jung Kwon, Kyung Soo Lee. Diagnosis and Treatment of

Nontuberculous Mycobacterial Pulmonary Diseases: A Korean Perspective. J Korean Med Sci 2005; 20: 913-25. ISSN 1011-8934.

Da-costa ARF, Maria Luiza Lopes, Maísa Silva de Sousa, et al.

Pulmonary Nontuberculous Mycobacterial Infections in the State of Endemic Region for Tuberculosis in North of /pdf/32001.diakses Maret 2014.

Aliyu Gambo, Samer S. El-Kamary, Alash’le Abimiku,et al. Prevalence of Non-Tuberculous Mycobacterial Infections among Tuberculosis Suspects in Nigeria, May 2013, Volume 8, Issue 5, e63170.

WHO. GlobalTuberculosis Report 2014. 10665/137094/1/9789241564809_en g.pdf.

Putra E, et al. Perbandingan Resistensi Mycobacterium Atipik Dengan ycobacterium Non Atipik Terhadap Obat Anti Tuberkulosis. Majalah edokteran Andalas 2004; No.1 Vol.28

Misnadiarly AS dan Suwando. MycobacteriumchelonaeDi

Beberapa Pusat Pelayanan Kesehatan Di Indonesia, Salah Satu Ancaman Infeksi Pada Luka Bedah. Dexa Media, No.1 Vol.19 Januari-Maret 2006. ISSN 0215-7551.

Kumar Vijay, TejashreeAUrs, Rajani R Ranganath. MPT 64 Antigen detection for Rapid confirmation of M. tuberculosis isolates. BMC research note 2011 4:79.

CorosA., DeConno E., Derbyshire KM. IS6110, a Mycobacterium tuberculosis Complex-Specific Insertion Sequence, Is Also Present in the Genome of Mycobacterium smegmatis, Suggestive of Lateral Gene Transfer among Mycobacterial Species, Journal Of Bacteriology, May 2008, p. 3408–3410, Vol.190, No.9.

Yeboah-Manu D, Yates MD, Wilson SM. Application of a Simple Multiplex PCR to Aid in Routine Work of the Mycobacterium Reference Laboratory. J Clin Microbiol. 2001;39(11):4166-8.

Park Young Mi. Evaluation of an Immunochromatographic Assay Kit for Rapid Identification of Mycobacterium tuberculosis Complex in Clinical Isolates. Journal of Clinical Microbiology. Vol 47 No.2. Feb 2009. p. 481-484.

Changtai Zhu, Jinming Liu, Yang Ling et al. Evaluation Of The Clinical Value Of Elisa Based On Mpt64 Antibody Aptamer For Serological Diagnosis Of Pulmonary Tuberculosis.BMC Infect Dis. 2013 September 12; 13: 430.



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Nusantara Medical Science Journal
Faculty of Medicine. Hasanuddin University
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