Rusdianto Rusdianto, Muh Nasrum Massi, Ahyar Ahmad




Introduction: Diarrhea is one health problem in the world with mortality and morbidity rates are high, both in developed and developing countries. This research aims to detect Shigella sp in the stool of children with diarrhea using culture method and PCR. Methods: This research is a descriptive with cross-sectional design. A total of 50 samples were obtained from four health centers in Makassar city, i.e Barabaraya, Pampang, Antang Perumnas and Tamangapa from May - July 2016 with rectal swab method. For culture test, the samples were inoculated on medium SSA. While with PCR test, primer icsA (526 bp) was used. Results: The results indicate that, from 50 samples tested by using culture method, there is no sample (0%) posi- tive detected Shigella sp. While the PCR method there 6 samples (24%) positive detected Shigella sp. Conclusion: The sensitivity of PCR in the detection of Shigella sp from the level of dilution 100 - 10-3. Al together, it can be inferred that PCR method is more accurate method detect Shigellasp than other culture methode.


Keywords: Diarrhea, Shigellasp, Culture, PCR

Full Text:



Bryce J, Boschi-Pinto C, Shibuya K, Black RE, Group WCHER. WHO estimates of the causes of death in children. The Lancet.


Tjaniadi P, Lesmana M, Subekti D, Machpud N, Komalarini S, Santoso W, et al. Antimicrobial resistance of bacterial pathogens associated with diarrheal pa- tients in Indonesia. The American journal of tropical medicine and hygiene.


Levine MM, Kotloff KL, Barry EM, Pasetti MF, Sztein MB. Clinical trials of Shigella vac- cines: two steps forward and one step back on a long, hard road. Nature Reviews Mi- crobiology. 2007;5(7):540-553.

Ojha SC, Yean Yean C, Ismail A, Banga Singh K-K. A pentaplex PCR assay for the detection and differentiation of Shigella species. BioMed research international.

; 2013.

Thiem VD, Sethabutr O, von Seidlein L, Van Tung T, Chien BT, Lee H, et al. Detection of Shigella by a PCR assay targeting the ipaH gene suggests increased prevalence of shigellosis in Nha Trang, Vietnam. Jour- nal of clinical microbiology. 2004;42(5):


Sudaryat S. Gastroenterologi Anak. Lab/ SMF Ilmu Kesehatan Anak Fakultas Kedokteran Universitas Udayana. 2010.

Nascimento AM, Van Der Sand ST. De- tection of the virulence plasmid pINV, us- ing inv E gene in shigella sp. in sewage samples by PCR assay. Biociências (On- line). 2008;16(1).

Wyllie R, Hyams JS, Kay M. Pediatric gas- trointestinal and liver disease: Elsevier Health Sciences; 2015.

Kemenkes R. Buletin jendeta data dan informasi kesehatan: situasi diare di Indo- nesia. Jakarta: Pusat Data dan Informasi Kementrian Kesehatan RI Hlm. 2011:1-6.

Palupi A, Hadi H, Soenarto SS. Status gizi dan hubungannya dengan kejadian diare pada anak diare akut di ruang rawat inap RSUP Dr. Sardjito Yogyakarta. Jurnal Gizi Klinik Indonesia. 2009;6(1):1-7.

Alipour M, Talebjannat M, Nabiuni M. Poly- merase chain reaction method for the rapid detection of virulent Shigella spp. 2012.

Gaurav A, Singh S, Gill J, Kumar R, Kumar D. Isolation and identification of Shigella spp. from human fecal samples collected from Pantnagar, India. Vet World. 2013;6:376-379.

Bhutta ZA. Acute gastroenteritis in children. Nelson textbook of pediatrics. 2007;19:1323-1339.

Lindsay B, Ochieng JB, Ikumapayi UN, Toure A, Ahmed D, Li S, et al. Quantitative PCR for detection of Shigella improves as-

certainment of Shigella burden in children with moderate-to-severe diarrhea in low- income countries. Journal of clinical mi- crobiology. 2013;51(6):1740-1746.

Dutta S, Chatterjee A, Dutta P, Rajendran K, Roy S, Pramanik K, et al. Sensitivity and performance characteristics of a di-rect PCR with stool samples in compari- son to conventional techniques for diag- nosis of Shigella and enteroinvasive Es- cherichia coli infection in children with acute diarrhoea in Calcutta, India. Journal of medical microbiology. 2001;50(8):667-674.



  • There are currently no refbacks.

View My Stats

Flag Counter


Nusantara Medical Science Journal
Faculty of Medicine. Hasanuddin University
ISSN (Print) 2460-9757 | ISSN (Online) 2597-7288

Jl. Perintis Kemerdekaan KM.10 Tamalanrea, Makassar 90245
Telp/fax. (+62 411) 586010