DETEKSI FENOTIP ISOLAT Pseudomonas aeruginosa PENGHASIL METALLO BETA-LAKTAMASE (MBL) RESISTEN KARBAPENEM PADA PASIEN INFEKSI DI RSUP Dr. WAHIDIN SUDIROHUSODO MAKASSAR
Keywords:
Antibiotik, Karbapenem, Pseudomonas aeruginosa, MBL, CDTAbstract
Resistensi antibiotik pada beberapa tahun terakhir menjadi ancaman yang muncul dan menyebabkan kekhawatiran bagi dunia kesehatan karena semakin meningkatnya bakteri yang resisten terhadap hampir semua golongan antibiotik. Penelitian ini bertujuan untuk mengetahui prevalensi kejadian resistensi Pseudomonas aeruginosa terhadap antibiotik golongan karbapenem dan prevalensi fenotip isolat P. aeruginosa yang memproduksi Metallo Beta-Laktamase (MBL) pada pasien infeksi di RSUP Dr. Wahidin Sudirohusodo. Penelitian dilaksanakan di laboratorium patologi klinik RSUP Dr. Wahidin Sudirohusodo Makassar periode Mei-Juli 2019. Penelitian ini menggunakan desain eksperimental laboratorium dengan teknik pengambilan sampel secara consecutive sampling. Bakteri P. aeruginosa diisolasi dari 50 pasien. Pengujian dilakukan meliputi uji sensitivitas antibiotik dengan menggunakan vitek 2 compact dan uji fenotip deteksi MBL dengan menggunakan metode Combined Disk test (CDT). Hasil penelitian bakteri P. aeruginosa yang menginfeksi pasien di RSUP Dr. Wahidin Sudirohusodo Makassar telah mengalami resistensi terhadap antibiotika golongan karbapenem, sebesar 26% (13/50) dan 46,15% (6/13) Isolat P. aeruginosa yang resistensi terhadap antibiotika golongan karbapenem positif menghasilkan MBL.
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