STUDI PRAKLINIK EFEK SIMVASTATIN, ROSUVASTATIN, DAN FENOFIBRAT TERHADAP KADAR LAKTAT DEHIDROGENASE PADA TIKUS BETINA YANG DIINDUKSI KONTRASEPSI ORAL DAN DIET TINGGI LEMAK
Keywords:
Kontrasepsi oral, diet tinggi lemak, laktat dehidrogenase, statin, fibratAbstract
Penggunaan pil kontrasepsi dapat menyebabkan peningkatan inflamasi yang terkait dengan resiko penyakit degeneratif pada wanita. Laktat dehidrogenase (LDH) merupakan biomarker peningkatan inflamasi dan kerusakan jaringan. Penelitian ini bertujuan mengetahui pengaruh pil kontrasepsi oral serta mengevaluasi efektivitas dari simvastatin, rosuvastatin, dan fenofibrat terhadap penurunan kadar LDH pada tikus betina (Rattus novergicus). Tikus (n=20) dibagi menjadi empat kelompok yaitu satu kelompok kontrol (pil kontrasepsi, pakan diet tinggi lemak dan NaCMC 0,5%), dan tiga kelompok perlakuan yang diberi pil kontrasepsi, pakan diet tinggi lemak dan obat uji: simvastatin (0,21 mg/kgBB), rosuvastatin (0,514 mg/kgBB) atau fenofibrat (8,246 mg/kgBB). Pemberian pil kontrasepsi dan pakan diet tinggi lemak dilakukan 30 hari.Pada hari ke-31, tikus diberi pengobatan sesuai kelompok perlakuan: kelompok kontrol diberikan plasebo (NaCMC), dan ketiga kelompok perlakuan, masing-masing diberikan obat uji simvastatin (0,21 mg/kgBB), rosuvastatin (0,51 mg/kgBB) serta fenofibrat (8,246 mg/kgBB) selama hari ke-30 hingga hari ke-60. Hasil menunjukkan bahwa pemberian pil kontrasepsi dan pakan diet tinggi lemak selama 30 hari menyebabkan peningkatan LDH pada semua kelompok tikus dan mencapai nilai signifikan pada kelompok simvastatin, rosuvastatin, dan fenofibrat (P<0,05). Setelah pemberian pengobatan selama 30 hari, kelompok simvastatin, rosuvastatin, maupun fenofibrat mengalami penurunan kadar LDH berturut-turut sebesar 25,8%, 27,1%, dan 27,9%. Penurunan kadar LDH dari ketiga jenis terapi tidak berbeda signifikan secara statistik. Dapat disimpulkan bahwa baik simvastatin, rosuvastatin maupun fenofibrat memiliki potensi menurunkan kadar LDH yang setara pada hewan model inflamasi sistemik yang diinduksi kontrasepsi oral dan diet tinggi lemak.
References
Kartika Y, Ronoatmodjo S. Penggunaan Kontrasepsi Hormonal dan Obesitas pada Wanita Usia Subur di Indonesia. 2019. Jurnal Kesehatan Reproduksi. 2019;10(2):101-110. DOI: 10.22435/kespro.v10i2.2412.
Gupta S. Obesity and Female Hormones. The Obstetrician and Gynaecologist. 2006;8(1):26-31.DOI:10.1576/toag.8.1.026.27205.
Santos ACN dos, Petto J, Oliveira de Oliveira FT, Diogo DP, Ladeia AMT. C-Reactive Protein in Oral Contraceptive Users: Related Factors and Cardiovascular Risk. International Journal of Cardiovascular Sciences. 2016;29(4):320-325. DOI: 10.5935/2359-4802.20160051.
Wu LW, Kao TW, Lin CM, Yang HF, Sun YS, Liaw FY, et al. Examining the Association between Serum Lactic Dehydrogenase and All-Cause Mortality in Patients with Metabolic Syndrome: A Retrospective Observational Study. BMJ Open. 2016;6(5)1-7. DOI: 10.1136/bmjopen-2016-011186.
Humaish HH, Alasadi Amer, Aldafae Intithar. Evaluation the Relationship between Oral Contraceptives Containing Drospirenone with Dyslipidemia and Risk of Cardiovascular Diseases Among Women in Al-Kut City. Indian Journal of Forensic Medicine and Toxicology. 2020;14(4): 1920-1926. DOI: 10.37506/ijfmt.v14i4.11827.
Shrivastava AK, Singh HV, Raizada A, Singh SK. C-Reactive Protein, Inflammation and Coronary Heart Disease. The Egyptian Heart Journal. 2015;67(2):89-97. DOI: 10.1016/j.ehj.2014.11.005.
Chinetti-Gbaguidi G, Fruchart JC, Staels B. Pleiotropic Effects of Fibrates. Current Atherosclerosis Reports.2005;7(5):396-401. DOI: 10.1007/s11883-005-0053-x.
Lim Sang Yup. Role of Statins in Coronary Artery Disease. Chonnam Medical journal. 2013;49(1): 1-6. DOI: 10.4068/cmj.2013.49.1.1.
Sahebkar A, Giua R, Pedone C. Impact of Statin Therapy on Plasma Leptin Concentrations: a Systematic Review and Meta-Analysis of Randomized Placebo-Controlled Trials. British Journal of Clinical Pharmacology. 2016;82(6):1674-1684. DOI: 10.1111/bcp.13086
Wang L, Lin R, Guo L, Hong M. Rosuvastatin Relieves Myocardial Ischemia/Reperfusion Injury by Upregulating PPAR-Gamma and UCP2. Molecular Medicine Report. 2018;18(1):789-798. DOI: 10.3892/mmr.2018.9062.
Škop V, Trnovská J, Oliyarnyk O, Marková I, Malínská H, Kazdová L, et al. Hepatotoxic Effects of Fenofibrate in Spontaneously Hypertensive Rats Expressing Human C-Reactive Protein. Physiological Research. 2016;65(6):891-899. DOI: 10.33549/physiolres.933304.
Duan Y, Zeng L, Zheng C, Song B, Li F, Kong X. Inflammatory Links Between High Fat Diets and Diseases. Frontiers in Immunology. 2018;13(9):1-10. DOI: 10.3389/fimmu.2018.02649.
Khalil A, Omran H, Alsheikh F. Balance of Pro- and Anti-Inflammatory Cytokines in Livers of High Fat Diet Rats Exposed to Fractionated Gamma Irradiation. BMC Research Notes. 2018;11(1):1-6. DOI: 10.1186/s13104-018-3851-2.
Nair A, Jacob S. A Simple Practice Guide for Dose Conversion Between Animals and Human. Journal of Basic and Clinical Pharmacy. 2016;7(2):27-31. DOI: 10.4103/0976-0105.177703.
Chahyanto BA, Marliyati SA, Winarsih W et al. Efek Diet Tinggi Kolesterol Terhadap Peningkatan Kolesterol Darah, Gambaran Histopatologi Hati, dan Bobot Badan Kelinci New Zealand White Jantan. Jurnal Sain Veteriner. 2017;34(1):50-59. DOI: 10.22146/jsv.22814.
Heryani R. Pengaruh Ekstrak Buah Naga Merah Terhadap Profil Lipid Darah Tikus Putih Hiperlipidemia. Jurnal Iptek Terapan. 2016;10(1):8–17. DOI: 10.22216/jit.2016.v10i1.372.
Divani AA, Luo X, Datta YH, Flaherty JD, Panoskaltsis-mortari A. Effect of Oral and Vaginal Hormonal Contraceptives on Inflammatory Blood Biomarkers. Hindawi Publishing Corporation Mediators of Inflammation. 2015;2015:1-8.DOI: 10.1155/2015/379501.
Naz F, Jyoti S, Rahul, Akhtar N, Siddique YH. Effect of Oral Contraceptive Pills on the Blood Serum Enzymes and DNA Damage in Lymphocytes Among Users. Indian Journal of Clinical Biochemistry. 2016;31(3):294–301. DOI: 10.1007/s12291-015-0533-x.
Harahap Novita Sari, Marpaung Deni Rahman. Respon Laktat Dehidrogenase (LDH) setelah Aktifitas Fisik Intensitas Berat pada Tikus Putih (Rattus Norvegicus). Jurnal Ilmiah Ilmu Keolahragaan. 2021;5(1):61-69. DOI: 10.24114/so.v5i1.24311.
Sugga GS, Khan MU, Khanam R. Protective Role of Fibrates in Cardiac Ischemia/Reperfusion. Journal of Advanced Pharmaceutical Technology and Research. 2012;3(3):188–192. DOI: 10.4103/2231-4040.101016.
Downloads
Published
Issue
Section
License
Copyright (c) 2022 Majalah Farmasi dan Farmakologi
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
The copyright to this article is transferred to Universitas Hasanuddin (UNHAS) if and when the article is accepted for publication. The undersigned hereby transfers all rights in and to the paper including without limitation all copyrights to UNHAS. The undersigned hereby represents and warrants that the paper is original and that he/she is the author of the paper, except for material that is clearly identified as to its original source, with permission notices from the copyright owners where required. The undersigned represents that he/she has the power and authority to make and execute this assignment.
We declare that:
- This paper has not been published in the same form elsewhere.
- It will not be submitted anywhere else for publication prior to acceptance/rejection by this Journal.
- A copyright permission is obtained for materials published elsewhere and which require this permission for reproduction.
Furthermore, I/We hereby transfer the unlimited rights of publication of the above-mentioned paper in whole to UNHAS The copyright transfer covers the exclusive right to reproduce and distribute the article, including reprints, translations, photographic reproductions, microform, electronic form (offline, online) or any other reproductions of similar nature.
The corresponding author signs for and accepts responsibility for releasing this material on behalf of any and all co-authors. This agreement is to be signed by at least one of the authors who have obtained the assent of the co-author(s) where applicable. After submission of this agreement signed by the corresponding author, changes of authorship or in the order of the authors listed will not be accepted.
