AKTIVITAS ANTIHIPERURISEMIA EKSTRAK ETANOL 96% RIMPANG TEMU GIRING (Curcuma heyneana Val.) PADA MENCIT JANTAN YANG DIINDUKSI KALIUM OKSONAT DAN JUS HATI AYAM
Keywords:
Temu giring (Curcuma heyneana), zingiberaceae, antihiperurisemia, kalium oksonat, jus hati ayam, Temu giring (Curcuma heyneana), zingiberaceae, antihiperurisemia, kalium oksonat, jus hati ayam.Abstract
Hiperurisemia adalah keadaan dimana kadar asam urat di dalam darah meningkat dan mengalami kejenuhan. Penggunaan tanaman sebagai alternatif pengobatan penyakit hiperurisemia saat ini semakin meningkat. Salah satu tanaman yang diduga memiliki aktivitas antihiperurisemia adalah temu giring (Curcuma heyneana Val.). Penelitian dilakukan untuk mengetahui efek antihiperurisemia ekstrak etanol 96% rimpang temu giring (Curcuma heyneana Val.) pada mencit putih jantan (Mus musculus) yang diinduksi kalium oksonat dan jus hati ayam. Penelitian dilakukan dengan desain eksperimental murni menggunakan 6 kelompok hewan uji, masing-masing kelompok terdiri dari 4 ekor mencit, yaitu kelompok kontrol normal, kontrol negatif, pembanding alopurinol, dan ekstrak etanol 96% temu giring (Curcuma heyneana Val.) dengan dosis masing-masing 50 mg/kgBB, 250 mg/kgBB, dan 500 mg/kgBB. Induksi hiperurisemia dilakukan dengan pemberian jus hati ayam selama 7 hari dan kalium oksonat yang diberikan pada hari ke-8. Pengukuran kadar asam urat dalam serum darah mencit putih jantan dilakukan dengan metode enzimatik menggunakan fotometer klinis dengan panjang gelombang 505 nm. Hasil penelitian menunjukkan ekstrak etanol 96% rimpang temu giring (Curcuma heyneana Val.) dosis 50 mg/KgBB, 250 mg/KgBB, dan 500 mg/KgBB mampu menurunkan kadar asam urat hewan coba secara signifikan (p<0.05). Aktivitas antihiperurisemia ekstrak dosis 50 mg/KgBB, 250 mg/KgBB, dan 500 mg/KgBB masing-masing sebesar 50.71%, 94.29%, dan 136.43%. Aktivitas antihiperurisemia terbaik ditunjukkan oleh ekstrak etanol 96% rimpang temu giring (Curcuma heyneana Val.) dosis 500 mg/KgBB. Penelitian ini mendukung potensi temu giring (Curcuma heyneana Val.) untuk dikembangkan sebagai terapi komplementer hiperurisemia.
References
Benn CL, Dua P, Gurrell R, Loudon P, Pike A, Ian Storer R, et al. Physiology of hyperuricemia and urate-lowering treatments. Front Med. 2018;5(MAY):1–28.
Mattiuzzi C, Lippi G. Recent updates on worldwide gout epidemiology. Clin Rheumatol. 2020;39(4):1061–3.
Salim Z, Munadi E. Info Komoditi Tanaman Obat. Jakarta; 2017.
Jalil M. Temu Giring (Curcuma heyneana Val.): Sebuah Tinjauan Morfologi, Fitokimia, dan Farmakologi. J Biol Educ. 2019;2(2):104.
Putra AMP, Rustifah R, Arsyad M. Uji Daya Hambat Ekstrak Etanol Rimpang Temu Giring (Curcuma Hey Neana Val.) Terhadap Pertumbuhan Escherichia Coli Secara in Vitro. J Ilm Manuntung. 2017;1(1):68.
Marianne M, Patilaya P, Barus BT. Uji Aktivitas Antioksidan Kombinasi Ekstrak Etanol Rimpang Temu Giring (Curcuma heyneana) dan Daun Pugun Tanoh (Curanga Fel-Terrae) Menggunakan Metode Diphenyl Picrylhydrazil(DPPH). Talent Conf Ser Trop Med. 2018;1(2):398–404.
Serrano JL, Figueiredo J, Almeida P, Silvestre S. From Xanthine Oxidase Inhibition to in Vivo Hypouricemic Effect: An Integrated Overview of in Vitro and in Vivo Studies with Focus on Natural Molecules and Analogues. Evidence-based Complement Altern Med. 2020;2020:9–11.
Lin CN, Huang AM, Lin KW, Hour TC, Ko HH, Yang SC, et al. Xanthine oxidase inhibitory terpenoids of Amentotaxus formosana protect cisplatin-induced cell death by reducing reactive oxygen species (ROS) in normal human urothelial and bladder cancer cells. Phytochemistry [Internet]. 2010;71(17–18):2140–6. Available from: http://dx.doi.org/10.1016/j.phytochem.2010.08.012
Zhang C, Wang R, Zhang G, Gong D. Mechanistic insights into the inhibition of quercetin on xanthine oxidase. Int J Biol Macromol [Internet]. 2018;112:405–12. Available from: https://doi.org/10.1016/j.ijbiomac.2018.01.190
Cicero AFG, Fogacci F, Cincione RI, Tocci G, Borghi C. Clinical Effects of Xanthine Oxidase Inhibitors in Hyperuricemic Patients. Med Princ Pract. 2021;30(2):122–30.
Hidayah N, Hasanah F, Gunawan M, Lestari A. Uji Efektifitas Antihiperurisemia Ekstrak Daun Salam (Syzygium polyanthum Wight.) Terhadap Mencit Jantan yang Diinduksi Jus Hati Ayam dan Kalium Oksonat. J Saintika. 2018;18(1):24–31.
Suhendi A, Nurcahyanti, Muhtadi, Sutrisna EM. Aktivitas antihiperurisemia ekstrak air jinten hitam ( Coleus ambonicus Lour ) pada mencit jantan galur balb-c dan standardisasinya. Maj Farm Indones. 2011;22(2):77–84.
Sonia R, Yusnelti Y, Fitrianingsih F. Efektivitas Ekstrak Etanol Daun Durian (Durio zibethinus (Linn.)) sebagai Antihiperurisemia. J Kefarmasian Indones. 2020;10(2):130–9.
Sasongko H, Farida Y, Rohman Efendi N, Pratiwi D, Dwi Setyawan A, Widiyani T. Analgesic Activity of Ethanolic Extracts of Karika Leaves (Carica pubescens) In Vivo Aktivitas Analgesik Ekstrak Etanol Daun Karika (Carica pubescens) Secara In Vivo. J Pharm Sci Clin Res. 2016;01(02):83–9.
Sutyarso, Kanedi M, Rokiban A, Wahyuni A. Anti-hyperuricemic effects of water-soluble fraction of leaf extract of sukun (Artocarpus Altilis) on mice fed purine-rich foods. Biomed Pharmacol J. 2020;13(2):915–9.
Lu J, Dalbeth N, Yin H, Li C, Merriman TR, Wei WH. Mouse models for human hyperuricaemia: a critical review. Nat Rev Rheumatol [Internet]. 2019;15(7):413–26. Available from: http://dx.doi.org/10.1038/s41584-019-0222-x
Feig DI, Mazzali M, Kang DH, Nakagawa T, Price K, Kannelis J, et al. Serum uric acid: A risk factor and a target for treatment? J Am Soc Nephrol. 2006;17(SUPPL. 2):69–73.
Masruroh IN. Uji Aktivitas Antihiperurisemia Ekstrak Metanol Biji Juwet (Syzygium cumini (L.) Skeels) Pada Mencit Jantan Galur Balb-C Hiperurisemia. Universitas Jember; 2016.
Wahyuningsih S, S EY, Karina N. Efek Antihiperurikemia Ekstrak Air Kelopak Bunga Rosela ( Hibiscus sabdariffa L .) pada Tikus Putih Wistar Jantan The Antihyperuricemia Effect of Water Extract of Roselle ( Hibiscus sabdariffa L .) Calyx in Male Wistar Rats. J Farm Sains dan Terap. 2015;2:4–7.
Muhtadi ., Suhendi A, Wahyuningtyas N, Sutrisna E. UJI PRAKLINIK ANTIHIPERURISEMIA SECARA IN VIVO PADA MENCIT PUTIH JANTAN GALUR BALB-C DARI EKSTRAK DAUN SALAM (Syzigium polyanthum Walp) DAN DAUN BELIMBING WULUH (Averrhoa bilimbi L.). Biomedika. 2014;6(1):17–23.
Hilario A, Dente MJ, Fabellon KD, Garcia R, Guiamano F, Herrera A, et al. Ginger Rhizome (Curcuma longa) on Potassium Oxonate-induced Hyperuricemic Mice. Int J Med Heal Sci. 2017;6(2):106–10.
Lee YS, Sung YY, Yuk HJ, Son E, Lee SJ, Kim JS, et al. Anti-hyperuricemic effect of Alpinia oxyphylla seed extract by enhancing uric acid excretion in the kidney. Phytomedicine [Internet]. 2019;62(May):152975. Available from: https://doi.org/10.1016/j.phymed.2019.152975
Abd B, Mohammed A, Askar SJ. ANTIHYPERURICEMIC EFFECT OF ALCOHOLIC GINGER RHIZOME EXTRACT ON POTASSIUM OXONATE , INDUCED HYPERURICEMIC RATS. Plant Arch. 2020;20(2):5459–64.
Kostić DA, Dimitrijević DS, Stojanović GS, Palić IR, Dordević AS, Ickovski JD. Xanthine oxidase: Isolation, assays of activity, and inhibition. J Chem. 2015;1.
Mehta SK, Nayeem N. Natural Xanthine Oxidase Inhibitors for Management of Gout : A Review. Res Rev J Med Heal Sci. 2014;3(Supplement 3):4–13.
Chen Y, Li C, Duan S, Yuan X, Liang J, Hou S. Curcumin attenuates potassium oxonate-induced hyperuricemia and kidney inflammation in mice. Biomed Pharmacother [Internet]. 2019;118(June). Available from: https://doi.org/10.1016/j.biopha.2019.109195
Bupparenoo P, Pakchotanon R, Narongroeknawin P, Asavatanabodee P, Chaiamnuay S. Effect of Curcumin on Serum Urate in Asymptomatic Hyperuricemia: A Randomized Placebo-Controlled Trial. J Diet Suppl [Internet]. 2021;18(3):248–60. Available from: https://doi.org/10.1080/19390211.2020.1757798
Ao GZ, Zhou MZ, Li YY, Li SN, Wang HN, Wan QW, et al. Discovery of novel curcumin derivatives targeting xanthine oxidase and urate transporter 1 as anti-hyperuricemic agents. Bioorganic Med Chem [Internet]. 2017;25(1):166–74. Available from: http://dx.doi.org/10.1016/j.bmc.2016.10.022
Kamsu GT, Pierre SCF, Tagne RS, Kodjio N, Fakam ALN, Gatsing D. Evaluation of the Acute and Sub-chronic Toxicity of the Ethanolic Extract of Curcuma longa (Zingiberaceae) in Wistar Albino Rats Modern Chemistry & Applications. Mod Chem Appl. 2019;7(1):1–9..
Downloads
Published
Issue
Section
License
Copyright (c) 2022 Majalah Farmasi dan Farmakologi
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
The copyright to this article is transferred to Universitas Hasanuddin (UNHAS) if and when the article is accepted for publication. The undersigned hereby transfers all rights in and to the paper including without limitation all copyrights to UNHAS. The undersigned hereby represents and warrants that the paper is original and that he/she is the author of the paper, except for material that is clearly identified as to its original source, with permission notices from the copyright owners where required. The undersigned represents that he/she has the power and authority to make and execute this assignment.
We declare that:
- This paper has not been published in the same form elsewhere.
- It will not be submitted anywhere else for publication prior to acceptance/rejection by this Journal.
- A copyright permission is obtained for materials published elsewhere and which require this permission for reproduction.
Furthermore, I/We hereby transfer the unlimited rights of publication of the above-mentioned paper in whole to UNHAS The copyright transfer covers the exclusive right to reproduce and distribute the article, including reprints, translations, photographic reproductions, microform, electronic form (offline, online) or any other reproductions of similar nature.
The corresponding author signs for and accepts responsibility for releasing this material on behalf of any and all co-authors. This agreement is to be signed by at least one of the authors who have obtained the assent of the co-author(s) where applicable. After submission of this agreement signed by the corresponding author, changes of authorship or in the order of the authors listed will not be accepted.
