Antibacterial Activity of Ethanol Extract of Dragon’s Blood (Daemonorops didymophylla) Against Salmonella typhi and Streptococcus mutants.

Minarni - Minarni Bio
Article History

Submited : June 26, 2020
Published : December 31, 2020

Abstract. Dragon’s blood is essentially a red-colored resin secreted by the fruits of rattan species. Dragon’s blood originated from Indonesia which becomes widespread in the international market is indigenous from Daemonorops sp. Dragon’s blood has been popularly used as traditional medicines. The benefit of dragon’s blood as medicine certainly cannot be separated from the content of secondary metabolites. To confirm such dragon’s blood efficacy, an assessment was already conducted regarding the phytochemical screening and activity dragon’s blood produced by three rattan species, i.e. Daemonorops didymophylla. Ethanol extract of dragon’s blood contains secondary metabolites which are effective as an antibacterial against Salmonella typhi and Streptococcus mutants. This study aims to determine what are the classes of compounds contained in the ethanol extract of dragon’s blood Daemonorops didymophylla which is effective as an antibacterial against the bacteria Salmonella typhi and Streptococcus mutants. Results revealed that the separation of the active compound ethanol extract of dragon’s blood Daemonorops didymophylla, where the separation of compounds through liquid vacuum chromatography has 4 combined fractions. The class of compounds found in the ethanol extract of dragon’s blood Daemonorops didymophylla are flavonoids, triterpenoids and alkaloids. Antibacterial activity of Daemonorops didymophylla dragon’s blood ethanol extract against Salmonella typhi and Streptococcus mutans showed that the higher the concentration the higher the inhibitory power produced, for Salmonella typhi in the 1000 ppm extract obtained an inhibition zone of 13.63 mm with a strong category while Streptococcus mutans in the 1000 ppm extract obtained a inhibition zone of 11.86 mm with a strong category and fraction 1 is the fraction that has the largest inhibition zone than the other three fractions with a large inhibition zone of 14.6 mm in testing Salmonella typhi and 12.1 mm in testing Streptococcus mutants. 

References

  1. REFERENCES
  2. Permenhut 2007 Peraturan Menteri Kehutanan No.: P.35/Menhut-II/2007 tentang Hasil Hutan Bukan Kayu Jakarta.
  3. Toriq U. Senyawa Kimia Penciri Jernang untuk Pembaruan Parameter Standar Nasional Indonesia. Strata 1 (Skripsi); Bogor (ID): Institut Pertanian Bogor 2013.
  4. Soemarna Y. Ekologi dan teknik perkecambahan dan pembibitan rotan jernang pulut (Daemonorops draco (Willd.) Blume). Jurnal Penelitian Hasil Hutan 2009; 6(1): 3-39.
  5. Gupta D, Bleakley B, Gupta RK. Dragon's blood: Botany, chemistry and the rapeuticuses. Journal of Ethnopharmacology 2008; 115(3): 361-380.
  6. Marsh PD. Dental plaque as a biofi lm and a microbial community–implications for health and disease. BMC Oral health 2006; 6(1): p14.
  7. Brooks GF, Butel JS, Morse SA. Mikrobiologi Kedokteran Jawetz, Melnick & Adelberg Ed -23. Jakarta: Penerbit Buku Kedokteran EGC 2007; pp. ISBN-13: 978-0 -07-128735- 7.
  8. Dzubak P, Hajduch M, Vydra D, Hustova A, Kvasnica M, Biedermann D, Markova L, Urban M, Sarek J. Pharmacological activities of natural triterpenoids and their therapeutic implications. Nat.Prod 2006; Rep 23: 394-411
  9. Chung KT, Wong TY, Wei CI, Huang YW, Lin Y. Tannins and human health. Critical Review in Food Science and Nutrition 1998; 38(6): 421-464.
  10. Waluyo PK, Pasaribu G. Aktivitas Antioksidan dan Antikoagulasi Resin Jernang (Antioxidant and Anticoagulation Activities of Dragon’s Blood). Jurnal Penelitian Hasil Hutan 2013; 31(4): 306-315.
  11. Waluyo PK, Pasaribu G. Aktivitas Antijamur Antibakteri dan Penyembuh Luka Ekstrak Resin Jernang. Jurnal Penelitian Hasil Hutan 2015; 33(4): 377-385
  12. Sarman SA. Isolasi Drakorodin Dari Resin Daemonorops draco. Strata 1 (Skripsi): ITB Bandung 2014.
  13. Harborne JB. Metode Fitokimia Penuntun cara modern menganalisis tumbuhan. Penerbit ITB Bandung 1987.
  14. Yanti YN, Mitika S. Uji Efektivitas Antibakteri Ekstrak Etanol Daun Sambiloto (Andrographis paniculata Nees) Terhadap Bakteri Staphylococus aureus. Jurnal Ilmiah Ibnu Sina 2017; 2(1): 158-168.
  15. Tasmin N, Erwin, Kusuma IW. Isolasi identifikasi dan uji toksisitas senyawa flavonoid fraksi kloroform dari daun terap (Artocarpus odoratissimus blanco). Jurnal Kimia FMIPA Unmul 2014; 12(1): 45-52.
  16. Atun S. Metode Isolasi dan Identifikasi Tuktur Senyawa Organik Bahan Alam. Jurnal Konservasi Cagar Budaya Brobudur 2014; 8(2): 53-61
  17. Nugroho BW, Dadang, Prijono D. Pengembangan dan Pemanfaatan Insektisida Alami. Strata 1 (Skripsi). Bogor (ID): Pusat Kajian Pengendalian Hama Terpadu 1999.
  18. Hendayana S. Kimia Pemisahan Metode Kromatografi dan Elektroforesis Modren. PT. Remaja Rosdakarya: Bandung 2006.
  19. Oktaviani EP, Purwijatiningsih E, Pranata FS. Kualitas dan Aktivitas Antioksidan Minuman Probiotik Dengan Variasi Ekstrak Buah Naga Merah (Hyloreceus poluhiruz). Jurnal Teknobiologi 2015; 1(1): 1-15.
  20. Davis WW, Stout TR. Disc plate method of microbiological antibiotic assay. American Society for Microbiology 1971; 22(4): 659-665
  21. Ningsih W. Formualsi dan Uji Aktivitas Antibakteri Edible Film Ekstrak Biji Pinang (Areca catcechu Linn). Jurnal Ilmu Farmasi dan Farmasi Klinik 2018; 15(2): 71-76.
  22. Purwanti S. Aktivitas Antioksidan, Antibakteri dan Antibiofilm Resin Jerang (Daemonorops draco). Strata 1 (Skripsi). Bogor (ID): Institut Pertanian Bogor 2017.
  23. Bobbarala V. Antimicrobial Agents. Intech: Croatia chapter 11 2012; pp 239–254.
  24. Cushnie TPT, Lamb JA. Antimicrobial Activity of Flavonoids. International Journal of Antimicrobial Agents 2005; 26(5): p 343 – 356.
  25. Cowan MM. Plant Products as Antimicrobial Agents. Clinical Microbiology Reviews 1999; 12(4): 564–582.
  26. Leon LD, Lopez MR, Moujir L. Antibacterial Properties of Zeylasterone a Triterpenoid Isolated from Maytenus blepharacles against Staphylococcus aureus. Microbiological Research 2010; 165(8): 617-26.

Downloads

Download data is not yet available.
Fulltext