Test the Activity of Vegetable Oils from Shorea Sumatrana SYM Against Bacteria Salmonella and Bacteria Eschericia Coli

Minarni - Minarni Bio
Article History

Submited : June 26, 2020
Published : December 14, 2020

Abstract. Tengkawang oil obtained from shorea sumatrana Sym fruit contains vegetable oil, extracts of tengkawang oil using n-hexane organic solvents. Tengkawang oil from shorea sumatrana fruit is a genus of dipterocarpacea family shorea which produces high value vegetable oil, is used for food preservatives, canker sores and diarrhea and as cosmetics. This study aims to determine the activity of salmonella bacteria, and escheria coli from tengkawang oil. Tengkawang oil was obtained by shocletation and bacterial testing by diffusion method with various oil concentrations (12.5,25,50 b/v) tengkawang oil mixed with bacterial supposition in NA medium and incubated at 37 0C for 18-34 hours to determine the level minimum inhibition (LMI) of culutation liquid results incubated on SDA (Sabourraud Dextrose Agar) media. The phytochemical screening test was carried out to determine the class of compounds contained in tengkawang oil from shorea Sumatrana. The results of the bacterial activity testing study showed that the LMI of concentrated salmonella bacteria was 12.5% b/v. MIC of 21, concentration of 25% b/v. LMI of 16 and concentration of 50% b/v. The LMI is 15, the test of tengkawang oil activity from shorea sumatrana fruit to Eschericia Coli is at a concentration of 12.5% b/v. LMI of 8, concentration of 25% b/v. LMI of 19 and concentration of 50% b/v. LMI is equal to 17. by using the control as a comparison if compared with the positive control of 10 and control negative equal to 7. The extracts of the pitochemical srening test showed that tengkawang oil from shorea sumatrana fruit contained penicillin, coumarin and flavonoids. The highest activity of tengkawang oil on salmonella bacteria was LMI at 12.5% b/v. The level of LMI was 21 activities using the larger Eschericia Coli LMI bacteria at a concentration of 25% b/v. LMI 19, from the activity test both bacteria were categorized as inhibiting the growth of Salmonella bacteria and Eschericia Coli bacteria.


  2. Kusumaningtyas, Valen Adimurti, Yusnelti. Preserving natural foods from tengkawang seed pulp (shorea sumatrana Sym) and the manufacturing process. IDP000049943. March 02 2018. Patent.
  3. Kusumaningtyas, Sulaeman, Yusnelti. The Fats Potential Of Tengkawang Seeds On Food Mikroba Control In Wet Mixing. Bionatura-Journal of Life and Physical Sciences Vol. 14, No. 2, July 2012: 140- 147 ISSN 1411 - 0903.
  4. Yatnita PC. Salmonella Typhi bacteria and typhoid fever. Journal of Public Health 2011; 6(1): 42-46.
  5. Poeloengan, Andrini M, Susan N, Komala I, Hasnita M. Test of Antibacterial Power of Ethanol Extract of Stem Bungur (Largerstoremia speciosa Press) Against Staphylococcus aureus and Escherichia coli in In Vitro. National Seminar on Animal Husbandry and Veterinary Technology 2007; 8(2) :776–782.
  6. Davis WW, Stout TR. Disc Plate Method of Microbiological Antibiotic. Journal of Microbiology 1971; 22(4): 659-665.
  7. Ningtyas R. Antioxidant test, antibacterial extract of kecombrang leaf (Etlingera elatior (Jack) R. M. Smith) as a natural preservative against Escherichia coli and Staphylococcus aureus. Thesis of the Faculty of Science and Technology of the National Islamic University of Syarif Hidayatullah: Jakarta 2010.
  8. Jenie BSL, Kuswanto. Antimicrobial activity of Angkak pigments produced by Monasnrs purpuracs against several pathogenic microbes and food destroyers. Proceedings of the Permi Annual Scientific Meeting 1994; pp. 53-62.
  9. Ancela RL, Usman P, Evy R. Antibacterial test of Kecombrang (nicolaia speciosa horan) stem extracts against Staphylococcus aureus and Escherichia coli. Department of Agricultural Technology Faculty of Agriculture, University of Riau Postal Code 28293 Ancelal@yahoo.com JOM Faperta 2015; 2(2).
  10. Anggraeni D. Application of kecombrang flower extract (Nicolaia sp. Horan) as a preservative for wet noodles. Thesis, Faculty of Agricultural Technology, Bogor Agricultural University, Bogor 2007.
  11. Adityo R, Kurniawan B, Mustofa S. Test the effect of methanol fraction of kecombrang stem extract (Etlingera elatior) as larvacide on the third instar larvae of Aedes aegypti. MAJORITY (Medical Journal of Lampung University) 2013; 2(5): 2337-3776.
  12. Branen AL, Davidson PM. Antimicrobial in Food. Marcel Dekker Inc. Third Edition. New York 1993; pp 1-706.
  13. Brooks GF, Butel JS, Morse SA. Medical microbiology. Salemba Medika Publisher. Jakarta 2005; pp 273-275.
  14. Harborne, 1987. Phytochemical Method, Guide to Modern Methods of Analyzing Plants. Translator: K. Padmawinata & I. Sudiro. Bandung Institute of Technology 1987; pp 100-103.
  15. Kuete V, Ngameni B; Tangmouo JG, Bolla J. Efflux pumps are evolved in the defense of Gram-negative bacteria Against the natural product of Isobavachalcone and Diospyrone, Antimicrob Agents Chemother 2010; 54(5): 1749-52.
  16. P.M. Davidson, A.L. Branen. Antimicrobia in Food. New York: Marcel Dekker, Inc. 1993
  17. Naufalin R. Study of the antimicrobial properties of kecombrang (Nicolaia speciosa Horan) flowers against various pathogenic microbes and food destroyers. Dissertation of the Postgraduate Program in Bogor Agricultural Institute: Bogor 2005.
  18. Naufalin R, Rukmini HS, Yanto T, Erminawati. Formulation and production of natural preservatives from kecombrang (Nicolaia speciosa Horan). Competency Research Research Report 2009; Directorate General of Higher Education. 15(1): 8–14
  19. Riko A, Lumangkun, Wardenaar E. Benefit Value of Tengkawang (Shorea spp.) For Communities in the District of Embaloh Hilir, Kapuas Hulu Regency, West Kalimantan. Jurnal Hutan Lestari 2013; 1(2): 83-91.
  20. Widodo D, Typhoid F. In: Textbook on Internal Medicine Volume I, Edition VI, Medical Book EGC, Jakarta 2014; pp 1-6.
  21. Waluyo, L. Antimicrobial Test Methanol Fraction and Dietil Ether Leaf Kesum Plant (minus Polygonum). Agripura 2007; pp 31.


Download data is not yet available.