Pengaruh Asam Amino Glisin dan Histidin Terhadap Nilai Koefisien Selektivitas EKT Polipirol-Glutamat Sebagai Sensor Potensiometrik

Authors

  • Abdul Karim Universitas Hasanuddin
  • Damma Salama Universitas Hasanuddin
  • Nurwahyuni Ode Universitas Hasanuddin

DOI:

https://doi.org/10.20956/ica.v2i2.2461

Abstract

A research on the effect of amino acids, glycine and hystidine, on the selectivity of Coated Wire Electrode (EKT)-glutamate has been conducted. Membrane consisted of 0.1 M pyrrole; 0.1 M glutamic acid and 0.1 M HClO4. Analysis of the electrode performance was conducted by looking at the Nernstian factor, detection limit, and solution pH. The effect of amino  acids,  glycine  and hystidine,  on measurement  of glutamic  acid can be  studied  by calculating its selectivity coefficient. Results showed that the electrode gave response of the Nernstian value of 30.24 mV/decade in the concentration range of 10-8  – 10-1  M, detection limit of 2.735 x 10-8  M and pH of 3. Amino acids, glycine and hystidine did not affect the performance of EKT-glutamate with the selectivity coefficient of less than 1.

Keywords: Coated Wire Electrode (EKT)-glutamate, Nernstian, selectivity

References

Colin, C., M. A. Petit, 2002, J. Electrochemical Society, Vol.149, 394-4

Bailey, L. P., 1976, Analysis with Selective Electrodes, Heiden and Son Ltd, New York.

Brett, C. M., dan Brett, M. O., 1993, Electrochemistry Principles Methods and Applications, Oxford University, New York.

Buck, R. P., dan Lindner, E., 2001, Tracing The History of Selective Ion Sensors, Analytical Chemistry, p.88 A97 (http://www.chem.tufts.edu/courses/che m42/History-ISE.pdf 7.06, diakses 24 agustus 2008, 19:06).

Hendayana, S., Kadarohman, A., Sumarna, A. A., dan Supriatna, A., 1994, Kimia Analitik Instrumen Edisi Kesatu, IKIP Semarang Press, Semarang.

Masykur, A., Noviandri, I., dan Buchari, 2002, Usia Pakai dan Pangaruh Oksidator-Raduktor pada Potensial Elektroda Selektif Ion (ESI) Hidrogen dengan Bahan Aktif Antimoni, Alchemy Jurnal Penelitian Kimia, 1(2), 7-19, Universitas Sebelas Maret, Surakarta.

Megawati, 2008, Pembuatan Elektroda Kawat Terlapis Polipirol-Aspartat dan Penggunaannya Sebagai Sensor Aspartat dalam Analisis Jagung, Tesis Tidak Diterbitkan, Program Pascasarjana, Universitas Hasanuddin, Makassar.

Nursyamsiar, 2008, Desain Sensor Kimia (Biosensor) untuk Analisis Asam Glutamat pada Jagung yang dikembangkan di Makassar, Tesis Tidak Diterbitkan, Program Pascasarjana, Universitas Hasanuddin, Makassar.

Wahab, A. W., Buchari, Upe, A., dan Jalaluddin, M. N., 2005, Pengaruh Komposisi Membran Berpendukung PVC terhadap Kinerja Elektroda Selektif Ion (ESI) Hg(II) Menggunakan Ionofor DBDA18C6, Jurnal Penelitian Enjiniring, 11(3), 305-469

Wang, E., Meyerhoff, M. E., dan Yang, V. C., 1995, Optical Detention of Macromolecular Heparin Via Selective Coextaction into Thin Polymeric Films, Anal. Chem, New York.

Widya, E., 2005, Pembuatan Elektroda Tipe Kawat Terlapis Polipirol-Lisin dan Penggunaannya Sebagai Sensor Potensiometrik Lisin, Tesis, Departemen Kimia ITB, Bandung.

Downloads

Published

2009-12-01

Issue

Section

Articles