UTILIZATION OF ETHYL ACETATE FRACTION KETAPANG LEAF (Terminalia catappa) AS BIOREDUCTOR IN SYNTHESIS GOLD NANOPARTICLES AND ANALYSIS ANTIBACTERIAL PROPERTIES
DOI:
https://doi.org/10.20956/ica.v8i2.2470Keywords:
Antibacterial, biosynthesis, characterization of nanoparticles, gold nanoparticles, Terminalia catappa.Abstract
Gold nanoparticles was synthesized by bioreduction method using ethyl acetate fraction of ketapang leaf (Terminalia catappa). Nanoparticles formed were characterized using spectrophotometer UV-Vis, Fourier Transform Infra Red (FTIR) and X-Ray Diffraction (XRD). In the analysis of spectrophotometer UV-Vis gold nanoparticles with a precursor solution concentration of 0.5 mM and 1 mM each has an absorption between 533.5-540 nm and 539-587 nm. Analysis of functional groups of gold nanoparticles was analyzed by Fourier Transform Infra Red (FTIR). Crystal system of gold nanoparticles is cubic with nanoparticles size of 17.13 nm. The evaluation of test antibacterial activity was performed using several bacteria test such as Staphylococcus aureus, Escherichia coli, and Bacillus subtilis. Gold nanoparticles can not inhibit the activity of a test bacterium Staphylococcus aureus, Escherichia coli, and Bacillus subtilis so that differences in the concentration of gold nanoparticles did not affect the inhibition of bacteria.References
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