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Phytochemical analysis, toxicity test by the   brine   shrimp   lethality   test   (BSLT) against Artemia salina L, and antioxidant activity evaluation of the extracts of  Kerehau leaves (Callicarpa longifolia  Lam.)  have  been  carried  out.  The  ethanol  extract  obtained  from  Kerehau  leaves  was concentrated by using a rotary vacuum evaporator. Furthermore, the crude extract was fractionated with n-hexane and ethyl acetate solvent, successively. The phytochemical  analysis of crude extract constitutes alkaloids, phenolics, flavonoids and steroids. n-hexane fraction contains alkaloids, flavonoids and steroids and ethyl acetate fraction contain alkaloids, phenolics and flavonoids. n-Hexane fraction exhibited highest toxic activity with LC50  value 90.05 ppm against Artemia salina L and ethyl acetate fraction has the highest antioxidant activity with IC50 value 38.94 ppm compared with other extracts.


Phytochemical toxicity antioxidant and Artemia salina L

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  1. Harden GJ, editor. 1992. Flora of New South Wales. Vol. 3. Kensington,Australia: New South Wales University Press.
  2. Harley, RM.; Atkins, S.; Budantsev, AL.; Cantino, P.D.; Conn, BJ.; Grayer, R.; Harley, MM.; de Kok, R.; Krestovskaja, T.; Morales, R.; Paton, AJ.; Ryding, O.; Upson, T. Labiatae. In: Kadereit, JW.,editor. 2004, Flowering Plants, Dicotyledons: Lamiales, except Acanthaceae, including Avicenniaceae,The Families and Genera of Vascular Plants. New York: Springer, page 478.
  3. Karamac, M, Bucinski, A., Pegg, R.B., and Amarowicz, R. 2002. Antioxidant and Antiradical Activity Of Ferulate, Czech J. Food Sci. 23, 64-68.
  4. Meyer, B.N., Ferrigny, N.R., Putnam, J.E., Jacobsen, L.B., Nicols, D.E. and McLaughlin, J.L. 1982. Brine Shrimp, A Covenient General Bioassay for Active Plant Contituent. Journal of Medical Plant Research. 45 : 31-34
  5. Novadiana, A., Erwin, dan Pasaribu, S.P, 2013, Uji Toksisitas (Brine Shrimp Lethality Test) Ekstrak dan Isolat Fraksi Kloroform dari daun Karehau (Callicarpa longifolia Lamk.), Prosiding Seminar Nasional Kimia Kalimantan Timur, hal. 134 –
  6. Pasaribu, S.P., Erwin and Istianti, P. 2014, Isolasi dan Identifikasi Senyawa Flavonoid dari Daun Kerehau, Jurnal Kimia Mulawarman, Vol (2), 81-84.
  7. Rasikari, H, 2007, Phytochemistry and arthropod bioactivity of Australian Lamiaceae, Theses, Southern Cross University ePublications@SCU, Australia, page 31.
  8. Setyowati, F. M. 2010. Etnofarmakologi dan Pemakaian Tanaman Obat Suku Dayak Tunjung di Kalimantan Timur.Media Litbang Kesehatan,20, 104-112
  9. Susiati, S., Hoesen, D.S.H, dan Hidayat, A. 2000, Keanekaragaman Tumbuhan yang Berpotensi Sebagai Tanaman Hias di Kutai, Kalimantan Timur, Prosiding Seminar Hari Cinta Puspa dan Satwa Nasional, Kebun Raya Bogor, Bogor, 214-219.
  10. Jones, P.W and Kinhorn, A.D. 2009, Biologically Active Natural Products Of The Genus Callicarpa, Curr Bioact Compd,4(1): 15–32