Optimasi Produksi Enzim Lipase Dari Isolat Aspergillus oryzae

Seniwati Dali (1) , Rauf Patong (2) , Muhammad Noor Djalaluddin (3) , Pirman AP (4)
(1) Departemen of Chemistry, Hasanuddin University, Indonesia,
(2) Departemen of Chemistry, Hasanuddin University, Indonesia,
(3) Departemen of Chemistry, Hasanuddin University, Indonesia,
(4) Department of Chemical Engineering, Ujung Pandang State of Polytechic, Indonesia
https://doi.org/10.70561/ica.v3i1.5969

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Issue
Volume 3 No 1 - June 2010
Submitted
January 25, 2019
Accepted
January 25, 2019
Published
June 1, 2010
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Abstract

Production optimization of lipase enzyme from Aspergillus oryzae isolate on moldy copra has been conducted. Enzyme isolation is carried out after the fungi is activated and cultured on media containing peptone and olive oil at 370C in temperature and 7,0 in pH during 8 days. Production optimization of lipase enzyme is conducted by varying composition of production media, namely: peptone concentration and olive oil and optimal stirring speed. The study results showed that activity of the highest lipase enzyme is acquired on pepton concentration of 1 %, olive oil concentration of 3 % and the stirring speed is 150 rpm. Of concentration optimization of production media and stirring speed is acquired that there is an increasing activity of the lipase enzyme from 13.222 U/mL to 18888U/mL.

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References

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Authors

Seniwati Dali
Departemen of Chemistry, Hasanuddin University
seniwatid@gmail.com (Primary Contact)
Rauf Patong
Departemen of Chemistry, Hasanuddin University
Muhammad Noor Djalaluddin
Departemen of Chemistry, Hasanuddin University
Pirman AP
Department of Chemical Engineering, Ujung Pandang State of Polytechic
Dali, S., Patong, R., Noor Djalaluddin, M., & AP, P. (2010). Optimasi Produksi Enzim Lipase Dari Isolat Aspergillus oryzae. Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta), 3(1), 1–7. https://doi.org/10.70561/ica.v3i1.5969
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