Main Article Content

Abstract

This research aimed to show the percentages of the level of the cholesterol decreased by the convertion product of chitosan enzimatically through in vitro. The research method was started by the demineralization process of the sample in the form of powder of crab shells, and then continued with the de-colorization and de-proteination steps. The next step was de-asetilation enzymatically using de-acetylated chitin which had been isolated from Bacillus licheniformis HSA3-1a bacteria at 50OC for 2 hours until the chitosan was obtained. The research results indicated that the characteristics of the isolated chitosan had water content of 5.45%, ash content 1.53%, N-total of 6.99% and de-acetylation degree of 78.9%, and it showed white yellowish powder form. The results of the test of chitosan as the reducer of cholesterol level by in vitro process showed that 31.18% of the cholesterolwas decreased by 2 mg concentrate of chitosan. Beide, 0.5 mg of simvastatin (as positive control) had decreased by 58.29%. 

Keywords

Rajungan chitosann Bacillus licheniformis HSA3-1a chitin de-acetylase cholesterol

Article Details

How to Cite
Nasir Maidin, A., Natsir, H., & Dali, S. (2019). ENZIMATIC PRODUCTION OF CHITOSAN FROM WASTE OF RAJUNGAN CRAB SHELL AND IT’S APLICATION IN CHOLESTEROL REDUCTION BY IN VITRO TEST. Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta), 10(1), 25-34. https://doi.org/10.20956/ica.v10i1.6404

References

  1. Arif, R.A., Natsir, H., and Dali, S., 2014, Enzymatic Production of Chitosan from the Waste White Shrimp (Penaeus merguiensis) and Antimicrobial Effect for Durability Against Fishballs, Inter. J. Marina Chimica Acta. Vol. 15 (1): 2-7.
  2. Artiningsih, A., Noor, A., and Natsir, H., 2003, Efforts on Chitin Bioconversion of Origin of Crab Crab into Chitosan, J. Marina Chimica Acta, 5 (1): 10-13.
  3. Central Bureau of Statistics, 2017, Crab and Shellfish Exports by Major Destination Countries 2012-2015, (online), www.bps.go.id/linkTabelStatis/view/id/1020, accessed June 20, 2017.
  4. Delaney, J., 1996, Exotic Animal Companion Medicine Handbook for Veterinarians, Zoological Education Network
  5. Dibyantini, R.E., Simorangkir, I., 2016, Effectiveness Test of Rosella Petal Extract (Hibiscus sabdariffa l.) Against Decreasing Cholesterol Levels in Broiler Chicken Blood Serum, Department of Chemistry FMIPA, Medan University, Medan.
  6. Fatmawati, E., 2008, Effect of the Time of Sambiloto Leaf Extract (Andrographis paniculata Ness.) On Cholesterol, HDL Levels and Diabetes Blood Triglycerides (Rattus norvegicus), Faculty of Science and Technology UIN. Malang, 2008
  7. Hargono, et al., 2008, Making Chitosan from Shrimp Shell Waste and Its Application in Reducing Cholesterol in Goat Fat, Reactors, Vol. 12 No. 1, June 2008, p. 53-57,
  8. Hawab, H.M., 2002. Chitosan Can Bind Molecular Cholesterol, Nusa Kimia 2 (1): 25-31.
  9. Liu, J., Zhang, J., Xia, W., 2008, Hypocholesterolaemic effects of different chitosan in vitro and in vivo samples, Science Direct, Food Chemistry vol 107: 419-425.
  10. Natsir, H., Chandra, D., Rukayadi, Y., Suhartono, M.T., Hwang, J.K., Pyun, Y.R., 2002, Biochemical characteristics of chitinase enzyme from Bacillus sp. of Kamojang Crater, Indonesia, J. of Biochem, Mol. Biol and Biophys., 6 (4): 279–282.
  11. Natsir, H., Noor, A., and Asfari, N., 2004, Conversion of Crab Skin Chitin (Scylla serrata) to Chitosan with Chitin Deacetylase Enzyme, Marine Chimica Acta, 6 (1): 6-9.
  12. Natsir, H., Patong, A.R., Suhartono, M.T., and Ahmad A., 2010, Production and Characterization of Chitinase Enzymes From Sulili Hot Spring In South Sulawesi, Bacillus sp. HSA3-1a, Indo J. Chem, 10 (2): 256-260.
  13. Natsir, H., Patong, R., Suhartono, M.T., Ahmad, A., 2013, Isolation and Purification of Thermostable Chitinase Bacillus licheniformis strain HSA3-1a from Sulili Hot Springs in South Sulawesi, Indonesia, Int. J. of Pharm Bio Sci., 4 (3): (B) 1252 - 1259.
  14. Polychronopulus, Evengelos, Panagiotakos, Demosthenes B., and Polystipioti A, 2005, Diet, Lifestyle Factors and Hypercholesterolemia in edible men and women from Cyprus, Journal of Lipids Health Disease, 4 (17): 1-7.
  15. Pratiwi, I. Y., 2011, Effect of Maltodextrin Variation on the Quality of Instant Cinnamon Powder Drinks (Cinnamomum burmanii BI), UAJY, Yogyakarta.
  16. Rismana E., 2001. Chitosan Fiber Binding Fat. http: //www.kompas. com / compass-print / 0301/09 / science and technology / 60155. htm. Retrieved 30 December 2014.
  17. Rudell, LL., Moris, MD., 1973, Determinationof cholesterol using o-phthaladehyde, J Ripid res 14: 364
  18. Rukayadi, Y., 2003, Chitin Deacetylase and its Utilization, Hayati, 9 (4): 130-134.
  19. Sugita, P., et al., 2009, Chitosan Future Material Resources, ITB Press, Bogor.
  20. Sutioso, H., 2012, Utilization of Pectin Isolated from Guava Leaves (Psidium Guajava) in In Vitro and In Vivo Tests Decreased Cholesterol Levels, University of Indonesia, Depok.
  21. Tokuyasu, K, Onishi-Kameyama, M., and Hayashi, K., 1996, Purification and Characterization of Extracellular Chitin Deacetylase from Colletotrichum lindemuthianum, Biosci. Biotech. Biochem. 60 (10): 1598 -1603.