Phytocimia Test And Antibacterial Bioactivity Of Extracts from Stem Bark of Artocarpus integer (Thunb.) Merr

Article History

Submited : August 2, 2019
Published : November 30, 2019

This research aims to identify the compound group and determine the antibacterial activity of n-hexane, chloroform, and methanol fractions of Artocarpus integer (Thunb.) Merr stem bark. All fractions contain groups of terpenoid and steroid compounds. Antibacterial activity was detemined using agar diffusion method with MHA (Muller Hinton Agar) media on Escherichia coli (gram negative) and Staphylococcus aureus (gram positive). Ciprofloxacin was used as a positive control and methanol as a negative control. The three fractions were able to inhibit the growth of Escherichia coli and Staphylococcus aureus. The chloroform fraction showed the highest activity after 48 hours incubation with inhibition zones of 10.8 and 10.4 mm respectively.

References

  1. Boonlaksiri, C., Oonanant, W., Kongsaeree, P., Kittakoop, P., Tanticharoen, M. Thebtaranonth, Y., 2000, An antimalarial stilbene from Artocarpus integer. Pythochemistry, 54: 415-417.
  2. Cordes, J., Wittersheim, M., Harder, J., and Glaser, R., 2014, The Skin's Own Antibiotics. Important Features of Antimicrobial Peptides for Clinical Practice, Hautarzt, 65 (1), 50-55
  3. David and Stout, 1971, Disc Plate Method of Microbiological Antibiotic Essay. Journal of Microbiology, 22(4).
  4. Ganeson, S., Adon, M.N., Jamil, M.M.A., 2017, Artocarpus altilis Compound Based Study for Anti-Cancer Proliferation on the Hela Cell Electroporation Method, Journal of Fundamental and Applied Sciences, 9 (4): 310-318
  5. Hafid, AF, Aoki-Utsubo, C., Permanasari, A.A, Adianti, M., Tumewu, L., Widyawaruyanti, A., Wahyuningsih, SPA, Wahyuni, TS, Lusida, MI, Soetjipto, Hotta, H., 2017 , Antiviral Activity of the Dichloromethane Extracts from Artocarpus heterophyllus Leaves against Hepatitis C Virus, Asian Pacific Journal of Tropical Biomedicine, 7 (7): 633-639.
  6. Hakim, A, 2010. Diversity of Secondary Metabolites from Genus Arto (Moraceae), NUSANTARA BIOSCIENCE, 2 (3): 146- 156.
  7. Hashim, NM, Rahmani, M., Lian, E, GE, Sukari, MA, Yahayu, M., Oktima, W., Ali, AM, Go, R., 2012. Antiproliferative Activity of Xanthones Isolated from Artocarpus obtusu, Page 9.
  8. Hilma, R., Dewi, E.P., Fadhli, H., 2018. Antimicrobial Activity and Antidiabetic Ethanol Extract of Cempedak Fruit Seeds in the Forest (Artocarpus integer (Thunb) Merr). Journal of Photon, 8 (2): 27-36
  9. Lotulung P.D.N ,. Mozef T ,. Risdian C ,. Darmawan A. 2014. In Vitro Antidiabetic Activities Of Extract and Isolated Flavonoid Compounds from Artocarpus altilis (Parkinson) Fosberg. Indo. J. Chem14 (1): 7-11.
  10. Madhavi, Y., Ragahava, R.K.V., Ravi Kiran, C.H., 2013, Studies on Phytochemical Analysis and Antimicrobial Activity of Artocarpus heterophyllus Fruit Latex against Selected Pathogenic Microorganisms, International Journal of Scientific and Engineering Research, 4 (10): 1456-1468.
  11. Nath, P.C., and Boruah, S., 2019, Antimicrobial Activity of Ethanolic and Methanolic Extract of Artocarpus lakoocha Wall. Ex Roxb. (Moraceae) against Five Different Oral Bacterial Strains, International Journal of Current Microbiology and Aplieed Sciences, 8 (3): 1321-1325.
  12. Navarro-Garcia, V.M., Salinas-Sanchez, D.O., Rodriguez-Flores, T.A., Abarca- Vargas, R., Aldana-Llanos, L., 2012, Antimicrobial Activity of Artocarpesin from Artocarpus heterophyllus Lam. against Methiciliin-Resistant Staphylococcus aureus (MRSA), Journal of Medicinal Plants Research, 6 (34): 4879-4882.
  13. Nuntawong, P., Kongkatitham, V., Likhitwitayawuid, K., Mekboonsonglarp, V., Sukrong, S., Tanasupawat, S., Sritularak, B., 2017. New 2-arylbenzofuran from the Bark Root of Artocarpus gomezianus and their α-glucosidase Inhibitory Activity. Natural Product Research. Page1-6.
  14. Pelczar, M.J., and Chan, E.C.S., 1988, Basics of Microbiology, UI Press, Jakarta.
  15. Zakaria,. Soekamto, N.H ,. Shah, Y.M., Firdaus, 2017. Antibacterial activity of the Arthocarpus integer (Thunb) Merr fraction. with the Agar Diffusion Method, E- Journal of Plantation Products Industry (JIHP). 12 (2).

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