Significance of HBsAg Expression in Placental Tissue with Serum Positivity in Hepatitis B Virus-infected Mother
DOI:
https://doi.org/10.20956/nmsj.vi.20242Abstract
Introduction: Hepatitis B virus infection in fetal and newborn may result from the intrauterine vertical transmission. The purpose of our study is to determine the association between HBsAg expression in the placental and HBsAg serum in pregnant women. Methods: Total 67 placental tissues and serum were obtained from HBV-infected mothers. This study was conducted in various hospitals in Makassar, Indonesia from December 2017 until August 2018. Maternal serum and placental tissues were tested by ELISA and Immunohistochemistry staining. Results: 67 samples from placental tissues and serum were categorized into two different levels of serum titer and staining positivity. Twenty nine samples (43.3%) were categorized in low titer of HBsAg positive serum and 38 samples (56.7%) in high titer of HBsAg positive serum. From the total of 67 placental samples, 43 (64.2%) samples had positive expression of HBsAg immunohistochemistry staining and 24 (34.8%) samples had negative expressions. Conclusions: There was a significant correlation between the expressions of HBsAg in the placental tissues and serum titers of HBV-infected mothers that is important in predicting the risk of transplacental transmission. The results of this study can be used as guidelines for the prevention and therapeutic approach of mother-to-child HBV infection.
References
1. Zhang L, Gui X, Wang B, Fan J, Cao Q, Mullane, K, et al. Serological positive markers of hepatitis B virus in femoral venous blood or umbilical cord blood should not be evidence of in-utero infection among neonates. BMC Infect 2016; Dis.16:408. DOI 10.1186/s12879-016-1754-1.
2. Hepatitis: Preventing mother-to-child transmission of the hepatitis B virus. WHO 2022; HTTPS: WWW.WHO.int/about/Policies/privacy
3. Hepatitis B. Department of Communicable Diseases Surveillance and Response. World Health Organization. 2012
4. Devici O, Yula E, Ozer T.T, et al. Investigation of intrauterine transmission of Hepatitis B Virus to children from HBsAg-positive pregnant women. J Med Microbiol and Infection Disease 2011; 1 (1):14-16
5. Vyas AK, Jindal A, Hissar S, Ramakrishna G, Trehanpati N. Immune balance in Hepatitis B Infection: Present and Future Therapies. Scand J Immunol. 2017; 86(1):4-14.
6. Nelson NP, Jamieson DJ, Murphy T V. Prevention of perinatal hepatitis B virus transmission. J Pediatric Infect Dis Soc. 2014; 3(SUPPL1):7–12.
7. Navabakhsh B, Mehrabi N, Estakhri A, et al.. Hepatitis B Virus Infection during Pregnancy: Transmission and Prevention. Middle East J Dig Dis 2011; 3(2): 92–102.
8. Gentile I, Zappulo E, Buonomo A.R., Borgia G. Prevention of mother-to-child transmission of hepatitis B virus and hepatitis C virus. Expert Rev Anti Infect Ther. 2014; 12(7): 775-782. DOI: 10.1586/14787210.2014.920254
9. Mavilia MG, Wu GY. Mechanisms and Prevention of Vertical Transmission in Chronic. J Clin Transl Hepatol 2017; 28:5(2):119-129. doi: 10.14218/JCTH.2016.00067.
10. Vivekanandan P, Samal J, Kandpal M. A simple and rapid method for the quantitation of secreted hepatitis B virions in cell culture models. Indian J Med Microbiol 2015;33(2):290, doi: 10.4103/0255 0857.153568.
11. Kim SW, Roh J, Park CS. Immunohistochemistry for Pathologists: Protocols, Pitfalls, and Tips. J Pathol Transl Med 2016;50(6):411-8, doi: 10.4132/jptm.2016.08.08.
12. Yu M, Jiang Q, Gu X, Ju L, Ji Y, Wu K, et al. Correlation between Vertical Transmission of Hepatitis B Virus and the Expression of HBsAg in Ovarian Folliclesand Placenta. PLos ONE 2013; 8(1): e54246. Doi:10.1371/Journal.pone.0054246.
13. Abdi F, Novin MG, Afrakhteh M, Khorvash F. Hepatitis B and pregnancy: An update review article. World J Obstet Gynecol 2015; 4(1). doi: 10.5317/wjog.v4.i1.1.
14. Bhat P, Anderson DA. Hepatitis B Virus Translocate Across a Trophoblastic Barrier. Journal Of Virology 2007; 7200-7207.
15. Belopolskaya M, Avrutin V, Firsov S, Yakovlev A. HBsAg level and hepatitis B viral load correlation with focus on pregnancy. Annals of Gastroenterology 2015; 28(3): 379-384.
16. Chen Y, Wang LJ. Role of Maternal Viremia and Placental Infection in Hepatitis B Virus Intrauterin Transmision. Microbes Infect 2013; 15(5): 409-415. DOI: 10.1016/j.micinf.2013.02.008.
17. Hou J, Liu Z, Gu F. Epidemiology and Prevention of Hepatitis B Virus Infection. Int J Med Sci 2005; 2(1):50-7.
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Copyright (c) 2023 Jane Magdalena Kalatiku, Berti J Nelwan, Andi Alfian Zainuddin, Cahyono Kaelan, Djumadi Achmad, Rina Masadah
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