THE ASSOCIATION OF POLYMORPHISM PROMOTER VEGF PROMOTER GENE POLYMORPHISM AND VEGF SERUM LEVEL WITH THE TYPE OF AGE-RELATED MACULAR DEGENERATION

Authors

  • Andi M Ichsan Department of Ophthalmology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
  • Habibah S Muhiddin Department of Ophthalmology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
  • Nasrum Massi Department of Clinical Microbiology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
  • Budu Budu Department of Ophthalmology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia

DOI:

https://doi.org/10.20956/nmsj.v1i2.2211

Abstract

ABSTRACT 

Introduction: One of the potential genetic links to Age-related macular degeneration (AMD) is vascular endothelial growth factor (VEGF). The aim of this study was to investigate the association of polymorphism promoter VEGF gene and VEGF serum level with the type of AMD. Methods: Genomic DNA was extracted from peripheral blood of 66 samples, consist of wet and dry AMD, and age-matched controls. DNA was subjected to polymerase chain reaction amplification at the position -460 coupled with the restriction fragment length polymorphism. VEGF serum level was measured by Enzyme-linked Immunosorbent Assay. Results: The polymorphism promoter VEGF gene demonstrated significant association with the type of AMD (p=0.004, OR=3.1 95% CI 1.1-8.6) and the risk for increasing VEGF serum level (p=0.011, OR=3.2 95% CI 1.18.9). Despite there was no significant association between dry and wet AMD with VEGF serum level (p=0.081), but this study revealed significant increased of VEGF serum level in AMD patients, compared to those in control’s serum (p<0.001). Finally, there was coassociation between polymorphism and VEGF level with the type of AMD (p<0.001). Conclusion: This study reveals the significant association between promoter VEGF gene polymorphism and VEGF serum level with the type of AMD of Indonesian patients.  Keywords: AMD, VEGF gene polymorphism; VEGF serum level.

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Published

2017-08-04

How to Cite

Ichsan, A. M., Muhiddin, H. S., Massi, N., & Budu, B. (2017). THE ASSOCIATION OF POLYMORPHISM PROMOTER VEGF PROMOTER GENE POLYMORPHISM AND VEGF SERUM LEVEL WITH THE TYPE OF AGE-RELATED MACULAR DEGENERATION. Nusantara Medical Science Journal, 1(2), 1–9. https://doi.org/10.20956/nmsj.v1i2.2211

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