The Immunoreactivity of recombinant protein MPT63 Mycobacterium tuberculosis as serodiagnostic tuberculosis
DOI:
https://doi.org/10.20956/nmsj.vi.42583Abstract
Introduction: Mycobacterium tuberculosis protein-63 (MPT63) is an extracellular protein encoded by the Rv1926c gene with a size of 16 kDa. MPT63 can specifically induce macrophage activation, stimulate cytokine production (TNF-α and IL-6), and stimulate Th1 cells. The immunogenic properties of MPT63 can be concluded from the presence of a high density of T cell epitopes in the N-terminal immunodominant. This research aims to emphasize and determine the antibody response from the administration of the MPT63 antigen as a candidate in active TB serodiagnostic testing. Methods: This type of research is quantitative and is a diagnostic study. Serum samples were taken from Hasanuddin University Hospital stored biological material with 10 for healthy serum and 36 for sick serum. The determination of optical density values is based on the results of the ELISA reader. The determination of optical density values is based on the results of the ELISA reader. Sensitivity and specificity were tested using the Mc Nemar formula and then they calculated the sensitivity and specificity values manually. Results: Based on the interpretation of the ELISA test from the MPT63 antigen, the antibody reaction response was obtained for a sensitivity of 50% and a specificity of 58.33%. Conclusions: Antibody responses to an antigen that is used indicate that all samples successfully respond well to the serum antigen in the serum of healthy and active tuberculosis but have a sensitivity that is still lacking.
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