EXTENDED SPECTRUM BETA LACTAMASE (ESBL); INDIKATOR RESISTENSI ANTIBIOTIKA GOLONGAN SEFALOSPORIN UNTUK PASIEN TERINFEKSI BAKTERI Pseudomonas aeruginosa di RSUP DR. WAHIDIN SUDIROHUSODO MAKASSAR

Authors

Muhammad Subhan A. Sibadu Jurusan Farmasi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Muhammadiyah, Makassar
M. Natsir Djide Fakultas Farmasi, Universitas Hasanuddin, Makassar
Muh. Nasrum Massi Departemen Mikrobiologi, Fakultas Kedokteran, Universitas Hasanuddin, Makassar
Nurul Muhlisa Mus

Keywords:

Antibiotika Sefalosporin, Pseudomonas aeruginosa, Resistensi, ESBL

Abstract

β-laktamase spektrum luas (ESBL) merupakan suatu kelompok enzim laktamase yang bertanggungjawab terhadap sebagian besar kasus resisten bakteri yang sebagian besar merupakan bakteri gram negatif, terhadap antibiotika β-laktam generasi baru yang kini telah teridentifikasi dalam jumlah besar di seluruh dunia. Penelitian ini bertujuan untuk Memperoleh data prevalensi dari resistensi antibiotika golongan sefalosporin pada bakteri Pseudomonas aeruginosa dan Mengetahui frekuensi kejadian Extended Spectrum Beta Lactamase (ESBL) pada 25 spesimen klinis di RSUP Dr. Wahidin Sudirohusodo Makassar selama periode April – Juli 2017. Pengujian yang dilakukan meliputi uji sensitivitas antimikroba yang dilakukan dengan mengguakan metode difusi agar Kirby-Bauer dan uji produksi ESBL dengan menggunakan metode double disc synergy test (DDST) dan phenotypic confirmatory test. Hasil penelitian menunjukkan bahwa bakteri Pseudomonas aeruginosa berhasil diisolasi dari semua sampel dengan tingkat keakuratan sebesar (90% - 99%). Pada uji sensitivitas antimikroba ditemukan bahwa dari 25 sampel klinik yang diuji terhadap antibiotika sefalosporin, yang telah mengalami resistensi secara berurutan dari yang terbesar adalah cefotaxime 19 sampel (76%), ceftriaxone 16 sampel (64%), dan ceftazidime 7 sampel (28%). Pada uji produksi ESBL ditemukan 21 sampel (84%) positif ESBL pada antibiotika cefotaxime+as. klavulanat, 21 sampel (84%) positif ESBL pada antibiotika ceftriaxone+as.klavunalat, dan 16 sampel (64%) positif ESBL pada antibiotika ceftriaxone.

References

Radji M., 2011, Mikrobiologi Panduan Mahasiswa Farmasi & Kedokteran.Jakarta: Penerbit Buku Kedokteran EGC.

Umadevi S. et al., 2011, Prevalence and antimicrobial susceptibility pattern of ESBL producing Gram Negative. Bacilli. Journal of Clinical and Diagnostic Research, 5 (2): 236.

Alaa H., Al-Charrakh., Salwa J., Al-Awadi, & Ahmed S., 2016, Detection of Metallo-β-Lactamase Producing Pseudomonas aeruginosa Isolated from Public and Private Hospitals in Baghdad. Iraq.Acta Medica Iranica. Volume 54 (2): 107.

Shahanara B. et al., 2013, Detection Of Extended Spectrum β-lactamase In Pseudomonas Spp. Isolated From Two Tertiary Care Hospitals In Bangladesh. BMC Research Notes, 6 (7): 2.

Sivaraman U., Noyal M.J, & Kandha K., 2011, Detection Of Extended Spectrum Beta Lactamases, Ampc Beta Lactamases And Metallobetalactamases In Clinical Isolates Of Ceftazidime Resistant Pseudomonas aeruginosa. Brazilian Journal of Microbiology, (42): 1284 – 1285.

Sennang N., Wildena., & Benny R., 2010, Methicilin Resistent Staphylococcus aureus, Antimicrobial Susceptinility Laboratory Test. Indonesian Journal Of Clinical Pathology And Medical Laboratory, hlm. 5 – 8.

National Committee for Clinical Laboratory Standards., 2000, Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically: approved standard M7-A5 and informational supplement M100-S12. Wayne (PA): National Committee for Clinical Laboratory Standards.

Thomson K.S., 2001, Controversies about extended-spectrum and AmpC beta-lactamases. Emerg Infect Dis Journal, (7): 333 - 6.

Cormican M.G., Marshall S.A., & Jones R.N., 1996, Detection of extendedspectrum β-lactamase (ESBL)-producing strains by the E-test ESBL screen. J Clin Microbiol. 34: 1880 - 4.

Stefaniuk E., Mrowka A., & Hryniewicz W., 2005, Susceptibility Testing and Resistance Phenotypes Detection in Bacterial Pathogens Using The Vitek 2 System. Department Of Epidemiology and Clinical Microbiology, National Institute Of Public Health Chelmska. Polish Journal Of Microbiology, (54): 314 - 316 .

Setiabudy R. Antimikroba. Dalam: Gunawan SG, Setiabudy R, Nafrialdi, Elysabeth, editor., 2012, Farmakologi Dan Terapi. Ed ke-5. Jakarta: Fakultas Kedokteran – UI.

Rupp M.E. & Fey P.D., 2003, Extended Spectrum β-Lactamase (ESBL)-Producing Enterobacteriaceae: Considerations for Diagnosis, Prevention and Drug Treatment. Drugs, 63 (4): 353-365.

Brown, L., et al. 2015. Through The Wall: Extracelluler Vasicies in Gram-Positive Bacteria, Mycobacteria and Fungi. Nature Reviews Microbiolgogy. (13): 623

Shaikh, S., et al. 2015. Antibiotic resistance and extended spectrum beta-lactamases: Types, epidemiology and treatment. Saudi Journal of Biological Science. (22): 93 - 94

Livermore, D.M. 2008. Defining an extended-spectrum b-lactamase (review). Clin Microbiol Infect. (14): 3 – 4

Spanu, T., et al. 2003. Use of the VITEK 2 System for Rapid Identification of Clinical Isolates of Staphylococci from Bloodstream Infections. American Society for Microbiology. (41): 4259.

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Published

2023-05-03

Issue

Vol. 27 No. 1 (2023): MFF

Section

Articles

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