EFEK PEMBERIAN DANGKE TERHADAP KADAR KOLESTEROL DAN TRIGLISERIDA DARAH TIKUS PEMODELAN HIPERKOLESTEROLEMIA DAN HIPERTRIGLISERIDEMIA
Keywords:
dangke, hiperkolesterolemia, hipertrigliseridemia, propiltiourasil, Dangke, propiltiourasil, antihiperkolesterolemia, antihipertrigliseridemiaAbstract
Dangke adalah makanan tradisional Enrekang, Sulawesi Selatan terbuat dari susu dan getah pepaya. Dangke mengandung bakteri asam laktat, yang bermanfaat bagi kesehatan. Penelitian ini bertujuan untuk mngevaluasi pengaruh dangke terhadap hiperkolesterolemia dan hipertrigliseridemia pada hewan model tikus yang diinduksi dengan propiltiourasil (PTU) dan diet tinggi lemak. Dangke dibuat dengan memanaskan susu sapi, ditambahkan getah pepaya hingga menggumpal, dicetak dan difermentasi hingga 3 hari. Induksi hiperkolesterolemia dan hipertrigliseridemia dilakukan pada 30 ekor tikus menggunakan PTU dan kuning telur bebek lalu diberi pakan tinggi lemak selama 28 hari dan dibagi menjadi 5 kelompok yaitu kelompok kontrol tanpa intervensi, kelompok intervensi dangke (0,5, 1 dan 1,5 g per 200 g bobot tikus), serta kontrol intervensi simvastatin (0,2 mg). Pemberian intervensi dangke maupun simvastatin diberikan 14 hari post induksi hiperlipidemia. Nilai kadar kolesterol total dan trigliserida serum setiap kelompok dibandingkan dengan kontrol normal (n=6). Pada kelompok yang diberi terapi dangke menunjukkan penurunan kadar kolesterol total yang signifikan pada dosis 1 g dan 1,5 g, setara dengan pemberian simvastatin. Kadar trigliserida pada kelompok terapi dangke pada dosis 0,5 g dan 1 g menurun signifikan seperti halnya kelompok terapi simvastatin, tetapi tidak pada kelompok dosis 1,5 g. Dapat disimpulkan bahwa terapi dangke dengan dosis 1 gram per 200 gram bobot badan paling efektif menurunkan kadar kolesterol dan trigliserida secara signifikan pada tikus yang diinduksi PTU dan diet tinggi lemak.References
Adawiyah SR, Hafsan H, Nur F, Mustami MK. Resilience of lactic acid bacteria from dangke to bile salts as probiotic candidates. In Proceedings of the National Seminar on Biology. 2015;1(1).
Balitbangkes. Hasil Riset Kesehatan Dasar : Kementerian Kesehatan RI Jakarta. 2018
Bureenok SW, Suksombat, Kawamoto Y. Effects of the fermented juice of epiphytic lactic acid bacteria (FJLB) and molasses on digestibility and rumen fermentation characteristics of ruzigrass (Brachiaria ruziziensis) silages. Livestock Science. 2011;138: 266-271.
Cobos MAAL. deCoss ND, Ramirez SS, Gonzalez, Cerrato RF. Pediococcus acidilactici isolated from the rumen of lambs with rumen acidosis, 16S rRNA identification and sensibility to monensin and lasalocid.. Research in veterinary science. 2011;90: 26–30.
Kumar M, Nagpal R, Kumar R, Hemalatha R, Verma V, Kumar A, Yadav H. Cholesterol-lowering probiotics as potential biotherapeutics for metabolic diseases. Experimental diabetes research, 2012.
Lianto D, Djabir YY, Mustamu BO, Arsyad A, Djabir YY. Vitamin D Was Superior to Omega-3 as Simvastatin Adjuvant in Improving Blood Lipids and Atherogenic Index in Type-I Dyslipidemic Rats. Turkish Journal of Pharmaceutical Science. 2023.0:0 (uncorrected proof)
Nair AB, Jacob S. A simple practice guide for dose conversion between animals and human. Journal of Basic and Clinical pharmacy. 2016;7(2):27.
Nur F, Hafsan H, Paramitasari D. Isolation and Identification of Lactic Acid Bacteria with Probiotic Potential from Dangke Cow's Milk in Enrekang District. Journal of Biotechnology:. 2015;3(1):1-15.
Nur F, Hatta M, Natzir R, Djide MN. (2017). Isolation of lactic acid bacteria as a potential probiotic in dangke, a traditional food from Enrekang, Indonesia. International Journal of Sciences: Basic and Applied Research. 2017;35(1):19-27.
Nuraida L. Winarti S, Hana, Prandimurti E. Evaluasi In Vitro Terhadap Kemampuan Isolat Bakteri Asam Laktat Asal Air Susu Ibu Untuk Mengasimilasi Kolesterol Dan Mendekonjugasi. Jurnal Teknologi Dan Industri Pangan. 2011;22(1):46-52.
Moreno FS, Marcés RM, Vera G, Astier J, Landrier JF, Miguel M. High fat/high glucose diet induces metabolic syndrome in an experimental rat model. Nutrients. 2018;10(10):1502.
Purwanta MLA, Dewi NPAPA, Saraswati MR. Probiotics for type 2 diabetes mellitus: an anti-diabetic intervention to see beyond the gut. Indonesia Journal of Biomedical Science. 2017;11(2):11.
Sasmita S, Djabir YY, Yustisia I, Malaka R, Boy L, Aminuddin A, Kursia S. Potential Use of Fermented Dangke Cheese to Improve Glycemic Control In Rats Fed With A High-Fat Glucose Diet And Propylthiouracil. Azerbaijan Medical Journal. 2023;63(1):7089-7096
Syahputri H, Silalahi, J, Harahap U, Satria D. Antidiabetic Activity of Lactobacillus fermentum Bacteria from Dengke Naniura Goldfish (Cyprinuscarpio) in Nicotinamide-Streptozotosin Induced Rats. Asian Journal of Pharmaceutical Research and Development, 2020;8(4):12-15.
Syah SP, Sumantri C, Arief II, Taufik E. 2017. Isolation and Identification of Indigenous Lactic Acid Bacteria by Sequencing the 16S rRNA from Dangke, A Traditional Cheese from Enrekang, South Sulawesi. Pakistan Journal of Nutrition. 2017;16(5): 384-392.
Salaj R, Štofilová J, Šoltesová A, Hertelyová Z, Hijová E, Bertková I, Bomba A. The effects of two Lactobacillus plantarum strains on rat lipid metabolism receiving a high fat diet. The Scientific World Journal, 2013.
Usman, Hasono A. Bile tolerance, taurocholate deconjugation and binding of cholesterol by Lactobacillus gasseri strain. Journal of Dairy Science. 1999;82:243-248.
Wang G, Huang W, Xia Y, Xiong Z, Ai L. Cholesterol-lowering potentials of Lactobacillus strain overexpression of bile salt hydrolase on high cholesterol diet-induced hypercholesterolemic mice. Food & Function. 2019;10(3):1684-95.
Weijia H, Qiushi Y, Jingyi L, Yi M, Li L, Jie X, Yuhang L, Pingxiang X, Yi C, Ming X, Xiaorong L. Effects of propylthiouracil addition in high fat diet on blood lipid, body weight and body fat of rats. Journal of Capital Medical University. 2018;21;39(3):385.
Wu TY, Wang CH, Tien N, Lin CL, Chu FY, Chang HY, Lim YP. A population-based cohort study on the association of hyperthyroidism with the risk of hyperlipidemia and the effects of anti-thyroid drugs on hepatic gene expression. Frontiers in Medicine.2020;7:228.
Zakariah MA, Malaka R, Laga A, Ako A. Isolation and identification of lactic acid bacteria from dangke a white soft traditional cheese from Enrekang Regency. International Journal of Recent Technology and Engineering. 2019;8(2):4148-51.
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