PENETAPAN KADAR PSEUDOEPHEDRINE HCl, GUAIFENESIN DAN TRIPROLIDINE HCl SECARA SIMULTAN DALAM SEDIAAN SIRUP DENGAN METODE KCKT
Abstract
Dalam rangka mengontrol kualitas dan keamanan obat yang beredar, maka diperlukan metode analisis obat baru. Penelitian ini bertujuan untuk memperoleh metode analisis penetapan kadar campuran triprolidin HCl, pseudoephedrine HCl dan guaifenesin dalam sediaan sirup secara simultan dengan metode KCKT. Kondisi analisis KCKT menggunakan kolom C18. [Waters x bridge (4,6 x 250 mm) 5 μm], pada panjang gelombang 210 nm, kecepatan alir 0,6 ml/menit dengan fase gerak : [methanol : dapar KH2PO4 pH 3,0 (45 : 55)]. Hasil uji presisi (n=10) mempunyai kadar rata-rata dan %RSD untuk pseudoephedrine HCl, guaifenesin dan triprolidine masing masing sebesar 5,86 mg/mL (97,71%) dan 0,44%; 20,19 mg/mL (100,95%) dan 0.37%; 0,26 mg/mL (103,25%) dan 1,02% dari jumlah yang tertera pada etiket. Hasil uji akurasi berdasarkan nilai recovery untuk pseudoephedrine HCl, guaifenesin dan triprolidine mempunyai rentang masing masing 100,74 - 103,67%; 97,03 - 101,33%; 97,03 - 101,73% . Persyaratan recovery yang dapat menjamin hasil uji untuk pseudoephedrine HCl antara 95,0 -105,0% untuk guaifenesine 97,0 – 103,0% dan untuk triprolidin HCl 90,0 - 107,0%. Berdasarkan hasil penelitian ini maka dapat dapat diperoleh metode analisis penetapan kadar pseudoephedrine HCl, guaifenesin dan triprolidine HCl secara simultan dan dapat digunakan sebagai acuan dalam menganalisis sampel.
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