PENGARUH PEMBERIAN EKSTRAK ETANOL DAUN PARANG ROMANG (Boehmeria virgata) SECARA SUBKRONIS ORAL TERHADAP PROFIL HEMATOLOGI TIKUS PUTIH (Rattus Novergicus)
Abstract
Penelitian ini bertujuan untuk mengetahui pengaruh pemberian secara subkronis oral ekstrak etanol daun parang romang selama 28 hari pada profil hematologi tikus putih meliputi kadar eritrosit, leukosit, trombosit dan hemaglobin. Tikus dikelompokkan secara acak menjadi 4 kelompok, masing-masing kelompok yaitu kelompok 1 diberikan aquadest, kelompok 2 ekstrak etanol daun parang romang 250 mg/kgBB, kelompok 3 ekstrak etanol daun parang 500 mg/kg BB dan kelompok 4 ekstrak etanol parang romang 1000 mg/kgBB. Hasil penelitian diperoleh nilai rata-rata tikus jantan dan betina masing-masing yaitu profil kadar eritrosit 8,56 ± 0,33, 8,17 ± 0,29, 7,13 ± 0,15, 6,90 ± 0,99 dan 6,70 ± 0,47, 6,80 ± 0,47, 7,60 ± 0,24, 7,40 ± 0,24. Trombosit 1112,00 ± 53,07, 1099,00 ± 58,62, 1129,60 ± 63,18 dan 1229,00 ± 71,27 dan 805,33 ± 2,16, 873,33 ± 2,81, 1112,30 ± 58,32, 1196,00 ± 1,08, Hemoglobin 15,06 ± 0,57, 14,01 ± 0,28, 12,83 ± 0,14, 12,26 ± 1,81 dan 12,56 ± 1,18, 13,23 ± 0,76, 13,9 ± 0,32, 13,53 ± 0,28. Profil kadar eritrosit, trombosit, hemaglobin pada tikus jantan dan betina masih dalam kisaran normal pada kelompok perlakuan dan kelompok kontrol. Untuk profil kadar leukosit 22,88 ± 3,52, 22,90 ± 1,26, 23,90 ± 0,49, 24,56 ± 4,14 dan 22,76 ± 7,97, 14,46 ± 2,30, 16,79 ± 1,90, 14,58±1,79. Nilai rata-rata leukosit kelompok kontrol dan kelompok perlakuan tikus jantan serta kelompok perlakuan tikus betina tidak berada pada kisaran normal sedangkan untuk kelompok perlakuan tikus betina berada pada kisaran nilai normal. Hasil uji statistik one way anova pemberian ekstrak etanol daun parang romang setelah 28 hari pada profil hematologi tikus jantan dan betina diperoleh Nilai p>0,05 menunjukkan bahwa pemberian ekstrak etanol daun parang romang secara oral selama 28 hari tidak mempengaruhi profil hematologi tikus putih. Dapat disimpulkan bahwa pemberian ekstrak etanol daun parang romang selama 28 hari tidak memiliki efek toksis terhadap profil hematologi tikus putih ( Rattus novergicus).
References
Manggau, M., Hasan, H., Wahyudin, E., Haryono, K., Mufidah. And Lukman. Efek Farmakologi Tanaman Antikanker yang Digunakan oleh Masyarakat Sulawesi Selatan, Balitbangda Sulawesi Selatan. 2012 ; ISBN 978 602 8400 55 8.
Lukman, M., Rusdi, M., Hatta, M., Rahman, L., Subehan. and Manggau, M. Anti proliferation activity of Nanoencapsulated bioadhesive vaginal gel of isolated active Compound (BVI03) from Boehmeria virgata (Forst) guill leaves against human cancer cervix Hela cells. International Journal of Pharma Sciences and Research. 2014; Volume 6. 836-839.
Wardihan, Rusdi, M., Alam, G., Lukman. and Manggau, M. Selective Cytotoxicity Evaluation in Anticancer Drug Screening of Boehmeria virgata (Forst) Guill Leaves to Several Human Cell Lines: HeLa, WiDr, T47D and Vero. Dhaka Univ. J. Pharm. Sci. 2013 ; 12(2): 87-90.
BPOM. Pedoman Uji Toksisitas Nonklinik Secara In Vivo. Badan Pengawas Obat Dan Makanan Republik Indonesia ; 2014.
Ihedioha JI, Ugwuja JI, Noel-Uneke OA, Udeani IJ, Daniel-Igwe G. Reference Values for the Haematology Profile of Conventional Grade Outbred Albino Mice (Mus musculus) in Nsukka, Eastern Nigeria.ARI. 2012 ; vol9(2):1601-1612
Hall Robert L. In: Andress, J.M., (Eds.). Animal Models in Toxicology. New York: Marcell Dekker Inc. page. 791 ;1992.
Harkness, J. E., Turner, P. V. and Vandewoude, S. Harkness and Wagner’s Biology and Medicine of Rabbits and Rodents. 5th ed. Ames : Willey- Blackwell; 2010
Qili, H., Guoming, S,.Keliang, L,. Fangcheng, Z.,Yong, J., Jun, G., Lida, L.,Zhongren, J., Minwu, J.,Huiping, X. Sex-specific reference intervals of hematologic and biochemical analytes in Sprague-Dawley rats using the nonparametric rank percentile method. Journal Plos One. 2017; 1-18.
Douglass, JW.,Wardrop, KJ.Schalm’s Veterinary Hematology. 6th ed. Wiley-Blackwell. p852-887; 2010.
Bo Li B, Jin Y, Xu Y, Wu Y, Xu J, Tu Y. Safety evaluation of tea (Camellia sinensis (L.) O. Kuntze) flower extract: Assessment of mutagenicity, and acute and subchronic toxicity in rats. Journal of Ethnopharmacology. 2011;133:583–90
Smith CA, Andrews CM, Collard JK, Hall DE, Walker AK. Rats and Mouse. In: Color Atlas of Comparative Diagnostic & Experimental Hematology. Barcelona: Wolfe Publishing/Mosby-Year Bok Europe Ltd.pp9-15; 1994
Sireeratawong S, Piyabhan P, Singhalak T, Wongkrajang Y, Temsiririrkkul R, Punsrirat J, et al. Toxicity evaluation of sappan wood extract in rats. J Med Assoc Thail. 2010;93(7):S50-S57
Downloads
Published
Issue
Section
License
The copyright to this article is transferred to Universitas Hasanuddin (UNHAS) if and when the article is accepted for publication. The undersigned hereby transfers all rights in and to the paper including without limitation all copyrights to UNHAS. The undersigned hereby represents and warrants that the paper is original and that he/she is the author of the paper, except for material that is clearly identified as to its original source, with permission notices from the copyright owners where required. The undersigned represents that he/she has the power and authority to make and execute this assignment.
We declare that:
- This paper has not been published in the same form elsewhere.
- It will not be submitted anywhere else for publication prior to acceptance/rejection by this Journal.
- A copyright permission is obtained for materials published elsewhere and which require this permission for reproduction.
Furthermore, I/We hereby transfer the unlimited rights of publication of the above-mentioned paper in whole to UNHAS The copyright transfer covers the exclusive right to reproduce and distribute the article, including reprints, translations, photographic reproductions, microform, electronic form (offline, online) or any other reproductions of similar nature.
The corresponding author signs for and accepts responsibility for releasing this material on behalf of any and all co-authors. This agreement is to be signed by at least one of the authors who have obtained the assent of the co-author(s) where applicable. After submission of this agreement signed by the corresponding author, changes of authorship or in the order of the authors listed will not be accepted.
