FRAKSINASI SENYAWA PENGHAMBAT ENZIM α-GLUKOSIDASE DARI EKSTRAK ETANOL DAUN SAMBUNG NYAWA (Gynura procumbens (Lour) Merr.)
Abstract
Daun sambung nyawa (Gynura procumbens(Lour) Merr) merupakan salah satu tanaman yang memiliki senyawa yang berpotensi sebagai penghambat enzim α-glukosidase atau sebagai obat antidiabetes melitus. Penelitian ini bertujuan untuk memisahkan senyawa yang memiliki aktivitas penghambatan enzim α-glukosidase dan mengetahui jenis kinetika penghambatan berdasarkan grafik Lineweaver-Burk. Pemisahan senyawa berdasarkan tingkat kepolaran pelarut. Uji aktivitas dilakukan pada ekstrak etanol, Fraksi n-heksan fraksi etil asetat dan fraksi air, dan kontrol positif acarbose yang diukur dengan micro plate reader pada panjang gelombang 405 nm. Hasil penelitian menunjukan bahwa nilai IC50 berturut-turut pada ekstrak etanol 6,56 ppm, fraksi n-heksan sebesar 14,97 ppm, fraksi etil asetat 9,33 ppm, fraksi air 13,03 ppm dan acarbose 6,38 ppm serta mekanisme kinetika penghambatan enzim secara uncompetitive.
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