PENETAPAN KURVA STANDAR SENYAWA TETRA HIDROXY ETHYL DISULPHATE (THES) DALAM PLASMA MARMUT (Cavia porcellus) MENGGUNAKAN KCKT
Abstract
Tetra hydroxy ethyl disulphate (THES) merupakan kandidat obat baru sebagai antibiotik yang memiliki mekanisme kerja yaitu ligan sulfat yang ada pada THES akan mengikat peptidoglikan yang ada pada dinding sel bakteri menyebabkan kerusakan pada membran dinding sel bakteri. Senyawa THES ini sangat potensial untuk mengatasi resistensi antibiotik. Penelitian ini bertujuan untuk menentukan kurva regresi linier dalam plasma marmut (Cavia porcellus). Penelitian ini merupakan penelitian eksperimental yang dilakukan pada bulan Mei- September 2018. Penelitian ini menggunakan marmut jantan dengan bobot 400-600 gram. Pengambilan plasma darah marmut melalui vena cava. Pengukuran kadar THES dalam plasma marmut (Cavia porcellus) menggunakan instrumen HPLC pada panjang gelombang 254 nm, fase gerak Asetonitril : Buffer fostat dan fase diam ODS C18 dengan laju alir 1mL/menit. Hasil penelitian menunjukkan seri konsentrasi THES dalam plasma marmut (Cavia porcellus)10 mg/L, 20 mg/L, 30 mg/L, 40, mg/L, dan 50 mg/L berturut- turut diperoleh luas area kromatogram 37878, 27582, 82582, 111490, 157286 sehingga koefisien korelasi THES dalam plasma secara in vitro sebesar r= 0.91 dengan persamaan y = 3227,2x - 13454 dan diperoleh waktu retensi 2,3 menit. Hasil penelitian dapat disimpulkan bahwa konsentrasi kadar THES dalam plasma marmut (Cavia porcellus) memenuhi uji linieritas dalam matriks biologis.References
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