THE IMPACT OF THE FREQUENCY OF 7,12- DIMETHYLBENZ [Á] ANTHRACENE (DMBA) ADMINISTRATION ON THE FORMATION OF DYSPLASIA AND SQUAMOUS CELL CARCINOMA ON THE SKIN OF ALBINO MICE
Diana Muchsin, Khairuddin Djawad, Alwi A Mappiasse, Idham Jaya Ganda, Nasrum Massi, Gemini Alam
ABSTRACT
Introduction: Dosage and frequency of 7,12-dimethylbenz[á] anthracene (DMBA) com- pound exposure suspected to affect the rapid growth of squamous cell carcinoma (SCC) in albino mice. Objective: find out the impact of DMBA exposure to dysplasia and SCC formation on albino mice. Methods: Twenty-five albino mice were divided into five groups, namely: Group 1 is the control (acetone), group 2 is 5 albino mice given four times DMBA
100 µg exposure for eight weeks, group 3 is 5 albino mice were given eight times DMBA
100 µg exposure for eight weeks, group 4 is 5 albino mice were given twelve times DMBA
100 µg exposure for eight weeks, and group 5 is 5 albino mice were given sixteen times DMBA 100 µg exposure for eight weeks. Histopathological examination were conducted to assess histopathological feature including inflammation, dysplasia, and SCC. Results: There is a significant effect of the frequency of DMBA exposure to the clinical figure and against histopathological feature of the skin albino mice.Conclusions: Dosage and fre- quency of DMBA exposure affect the occurence of dysplasia and SCC, which are in- creasingly being DMBA exposure will accelerate the growth of SCC. The frequency of the most widely pose a SCC in DMBA 100 µg sixteen times exposure group, twice a week for eight weeks.
Introduction: Angiogenesis plays an important role in the growth and metastasis of colorectal carcinoma, which is currently one of the targets of cancer therapy. It has been reported that the Endoglin (CD105) involved in angiogenesis and is a powerful marker for angiogenesis in colorectal carcinoma. Level Quantitative angiogenesis in peritumor and an intratumor area is important to know because it is closely related to the micro-environmental factors that influence the occurrence of cancer angiogenesis. The goal of this study to analyze the distribution pattern of angiogenesis in colorectal cancer by comparing the distribution of angiogenesis in peritumor and intratumor areas between well, moderate and poorly differentiated colorectal carcinoma, and between metastasis and non-metastatic colorectal cancer. Methods: This study analyzed fifty samples of resected adenocarcinoma colorectal. Angiogenesis was assessed by the immunohistochemical method using a primary monoclonal antibody Endoglin (CD105). Positive expression of CD105 was assessed through the CD105 protein expression in neovascular endothelial cells, while the distribution pat- tern of angiogenesis assessed by counting the positive expression of CD105 protein in hot spots by using the MVD (microvessel density) in the peritumor and intratumor areas and then performed statistical analysis. Results: There is a significant difference between the quantitative level of an- giogenesis in peritumor and intratumor areas of well (P<0.01), moderate (P<0.01) and poorly (<0.05) differentiated adenocarcinoma. The significant difference between the quantitative levels of angio- genesis in peritumor and intratumor areas of non-metastatic colorectal cancer (P<0.01) and lymph node metastases (<0.05) was found, but not in colorectal cancer with liver metastasis. Conclu- sion: Angiogenesis pattern is more concentrated in peritumor compared to intratumor areas. This showed the role of stromal cells in-angiogenesis. There is significant expression between angio- genesis in peritumor and intratumor areas.
DETECTION OF SHIGELLA SP ON STOOL CHILDREN WITH DIARRHEA USING CULTURE METHODE AND PCR
Rusdianto Rusdianto, Muh Nasrum Massi, Ahyar Ahmad
ABSTRACT
Introduction: Diarrhea is one health problem in the world with mortality and morbidity rates are high, both in developed and developing countries. This research aims to detect Shigella sp in the stool of children with diarrhea using culture method and PCR. Methods: This research is a descriptive with cross-sectional design. A total of 50 samples were obtained from four health centers in Makassar city, i.e Barabaraya, Pampang, Antang Perumnas and Tamangapa from May - July 2016 with rectal swab method. For culture test, the samples were inoculated on medium SSA. While with PCR test, primer icsA (526 bp) was used. Results: The results indicate that, from 50 samples tested by using culture method, there is no sample (0%) posi- tive detected Shigella sp. While the PCR method there 6 samples (24%) positive detected Shigella sp. Conclusion: The sensitivity of PCR in the detection of Shigella sp from the level of dilution 100 - 10-3. Al together, it can be inferred that PCR method is more accurate method detect Shigellasp than other culture methode.
EXPRESSION ANALYSIS OF PHOSPHATASE REGENERATING LIVER-3 (PRL-3) IN OVARIAN CARCINOMA
Rina Masadah, Risma Maharani, Syahrul Rauf
ABSTRACT
Introduction: Ovarian carcinoma is the fifth most caused of gynecological death and its incidence has been shaply increased in the last five years. Phosphatase Regenerating Liver-3 (PRL-3) is a protein that play an important role in malignant cell proliferation, differentiation, and motilily. Methods: This study analized PRL-3 protein expression in paraffin block of ovarian carcinoma tissue sections by immunohistochemical analysis using PRl-3 monoclonal antibody. Results: We found that PRL-3 was expressed in all tissues analized. However, statistical testing did not found any relation between PRL-3 expression and clinical stages, also no relation found between PRL-3 expression and histological differentiation degree of ovarian carcinoma. Conclusion: No relation found between PRL-3 expression in early stage and advance stage of ovarian carcinoma, as well as among various degree of histological differentiation. Study using more variable obviously needed to gain the knowledge about the role of PRL-3 in ovarian carcinoma pathogenesis.
Introduction: Estimated blood loss is an estimation of how much blood is loss during surgery. Surgical procedure requires a preparation of blood stock, but the demand for blood often larger than the actual blood used. This predicament happens because there is no blood requirement protocol being used. This study aims to determine the estimated blood loss during craniotomy procedure and it’s conformity to blood units ordered for craniotomy procedure. Methods: This study is a retrospective study using data from Wahidin Sudirohusodo General Hospitals’ medi- cal records in the period of January 2010-December 2012. We found 89 craniotomy patients that meet the inclusion criteria comprise of 66 men and 23 women. Results: This study showed that the average estimated blood loss in craniotomy was 3.20 units of whole blood (1120 ml) and the average of the demand of blood ordered was 4.0 units of whole blood (1400 ml). There was no mismatch between the amount of blood ordered for surgery and estimation of blood loss (p=0.73). Conclusion: Estimated blood loss in craniotomy procedure in Wahidin Sudirohusodo hospital, Makassar is 3.20 units of whole blood (1120 ml) was in accordance with amount of blood ordered for surgery.
