KADAR ENZIM KOLINESTERASE TIKUS WISTAR (Rattus norvegicus) YANG TERPAPAR SIPERMETRIN

Nursinah Amir, Eddy Suprayitno, Hardoko Hardoko, Happy Nursyam, Fahrul Fahrul

Abstract


Sipermetrin  bersifat  neurotoksin  dan  dapat  mempengaruhi  kadar  enzim kolinesterase. Sipermetrin ditemukan terdapat pada produk Jambal Roti dengan kadar yang melebihi batas maksimum residu yang diperkenankan SNI dan CAC. Penelitian ini bertujuan untuk mengetahui pengaruh sipermetrin terhadap kadar enzim kolinesterase Tikus Wistar (Rattus norvegicus). Tikus diberi perlakuan paparan sipermetrin dengan dosis 0,00 mg/kg (K-), 0,05 mg/kg, 0,60 mg/kg, 1,10 mg/kg, 1,60 mg/kg, 2,15 mg/kg dan daging ikan yangmengandung sipermetrin 1,73 mg/kg (kontrol positif : K+). Kadar enzim kolinesterase darah tikus ditetapkan berdasarkan metode Enzymatic Photometric.  Hasil menunjukkan bahwa sipermetrin berpengaruh terhadap penurunan kadar enzim kolinesterase Tikus Wistar.  Kadar enzim kolinesterase terendah pada perlakuan paparan sipermetrin dosis 2,15 mg/kg yaitu 4059.9 U/L.


References


Ahmad, L., A. Khan, M. Z. Khan and I.

Hussain. 2009. Cypermethrin Induced Anaemia In Male Rabbits. Pakistan Vet. Journal

(4): 191-195

Amir, N., E. Suprayitno, Hardoko and H.

Nursyam. 2014. Cypermethrin Residues on Jambal Roti Product of Giant Catfish (Arius thalassinus Ruppell). International Journal of ChemTech Research 6 (11): 4789

– 4795

Atessahin, A., S. Yilmaz, I. Karahan, I., Pirincci, and B. Tasdemir. 2005. The Effects of Vitamin E and Selenium on Cypermethrin- Induced Oxidative Stress in Rats. Journal Vet Anim Sci 29:

– 391

Baido, D. R. 2010. Nefropati Toksik.

Online (http://dokternetworkangk97.bl ogspot.com/2010/12/nefropati- toksik.html) Diakses tanggal 1 mei 2015

Bhushan, B., P. N. Saxena, and N. Saxena. 2013b. Biochemical and histological changes in cyfluthrin. Arh Hig Rada Toksikol 64: 57–67

Chakravarthi, K., B. R. Naravaneni and G. H. Philip. 2007. Study of Cypermethrin Cytogenesis effects on Human Lymphocytes Using In-Vitro Techniques. J. Appl. Sci. Environ. Manage 11 (2): 77 - 81

Das, R. N. MD. MRCP and S. Parajuli MBBS. 2006. Cypermethrin Poisoning and Anti- cholinergic Medication- A Case Report. Internet Journal of Medical Update 1 (2): 42 – 44

Debbab, M., S. E. Hajjaji, A. H. Aly, A. Dahchour, M. E. Azzouzi and A. Zrineh. 2014. Cypermethrin Residues in Fresh Vegetables: Detection by HPLC and LC- ESIMS and their Effect on Antioxidant Activity. Journal Mater. Environ. Sci. 5: 2257 –

Eraslan, G., M. Kanbur, S. Silici, S. Altinordulu and M. Karabacak. 2008. Effecs of Cypermethrin on Some Biochemical Change in Rats : The Protective Role of Propolis. Exp. Anim. 57 (5): 453 - 460

Jahanbakhshi, A., F. Shaluei and M. Baghfalaki. 2012. Acute Toxicity of Cypermethrin on the Great Sturgeon (Huso huso) Juveniles. World Journal of Fish and Marine Sciences 4 (2): 170-174

Jayakumar, R., A. Nagarjuna, T. Deuraju, and R. Jayantha. 2008. Alteration of haematological Profiles due to cypermethrin Toxicosis in Rana hexadactyla. Journal

Indian Society of Toxicology 4 (2): 18 – 21

Latuszynska, J., S. Luty, G. Raszewski, D. Przebirowska, and M. T. Rodak. 2003. Neurotoxic Effect of Dermally Applied Chlorpyrifos And Cypermethrin. Reversibility of Changes. Ann Agric Environ Med 10: 197-201

Malole, M. B. M. dan C. S. U. Pramono. 1989. Pengantar Hewan Percobaan di Laboratorium. Pusat antar Universitas Bioteknologi IPB. Bogor

Marigoudar, S. R., R. N. Ahmed and M. David. 2009. Cypermethrin induced respiratory and behavioural responses of the freshwater teleost, Labeo rohita (Hamilton). Veterinarski Arhiv 79 (6): 583-590

Masud, S and I. J. Singh. 2013. Effect of Cypermethrin on some hematological parameters and prediction of their recovery in a freshwater Teleost, Cyprinus carpio. African Journal of Environmental Science and Technology 7 (9): 852 – 856

Maulidiniawati, N. dan K. Oginawati. 2015. Pengaruh Paparan Insektisida Organoklorin Terhadap Perubahan Kadar Thyroid Stimulating Hormone (Tsh) Petani Penyemprot Di Kecamatan Kertasari, Kabupaten Bandung. Online (http://www. publikasi.ftsl.itb.ac.id) Diakses tanggal 21 April 2015

Mayasari, S. 2007. Pengaruh Pemberian Asetaminofen Berbagai Dosis Terhadap Kadar Ureum dan Kreatinin Serum Tikus Wistar. UNDIP. Semarang

Mukadam, M. and A. Kulkarni. 2014. Acute Toxicity of Synthetic Pyrethroid Cypermethrin on Protein Content in Estuarine Clam, Marcia Opima (Gmelin, 1791). J Environ Anal Toxicol 4 (2): 1 – 3

Muthuviveganandavel, V. P. Muthuraman, S. Muthu and K. Srikumar. 2011. Individual And Combined Biochemical And Histological Effect Of Cypermethrin And Carbendazim In Male Albino Rats. Journal of Applied Pharmaceutical Science 01 (9): 121 - 129

Ojutiku, R. O., F. P. Asuwaju, I.O. Ayanda, R.A Obande and O.O. Agbelege. Effect Of Acute Toxicity of Cypermethrin on Some biochemical Parameters of Juveniles of Claria Gariepinus (Burchell, 1822). International Journal of Engineering Science Invention 2 (3): 01 - 07

Qodariah, R. N. 2006. Analisis Fungsi Hati dan Ginjal Manusia dengan Photometer 4010. IPB. Bogor

Rahayu, W. P., S. Ma’oen, Suliantari dan S, Fardiaz. 1992. Teknologi Fermentasi Produk Perikanan. IPB. Bogor

Raj, J., Mohineesh, R. Ray, T. D. Dogra, and A. Raina. 2013. Acute Oral Toxicity and Histopathological Study of Combination of Endosulfan and Cypermethrin in Wistar Rats. Toxicol Int. 20 (1) : 61–67

Rochima, E. 2005. Pengaruh Fermentasi Garam Terhadap Karakteristik Jambal Roti. Buletin Teknologi Hasil Perikanan 8 (2): 46 – 55

Rustia, H. N., B. Wispriyono, D. Susanna dan F. N. Luthfiah. 2010. Lama Pajanan Organofosfat Terhadap Penurunan Kadar Enzim Kolinesterase Dalam Darah Petani Sayuran. Makara Kesehatan 14 (2): 95-101

Saber, A. S. and A. Y. Albarakai. 2014.

Effect Of Cinnamon On

Cypermethrin-Induced

Nephrotoxicity In Albino Rats.

International Journal of

Advanced Research 2 (7): 578-

Sari, K.L., Safni dan Zilfa. 2012.

Degradasi Senyawa

Sipermetrin Dalam Insektisida

Ripcord 5 EC Secara Fotolisis

Dengan Penambahan

TIO2/ZEOLIT. Jurnal Kimia

Unand 1 (1): 76-81

Sangha, G.K., K. Kaur, K.S. Khera and B.

Singh, 2011. Toxicological Effects Of Cypermethrin On Female Albino Rats. Toxicol. Int. 18: 5-8

Saxena, P. and A. K. Saxena. 2010.

Cypermethrin Induced

Biochemical Alterations in the

Blood of Albino Rats. Jordan Journal of Biological Sciences 3 (3): 111 - 114

Suharna, C., L. Sya’rani and T. W.

Agustini. 2006. Study of Quality Management System on Jambal Roti Fish Processing In Pangandaran, Ciamis Regency. Jurnal Pasir Laut 2 (1): 13 – 25

Suzan, A. A., M. A. Faten, I. M. Essa and S.K. Majeed. 2012. The Effects of Cypermethrin on Bone and Bone Marrow in Short and Long Treatment in Wild Pigeons (Culumba livia Gaddi). International Journal of Poultry Science 11 (12): 781-786

Velisek, J., T. Wlasow, P. Gomulka, Z.

Svobodova, R. Dobsikova, L. Novotny, and M. Dudzik. 2006. Effects of cypermethrin on rainbow trout (Oncorhynchus mykiss). Veterinarni Medicina

(10): 469–476

Veerappan, M., I. Hwang, and M.

Pandurangan. 2012. Effect Of

Cypermethrin, Carbendazim And Their Combination On Male Albino Rat Serum. International Journal of Experimental Pathology 93 (5):

-369

Wielgomas, B. and J. Krechniak. 2007.

Effect of α-Cypermethrin and Chlorpyrifos in a 28-Day Study on Free Radical Parameters and Cholinesterase Activity in

Wistar Rats. Polish J. of Environ. Stud. 16 (1): 91-95

Wenjun, B. X., Z. Jianmin, C. Xiaoqin, and W. Huoyan. 2007. Effect of Long-Term Fertilization on the Persistence of Cypermethrin in Soil. Better Crops 91 (4): 10 – 11

Wientarsih, I., R. Madyastuti, B. F.

Prasetyo dan D. Firnanda. 2012. Gambaran Serum Ureum dan Kreatinin pada Tikus Putih yang Diberi Fraksi Etil Asetat Daun Alpukat. Jurnal veteriner

(1): 57-62

Winarno, M. W. dan D. Sundari. 2010.

Uji Toksisitas Sub Kronik

Ekstrak Daun Kembang

Sungsang (Gloriosa super L)

terhadap Fungsi Ginjal Tikus

Putih. Buletin Penelitian

Kesehatan 38 (4): 186-191

Yavasoglu, A., F. Sayım, Y. Uyanıkgil, M.

Turgut, and N. U. K. Yavasoglu.

The Pyrethroid Cypermethrin-Induced Biochemical and Histological Alterations in Rat Liver.

Journal of Health Science 52 (6):

– 780


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