PENGHAMBATAN CYTHOPHATIC EFFECT (CPE) PADA SEL BHK-21 YANG TERINFEKSI VIRUS DENGUE SEROTIPE 4 (DENV-4) DENGAN PEMBERIAN EKSTRAK N-HEKSAN STREPTOMYCES SP. GMR22

Diani Mentari; Jaka Widada; Tri Wibawa; Nastiti Wijayanti

doi:10.20956/bioma.v5i2.10292

Issue

Vol. 5 No. 2 (2020)

Issue Published : Jun 30, 2020

Date Log

Submitted

Jun 7, 2020

Published

Jun 30, 2020

PENGHAMBATAN CYTHOPHATIC EFFECT (CPE) PADA SEL BHK-21 YANG TERINFEKSI VIRUS DENGUE SEROTIPE 4 (DENV-4) DENGAN PEMBERIAN EKSTRAK N-HEKSAN STREPTOMYCES SP. GMR22

https://doi.org/10.20956/bioma.v5i2.10292

Diani Mentari

STIKES Guna Bangsa Yogyakarta

Jaka Widada

Fakultas Pertanian, Universitas Gadjah Mada

Tri Wibawa

Fakultas Kedokteran Universitas Gadjah Mada

Nastiti Wijayanti

Fakultas Biologi, Universitas Gadjah Mada

Corresponding Author(s) : Diani Mentari

dianimentari@gmail.com

BIOMA : JURNAL BIOLOGI MAKASSAR, Vol. 5 No. 2 (2020)
Article Published : Jun 30, 2020

Abstract

Negara tropis seperti Indonesia sangat berpotensi sebagai tempat berkembangnya berbagai penyakit menular berbahaya salah satunya Demam Berdarah Dengue (DBD) yang disebabkan oleh infeksi virus dengue. Variasi genetik pada serotipe virus dengue menyebabkan penyakit ini menjadi perhatian dunia medis karena menyebabkan kompleksitas respon imun yang berbeda. Vaksin Dengue (Denvaxia) diperkenalkan oleh WHO pada akhir tahun 2015, namun penggunaannya belum sepenuhnya efektif. Hal ini menyebabkan banyak peneliti berupaya untuk mencari senyawa bioaktif yang memiliki potensi untuk dikembangkan sebagai agen antiviral. Streptomyces sp. GMR22 diketahui menghasilkan senyawa bioaktif dengan spektrum yang luas. Ekstrak n-heksane Streptomyces sp. GMR22 memiliki nilai CC50 yang tidak terlalu tinggi dibandingkan dengan ekstrak Etil Asetat. Hal ini menyebabkan metabolit sekunder yang dihasilkan dapat digunakan untuk analisis lanjut seperti uji antivirus karena aman terhadap sel BHK-21 yang merupakan host virus dengue. Virus DENV-4 merupakan serotipe endemik di Asia Tenggara. Penelitian ini bertujuan untuk mengetahui aktivitas antivirus DENV-4 menggunakan ekstrak n-heksan Streptomyces sp. GMR22. Aktivitas antivirus dilakukan melalui pengamatan sel BHK-21. Hasil pengamatan morfologi sel BHK-21 terinfeksi virus DENV-4 menunjukkan bahwa pemberian ekstrak n-heksan Streptomyces sp. GMR22 mengurangi terbentuknya Cythophatic Effect (CPE). Namun penggunaan konsentrasi 40 µg/mL menyebabkan kematian pada sel BHK-21. Kata kunci: antiviral, DENV-4, metabolit sekunder, Streptomyces sp. GMR22, CPE

References

Agol V.I, 2012. Cytopathic effects : virus-modulated manifestations of innate immunity ?. Trends Microbiol. Trends Microbiol. Dec; 20(12): 570–576.
Ahlquist P, 2002. RNA-dependent RNA polymerases, viruses, and RNA silencing. Science. May 17;296(5571):1270-3.
Alen M.N.F and Schols D, 2012. Dengue Virus Entry as Target for Antiviral Therapy. Journal Of Tropical Medicine. Volume 2012
Alexandra N., Guerrero S., Bello F.J, 2019. Major Article Comparative assessment of the replication efficiency of dengue, yellow fever , and chikungunya arboviruses in some insect and mammalian cell lines. Rev Soc Bras Med Trop. Apr 25(52).
Dewi B.E., Naiggolan L., Putri D.H., Rachmayanti N., Albar S., Indriastuti N.T., Sjamsuridzal W., Sudiro T.M. 2014. Characterization of dengue virus serotype 4 infection in Jakarta, Indonesia. Southeast Asian J Trop Med Public Health. Jan: 45(1):53-61
Escobar-Herrera J., JoséMedina-Ramírez F., LorenaGutiérrez-Escolano A, 2007. Short communication A carboxymethyl-cellulose plaque assay for feline calicivirus. 146:393–6. Journal of Virological Methods. 146: 393-396.
Harris E, Roberts T.G., Smith L., Selle J., Kramer L.D., Valle S., Sandoval E., Balmaseda A, 1998. Typing of Dengue Viruses in Clinical Specimens and Mosquitoes by Single-Tube Multiplex Reverse Transcriptase PCR. J Clin Microbiol. Sep;36(9):2634-9.
Li Y, Tao P., Liu Y., Jiang J, 2012. Geldanamycin , a ligand of heat shock protein 90 , inhibits herpes simplex virus type 2 replication both in vitro and in vivo. The Journal of Antibiotics. 65 (10): 509–512.
Liu C., and Wu S, 2012. Mosquito and Mammalian Cells Grown on Microcarriers for Four-Serotype Dengue Virus Production : Variations in Virus Titer, Plaque Morphology, and Replication Rate Biotechnol Bioeng. Mar 5;85(5):482-8.
McCormick K.D., Liu S., Jacobs J.L., Marques E.T.A., Sluis-Cremer, Jr.N, Wang T, 2012. Development of a Robust Cytopathic Effect-Based High-Throughput Screening Assay To Identify Novel Inhibitors of Dengue Virus. Antimicrob Agents Chemother. 56(6):3399–401.
Mentari D, 2016. Antivirus Activity Of N-Hexane Extract From Streptomyces Sp. GMR22 Against Dengue Virus Serotype 1. Tesis. Program Pascasarjana Fakultas Biologi UGM
Mentari D, Naima M., Wulansari R., Widada J., Nuringtyas T.R., Wibawa T., Wijayanti N, 2019. Pengaruh Perbedaan Metode Ekstraksi Metabolit Sekunder Streptomyces sp . GMR22 terhadap Toksisitas pada Sel BHK-21. Pharmacon: Jurnal Farmasi Indonesia. 2019;16(1):1–10.
Morens D.M, Halstead S.B., Repik, P.M, Putvatana R., Raybourne N, 1985. Simplified Plaque Reduction Neutralization Assay for Dengue Viruses by Semimicro Methods in BHK-21 Cells : Comparison of the BHK Suspension Test with Standard Plaque Reduction Neutralization. J Clin Microbiol. 1985 Aug;22(2):250-4.
Paisal, Herman H., Arifin A.Y., Ardiansyah A, Hanum S, Khairiah, Zuardi M., Yasir, 2015. Serotipe virus Dengue di Provinsi Aceh Dengue virus serotype in Aceh Province. Aspirator. 7(1): 7-12
Panec M and Katz D.S, 2006. Plaque Assay Protocols [Internet]. 2006. Available from: https://www.asmscience.org/content/education/protocol/protocol.3073
Praditya D.F, 2014. Potensi Aktivitas Antiviral Ekstrak Etil Asetat Dan Ekstrak Air Metabolit Sekunder Aktinomisetes Terhadap Virus Dengue Serotipe-2 (Denv-2). Tesis. Program Pascasarjana Bioteknologi UGM
Supardan D., Widada J., Wibawa T., Wijayanti N., 2016. Uji Viabilitas Virus Dengue Serotipe 3 Pada Beberapa Galur Sel (Cell-Line). Biota. 9(1)
Taslim N, Arsunan A.A., Ishak H., Nasir S, Usman A.N, 2018. Diversity of Dengue Virus Serotype in Endemic Region of South Sulawesi Province. Journal of Tropical Medicine. (1):1-4
Wang C., Liu, Luo J, Hua, Gao Y., Sun Y., Luo F, Liu X, He H, 2017. Geldanamycin Reduces Acute Respiratory Distress Syndrome and Promotes the Survival of Mice Infected with the Highly Virulent H5N1 Influenza Virus. Front Cell Infect Microbiol. Jun 15(7): 267.
WHO, 2017. Dengue vaccine: WHO position paper, July 2016 – recommendations. Vaccine. World Health Organization.Nur Insana Imaniar, 2013. Aktivitas Antivirus Metabolit Sekunder Aktinomisetes Laut dan Tanah Terhadap Virus Dengue Serotipe-1. Thesis. Program Pascasarjana Bioteknologi UGM
Zamree I., Drakes N., Rohani A., Lee H.L, 2005. Sensitivity of Aedes albopictus C6 / 36 cells line for the detection and infectivity titration of dengue virus. Trop Biomed. 22(2):217-9.

References

Agol V.I, 2012. Cytopathic effects : virus-modulated manifestations of innate immunity ?. Trends Microbiol. Trends Microbiol. Dec; 20(12): 570–576.

Ahlquist P, 2002. RNA-dependent RNA polymerases, viruses, and RNA silencing. Science. May 17;296(5571):1270-3.

Alen M.N.F and Schols D, 2012. Dengue Virus Entry as Target for Antiviral Therapy. Journal Of Tropical Medicine. Volume 2012

Alexandra N., Guerrero S., Bello F.J, 2019. Major Article Comparative assessment of the replication efficiency of dengue, yellow fever , and chikungunya arboviruses in some insect and mammalian cell lines. Rev Soc Bras Med Trop. Apr 25(52).

Dewi B.E., Naiggolan L., Putri D.H., Rachmayanti N., Albar S., Indriastuti N.T., Sjamsuridzal W., Sudiro T.M. 2014. Characterization of dengue virus serotype 4 infection in Jakarta, Indonesia. Southeast Asian J Trop Med Public Health. Jan: 45(1):53-61

Escobar-Herrera J., JoséMedina-Ramírez F., LorenaGutiérrez-Escolano A, 2007. Short communication A carboxymethyl-cellulose plaque assay for feline calicivirus. 146:393–6. Journal of Virological Methods. 146: 393-396.

Harris E, Roberts T.G., Smith L., Selle J., Kramer L.D., Valle S., Sandoval E., Balmaseda A, 1998. Typing of Dengue Viruses in Clinical Specimens and Mosquitoes by Single-Tube Multiplex Reverse Transcriptase PCR. J Clin Microbiol. Sep;36(9):2634-9.

Li Y, Tao P., Liu Y., Jiang J, 2012. Geldanamycin , a ligand of heat shock protein 90 , inhibits herpes simplex virus type 2 replication both in vitro and in vivo. The Journal of Antibiotics. 65 (10): 509–512.

Liu C., and Wu S, 2012. Mosquito and Mammalian Cells Grown on Microcarriers for Four-Serotype Dengue Virus Production : Variations in Virus Titer, Plaque Morphology, and Replication Rate Biotechnol Bioeng. Mar 5;85(5):482-8.

McCormick K.D., Liu S., Jacobs J.L., Marques E.T.A., Sluis-Cremer, Jr.N, Wang T, 2012. Development of a Robust Cytopathic Effect-Based High-Throughput Screening Assay To Identify Novel Inhibitors of Dengue Virus. Antimicrob Agents Chemother. 56(6):3399–401.

Mentari D, 2016. Antivirus Activity Of N-Hexane Extract From Streptomyces Sp. GMR22 Against Dengue Virus Serotype 1. Tesis. Program Pascasarjana Fakultas Biologi UGM

Mentari D, Naima M., Wulansari R., Widada J., Nuringtyas T.R., Wibawa T., Wijayanti N, 2019. Pengaruh Perbedaan Metode Ekstraksi Metabolit Sekunder Streptomyces sp . GMR22 terhadap Toksisitas pada Sel BHK-21. Pharmacon: Jurnal Farmasi Indonesia. 2019;16(1):1–10.

Morens D.M, Halstead S.B., Repik, P.M, Putvatana R., Raybourne N, 1985. Simplified Plaque Reduction Neutralization Assay for Dengue Viruses by Semimicro Methods in BHK-21 Cells : Comparison of the BHK Suspension Test with Standard Plaque Reduction Neutralization. J Clin Microbiol. 1985 Aug;22(2):250-4.

Paisal, Herman H., Arifin A.Y., Ardiansyah A, Hanum S, Khairiah, Zuardi M., Yasir, 2015. Serotipe virus Dengue di Provinsi Aceh Dengue virus serotype in Aceh Province. Aspirator. 7(1): 7-12

Panec M and Katz D.S, 2006. Plaque Assay Protocols [Internet]. 2006. Available from: https://www.asmscience.org/content/education/protocol/protocol.3073

Praditya D.F, 2014. Potensi Aktivitas Antiviral Ekstrak Etil Asetat Dan Ekstrak Air Metabolit Sekunder Aktinomisetes Terhadap Virus Dengue Serotipe-2 (Denv-2). Tesis. Program Pascasarjana Bioteknologi UGM

Supardan D., Widada J., Wibawa T., Wijayanti N., 2016. Uji Viabilitas Virus Dengue Serotipe 3 Pada Beberapa Galur Sel (Cell-Line). Biota. 9(1)

Taslim N, Arsunan A.A., Ishak H., Nasir S, Usman A.N, 2018. Diversity of Dengue Virus Serotype in Endemic Region of South Sulawesi Province. Journal of Tropical Medicine. (1):1-4

Wang C., Liu, Luo J, Hua, Gao Y., Sun Y., Luo F, Liu X, He H, 2017. Geldanamycin Reduces Acute Respiratory Distress Syndrome and Promotes the Survival of Mice Infected with the Highly Virulent H5N1 Influenza Virus. Front Cell Infect Microbiol. Jun 15(7): 267.

WHO, 2017. Dengue vaccine: WHO position paper, July 2016 – recommendations. Vaccine. World Health Organization.Nur Insana Imaniar, 2013. Aktivitas Antivirus Metabolit Sekunder Aktinomisetes Laut dan Tanah Terhadap Virus Dengue Serotipe-1. Thesis. Program Pascasarjana Bioteknologi UGM

Zamree I., Drakes N., Rohani A., Lee H.L, 2005. Sensitivity of Aedes albopictus C6 / 36 cells line for the detection and infectivity titration of dengue virus. Trop Biomed. 22(2):217-9.

Issue

Vol. 5 No. 2 (2020)

Date Log

PENGHAMBATAN CYTHOPHATIC EFFECT (CPE) PADA SEL BHK-21 YANG TERINFEKSI VIRUS DENGUE SEROTIPE 4 (DENV-4) DENGAN PEMBERIAN EKSTRAK N-HEKSAN STREPTOMYCES SP. GMR22

Corresponding Author(s) : Diani Mentari

Abstract

References

Downloads