MODEL TIKUS DIABETES YANG DIINDUKSI STREPTOZOTOCIN-SUKROSA UNTUK PENDEKATAN PENELITIAN DIABETES MELITUS GESTASIONAL
DOI:
https://doi.org/10.30597/mkmi.v12i1.550Keywords:
Streptozotocin, sukrosa, Gestasional Diabetes Melitus (GDM)Abstract
Diabetes melitus gestasional (DMG) merupakan intoleransi glukosa pada berbagai tingkatan yang terjadi selama kehamilan. DMG pada wanita meningkatkan risiko kematian sebelum kelahiran pada ibu dan bayinya, tingkat kesakitan pada ibu dan meningkatkan risiko berkembangnya DM tipe-2 setelah melahirkan. Penggunaan hewan coba alternatif dengan metode yang tepat dalam penelitian DMG dengan pendekatan DM tipe-2 telah banyak dilakukan dan cukup mendesak. Tujuan penelitian ini untuk mengetahui apakah induksi Streptozotocin-sukrosa dapat menghasilkan DM tipe-2 pada tikus yang dapat digunakan sebagai pendekatan dalam penelitian DMG. Peneltian ini bersifat eksperimental, tikus Sprague Dawley jantan berjumlah 45 ekor berusia 6 minggu dialokasikan pada 2 kelompok: kontrol (n=5) dan diabetes (n=40). Kelompok kontrol diinjeksi secara intraperitoneal dengan phosphat buffer saline (PBS) tanpa sukrosa dan kelompok diabetes diinjeksi secara intraperitonela dengan streptozotocin (STZ) dosis 40 mg/kg berat badan dan diberikan 30% sukrosa secaraad libitum, pengukuran glukosa darah puasa dan berat badan dilakukan tiap 3 hari. Setelah minggu ke-4 perlakuan, terdapat 24 tikus pada kelompok diabetes dengan glukosa darah puasa diatas 126 mg/dL dan menunjukkan perbedaan signifikan glukosa darah (p<0.05)dan peningkatan berat badan (p<0.05) dibandingkan dengan kelompok kontrol. Induksi STZ-sukrosa dapat menghasilkan DM tipe-2 pada tikusSprague dawley Jantan dan dapat digunakan sebagai pendekatan peneltian DMG.References
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